ID Q5GNZ0_PHOAN Unreviewed; 308 AA.
AC Q5GNZ0;
DT 01-MAR-2005, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2005, sequence version 1.
DT 13-SEP-2023, entry version 75.
DE RecName: Full=DNA-directed RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00015972};
DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418};
DE AltName: Full=RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00033070};
DE AltName: Full=Transcriptase subunit alpha {ECO:0000256|ARBA:ARBA00032524};
DE Flags: Fragment;
GN Name=rpoA {ECO:0000313|EMBL:CAH57267.1};
OS Photobacterium angustum.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Photobacterium.
OX NCBI_TaxID=661 {ECO:0000313|EMBL:CAH57267.1};
RN [1] {ECO:0000313|EMBL:CAH57267.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Type strain: LMG 8455 {ECO:0000313|EMBL:CAH57267.1};
RX PubMed=16151093; DOI=10.1128/AEM.71.9.5107-5115.2005;
RA Thompson F.L., Gevers D., Thompson C.C., Dawyndt P., Naser S., Hoste B.,
RA Munn C.B., Swings J.;
RT "Phylogeny and molecular identification of vibrios on the basis of
RT multilocus sequence analysis.";
RL Appl. Environ. Microbiol. 71:5107-5115(2005).
RN [2] {ECO:0000313|EMBL:ABR12691.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 25915 {ECO:0000313|EMBL:ABR12691.1}, and ATCC 33975
RC {ECO:0000313|EMBL:ABR12692.1};
RA Ast J.C., Cleenwerck I., Engelbeen K., Urbanczyk H., Thompson F.L.,
RA De Vos P., Dunlap P.V.;
RT "Photobacterium kishitanii sp. nov., a luminous marine bacterium symbiotic
RT with deep-sea fishes.";
RL Int. J. Syst. Evol. Microbiol. 57:2073-2078(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550};
CC -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC {ECO:0000256|ARBA:ARBA00007123}.
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DR EMBL; EF415579; ABR12691.1; -; Genomic_DNA.
DR EMBL; EF415580; ABR12692.1; -; Genomic_DNA.
DR EMBL; AJ842538; CAH57267.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5GNZ0; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd06928; RNAP_alpha_NTD; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR HAMAP; MF_00059; RNApol_bact_RpoA; 1.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR011773; DNA-dir_RpoA.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR011260; RNAP_asu_C.
DR InterPro; IPR036643; RNApol_insert_sf.
DR NCBIfam; TIGR02027; rpoA; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF03118; RNA_pol_A_CTD; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF47789; C-terminal domain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000313|EMBL:ABR12691.1};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 16..227
FT /note="DNA-directed RNA polymerase RpoA/D/Rpb3-type"
FT /evidence="ECO:0000259|SMART:SM00662"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:CAH57267.1"
FT NON_TER 308
FT /evidence="ECO:0000313|EMBL:CAH57267.1"
SQ SEQUENCE 308 AA; 33983 MW; B5325D9CE367F42F CRC64;
FLKPRLVDIE QVSTTHAKVT LEPLERGFGH TLGNALRRIL LSSMPGCAVT EVEIDGVLHE
YSTKEGVQED ILEILLNLKG LAVKVEGKDE VILTLNKSGA GPVVAGDITH DGDVEIANPE
HVICHITDDN AELSMRIKVE RGRGYVPASA RIHTEEDERP IGRLLVDATY SPVDRIAYAV
EAARVEQRTD LDKLVIDMET NGTLDPEEAI RRAATILAEQ LDAFVDLRDV RVPEEKEEKP
EFDPILLRPV DDLELTVRSA NCLKAEAIHY IGDLVQRTEV ELLKTPNLGK KSLTEIKDVL
ASRGLSLG
//
