ID Q5I0S9_XENTR Unreviewed; 463 AA.
AC Q5I0S9; A0A6I8SE20; A0A6I8SR75; F6XS86;
DT 15-FEB-2005, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2005, sequence version 1.
DT 27-MAR-2024, entry version 137.
DE RecName: Full=Elongation factor 1-alpha {ECO:0000256|RuleBase:RU000325};
GN Name=eef1a2 {ECO:0000313|EMBL:AAH88010.1,
GN ECO:0000313|Ensembl:ENSXETP00000042318,
GN ECO:0000313|RefSeq:NP_001011418.1, ECO:0000313|RefSeq:XP_031749927.1,
GN ECO:0000313|Xenbase:XB-GENE-975439};
GN Synonyms=eef1al {ECO:0000313|RefSeq:NP_001011418.1,
GN ECO:0000313|RefSeq:XP_031749927.1}, ef1a
GN {ECO:0000313|RefSeq:NP_001011418.1,
GN ECO:0000313|RefSeq:XP_031749927.1}, stnl
GN {ECO:0000313|RefSeq:NP_001011418.1,
GN ECO:0000313|RefSeq:XP_031749927.1};
GN ORFNames=XENTR_v90030110mg {ECO:0000313|EMBL:OCA15304.1};
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364 {ECO:0000313|EMBL:AAH88010.1};
RN [1] {ECO:0000313|RefSeq:NP_001011418.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=12454917; DOI=10.1002/dvdy.10174;
RA Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W.,
RA Richardson P.;
RT "Genetic and genomic tools for Xenopus research: The NIH Xenopus
RT initiative.";
RL Dev. Dyn. 225:384-391(2002).
RN [2] {ECO:0000313|EMBL:AAH88010.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Whole body {ECO:0000313|EMBL:AAH88010.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:OCA15304.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Nigerian {ECO:0000313|EMBL:OCA15304.1};
RG US DOE Joint Genome Institute (JGI-PGF);
RA Ottilar R., Schmutz J., Salamov A., Cheng J.F., Lucas S., Pitluck S.,
RA Gundlach H., Guo Y., Haberer G., Nasrallah J., Mayer K.F.X.,
RA van de Peer Y., Weigel D., Grigoriev I.V.;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:OCA15304.1, ECO:0000313|Ensembl:ENSXETP00000042318}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nigerian {ECO:0000313|EMBL:OCA15304.1,
RC ECO:0000313|Ensembl:ENSXETP00000042318};
RX PubMed=20431018; DOI=10.1126/science.1183670;
RA Hellsten U., Harland R.M., Gilchrist M.J., Hendrix D., Jurka J.,
RA Kapitonov V., Ovcharenko I., Putnam N.H., Shu S., Taher L., Blitz I.L.,
RA Blumberg B., Dichmann D.S., Dubchak I., Amaya E., Detter J.C., Fletcher R.,
RA Gerhard D.S., Goodstein D., Graves T., Grigoriev I.V., Grimwood J.,
RA Kawashima T., Lindquist E., Lucas S.M., Mead P.E., Mitros T., Ogino H.,
RA Ohta Y., Poliakov A.V., Pollet N., Robert J., Salamov A., Sater A.K.,
RA Schmutz J., Terry A., Vize P.D., Warren W.C., Wells D., Wills A.,
RA Wilson R.K., Zimmerman L.B., Zorn A.M., Grainger R., Grammer T.,
RA Khokha M.K., Richardson P.M., Rokhsar D.S.;
RT "The genome of the Western clawed frog Xenopus tropicalis.";
RL Science 328:633-636(2010).
RN [5] {ECO:0000313|EMBL:OCA15304.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Nigerian {ECO:0000313|EMBL:OCA15304.1};
RA Sessions A., Jenkins J., Mitros T., Lyons J.T., Dichmann D.S., Robert J.,
RA Harland R.M., Rokhsar D.S.;
RT "WGS assembly of Xenopus tropicalis.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|Ensembl:ENSXETP00000094469}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (MAY-2020) to UniProtKB.
RN [7] {ECO:0000313|RefSeq:NP_001011418.1, ECO:0000313|RefSeq:XP_031749927.1}
RP IDENTIFICATION.
RC STRAIN=Nigerian {ECO:0000313|RefSeq:XP_031749927.1};
RC TISSUE=Liver and blood {ECO:0000313|RefSeq:XP_031749927.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: This protein promotes the GTP-dependent binding of aminoacyl-
CC tRNA to the A-site of ribosomes during protein biosynthesis.
CC {ECO:0000256|ARBA:ARBA00003982, ECO:0000256|RuleBase:RU000325}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC subfamily. {ECO:0000256|ARBA:ARBA00007249,
CC ECO:0000256|RuleBase:RU000325}.
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DR EMBL; BC088010; AAH88010.1; -; mRNA.
DR EMBL; KV460890; OCA15304.1; -; Genomic_DNA.
DR RefSeq; NP_001011418.1; NM_001011418.1.
DR RefSeq; XP_031749927.1; XM_031894067.1.
DR STRING; 8364.ENSXETP00000018161; -.
DR PaxDb; 8364-ENSXETP00000042318; -.
DR DNASU; 496898; -.
DR Ensembl; ENSXETT00000042318; ENSXETP00000042318; ENSXETG00000019567.
DR Ensembl; ENSXETT00000074698; ENSXETP00000095283; ENSXETG00000019567.
DR Ensembl; ENSXETT00000083465; ENSXETP00000094469; ENSXETG00000019567.
DR GeneID; 496898; -.
DR KEGG; xtr:496898; -.
DR AGR; Xenbase:XB-GENE-975439; -.
DR CTD; 1917; -.
DR Xenbase; XB-GENE-975439; eef1a2.
DR eggNOG; KOG0052; Eukaryota.
DR HOGENOM; CLU_007265_3_5_1; -.
DR OMA; RDMGQTV; -.
DR OrthoDB; 5477300at2759; -.
DR TreeFam; TF300304; -.
DR Proteomes; UP000008143; Chromosome 10.
DR Bgee; ENSXETG00000019567; Expressed in skeletal muscle tissue and 12 other cell types or tissues.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd01883; EF1_alpha; 1.
DR CDD; cd03693; EF1_alpha_II; 1.
DR CDD; cd03705; EF1_alpha_III; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 2.
DR HAMAP; MF_00118_A; EF_Tu_A; 1.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004539; Transl_elong_EF1A_euk/arc.
DR InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR NCBIfam; TIGR00483; EF-1_alpha; 1.
DR PANTHER; PTHR23115:SF228; ELONGATION FACTOR 1-ALPHA 2; 1.
DR PANTHER; PTHR23115; TRANSLATION FACTOR; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF03143; GTP_EFTU_D3; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 2: Evidence at transcript level;
KW Elongation factor {ECO:0000256|RuleBase:RU000325,
KW ECO:0000313|EMBL:AAH88010.1};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134, ECO:0000256|RuleBase:RU000325};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU000325};
KW Protein biosynthesis {ECO:0000256|RuleBase:RU000325,
KW ECO:0000313|EMBL:AAH88010.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000008143}.
FT DOMAIN 5..242
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
FT REGION 444..463
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 463 AA; 50465 MW; A4974EF41DC01034 CRC64;
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL
DKLKAERERG ITIDISLWKF ETNKYYITII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV
GEFEAGISKN GQTREHALLA YTLGVKQLIV GINKMDSTEP PYSEKRYDEI VKEVSAYIKK
IGYNPATVPF VPISGWHGDN MLEPSPNMPW FKGWKVERKE GNANGVSLLE ALDTILPPTR
PTDKPLRLPL QDVYKIGGIG TVPVGRVETG ILKPGMVVTF APVNITTEVK SVEMHHEALS
EALPGDNVGF NVKNVSVKDI RRGNVCGDSK SDPPQEAAGF TSQVIILNHP GQISAGYSPV
IDCHTAHIAC KFAELKEKID RRSGKKLEDN PKSLKSGDAA IVEMIPGKPM CVESFSQYPP
LGRFAVRDMR QTVAVGVIKN VEKKSGGAGK VTKSAQKAQK AGK
//
