ID RBBP_ARATH Reviewed; 547 AA.
AC Q5E915; B9DHL4; Q84W60; Q9LJC6;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 24-JAN-2024, entry version 128.
DE RecName: Full=Protein RBL {ECO:0000303|PubMed:21423667};
DE AltName: Full=COMPASS-like H3K4 histone methylation complex component RBL {ECO:0000303|PubMed:21423667};
DE AltName: Full=RBBP5-like protein {ECO:0000303|PubMed:21423667};
DE Short=AtRbBp5 {ECO:0000303|PubMed:23284292};
DE AltName: Full=Retinoblastoma-binding protein-like {ECO:0000303|PubMed:21423667};
GN Name=RBL {ECO:0000303|PubMed:21423667};
GN OrderedLocusNames=At3g21060 {ECO:0000312|Araport:AT3G21060};
GN ORFNames=MSA6.10 {ECO:0000312|EMBL:BAB01449.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAX12875.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 266-547.
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH TRO AND WDR5A, AND
RP SUBCELLULAR LOCATION.
RX PubMed=21423667; DOI=10.1371/journal.pgen.1001330;
RA Jiang D., Kong N.C., Gu X., Li Z., He Y.;
RT "Arabidopsis COMPASS-like complexes mediate histone H3 lysine-4
RT trimethylation to control floral transition and plant development.";
RL PLoS Genet. 7:E1001330-E1001330(2011).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Wassilewskija;
RX PubMed=23284292; DOI=10.1371/journal.pgen.1003111;
RA Ding Y., Ndamukong I., Xu Z., Lapko H., Fromm M., Avramova Z.;
RT "ATX1-generated H3K4me3 is required for efficient elongation of
RT transcription, not initiation, at ATX1-regulated genes.";
RL PLoS Genet. 8:E1003111-E1003111(2012).
CC -!- FUNCTION: Promotes the expression of FLC and FLC homologs to repress
CC the floral transition (PubMed:21423667). Promotes WRKY70 and LTP7 genes
CC epigenetic methylation (e.g. H3K4me3) and subsequent expression
CC (PubMed:23284292). {ECO:0000269|PubMed:21423667,
CC ECO:0000269|PubMed:23284292}.
CC -!- SUBUNIT: Part of a complex composed of TRO, RBL and WDR5A. Interacts
CC with TRO and WDR5A, but not with WDR5B. This complex is formed during
CC both vegetative and reproductive development.
CC {ECO:0000269|PubMed:21423667}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21423667}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in root tips, shoot apices,
CC vascular tissues, developing embryos and endosperms.
CC {ECO:0000269|PubMed:21423667}.
CC -!- DISRUPTION PHENOTYPE: Eearly flowering (PubMed:23284292). Significantly
CC reduced trimethylated 'Lys-4' of histone H3 (H3K4me3) levels at the 5'-
CC ends of WRKY70 and LTP7 genes leading to reduced transcript
CC accumulation (PubMed:23284292). {ECO:0000269|PubMed:23284292}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01449.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP000604; BAB01449.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76455.1; -; Genomic_DNA.
DR EMBL; BT004200; AAO42218.1; -; mRNA.
DR EMBL; BT021105; AAX12875.1; -; mRNA.
DR EMBL; AK317567; BAH20231.1; -; mRNA.
DR RefSeq; NP_188743.3; NM_113000.6.
DR AlphaFoldDB; Q5E915; -.
DR SMR; Q5E915; -.
DR STRING; 3702.Q5E915; -.
DR iPTMnet; Q5E915; -.
DR PaxDb; 3702-AT3G21060-1; -.
DR ProteomicsDB; 236506; -.
DR EnsemblPlants; AT3G21060.1; AT3G21060.1; AT3G21060.
DR GeneID; 821658; -.
DR Gramene; AT3G21060.1; AT3G21060.1; AT3G21060.
DR KEGG; ath:AT3G21060; -.
DR Araport; AT3G21060; -.
DR TAIR; AT3G21060; RBL.
DR eggNOG; KOG1273; Eukaryota.
DR HOGENOM; CLU_032142_2_2_1; -.
DR InParanoid; Q5E915; -.
DR OMA; DYEDDIM; -.
DR OrthoDB; 3686044at2759; -.
DR PhylomeDB; Q5E915; -.
DR PRO; PR:Q5E915; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q5E915; baseline and differential.
DR Genevisible; Q5E915; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0048188; C:Set1C/COMPASS complex; IEA:InterPro.
DR GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IMP:TAIR.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 2.
DR InterPro; IPR037850; RBBP5/Swd1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR44040; RETINOBLASTOMA-BINDING PROTEIN 5; 1.
DR PANTHER; PTHR44040:SF1; RETINOBLASTOMA-BINDING PROTEIN 5; 1.
DR Pfam; PF00400; WD40; 4.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..547
FT /note="Protein RBL"
FT /id="PRO_0000431781"
FT REPEAT 21..60
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 65..104
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 214..253
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 285..332
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 334..373
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REGION 466..547
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 467..481
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 487..508
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 220
FT /note="K -> E (in Ref. 3; AAO42218)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 547 AA; 60142 MW; 7D2B3C5ACBF23375 CRC64;
MNAPIIDPLQ GDFPEVIEEY LEHGVIKCVA FNHRGSLLAA GCADGGCVIW DFETRGIAKE
IRDNDCSAAI TSVSWSKYGH RLLVSAADKS LTLWDVSTGE KIARTILQQT PLQARLNPGL
SSPSLCLACP LSSAPMIVDF DIDCTTLLPV SVPEMPDVLA PPQRSKCPES NPPFSPAAAC
FNKCGDLVYI GNSKGEILIV DYKSVRVLAL VSASGAAPVK NIVFSRNGQY LLTNSHDRTI
RIYENLLPAK NVLKSLEDLG KNIDGLDGIE KMKTVGSKCL TLFREFQDSV TKMHWKAPCF
SGDGEWVVGG SACKGEHKIY IWDRAGHLVK ILEGPKEALI DLAWHPVHPI IVSVSLAGLV
YIWAKDYTEN WSAFAPDFKE LEENEEYVER EDEFDLIPET EKVKVLDVNE DEEVDIDTVE
KDAFSDSDMS VEELRYLPAE PIPDTNDQQD NLVESIKLIE GQISASPASE EAGQNGHHAS
SPQAEEMGET RGKRKRKPSE KAMELQAEKA KPLKGSGKTV RAKNRAAFDQ ETDDSINGGD
DDDDAYY
//
