ID Q5XJ23_DANRE Unreviewed; 187 AA.
AC Q5XJ23;
DT 23-NOV-2004, integrated into UniProtKB/TrEMBL.
DT 23-NOV-2004, sequence version 1.
DT 27-MAR-2024, entry version 119.
DE RecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase {ECO:0000256|RuleBase:RU367024};
DE Short=dUTPase {ECO:0000256|RuleBase:RU367024};
DE EC=3.6.1.23 {ECO:0000256|RuleBase:RU367024};
DE AltName: Full=dUTP pyrophosphatase {ECO:0000256|RuleBase:RU367024};
GN Name=dut {ECO:0000313|EMBL:AAH83489.1,
GN ECO:0000313|RefSeq:NP_001006005.1,
GN ECO:0000313|ZFIN:ZDB-GENE-041010-93};
GN Synonyms=zgc:103772 {ECO:0000313|RefSeq:NP_001006005.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AAH83489.1};
RN [1] {ECO:0000313|EMBL:AAH83489.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain {ECO:0000313|EMBL:AAH83489.1}, and PCR rescue
RC {ECO:0000313|EMBL:AAI64977.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|RefSeq:NP_001006005.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=16109975;
RA Woods I.G., Wilson C., Friedlander B., Chang P., Reyes D.K., Nix R.,
RA Kelly P.D., Chu F., Postlethwait J.H., Talbot W.S.;
RT "The zebrafish gene map defines ancestral vertebrate chromosomes.";
RL Genome Res. 15:1307-1314(2005).
RN [3] {ECO:0000313|RefSeq:NP_001006005.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=16373575; DOI=10.1126/science.1117637;
RA Whitehead G.G., Makino S., Lien C.L., Keating M.T.;
RT "fgf20 is essential for initiating zebrafish fin regeneration.";
RL Science 310:1957-1960(2005).
RN [4] {ECO:0000313|RefSeq:NP_001006005.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=18335061;
RA Sreenivasan R., Cai M., Bartfai R., Wang X., Christoffels A., Orban L.;
RT "Transcriptomic analyses reveal novel genes with sexually dimorphic
RT expression in the zebrafish gonad and brain.";
RL PLoS ONE 3:e1791-e1791(2008).
RN [5] {ECO:0000313|RefSeq:NP_001006005.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=23306105;
RA Liu C., Wang Q., Liang K., Liu J., Zhou B., Zhang X., Liu H., Giesy J.P.,
RA Yu H.;
RT "Effects of tris(1,3-dichloro-2-propyl) phosphate and triphenyl phosphate
RT on receptor-associated mRNA expression in zebrafish embryos/larvae.";
RL Aquat. Toxicol. 128-129:147-157(2013).
RN [6] {ECO:0000313|RefSeq:NP_001006005.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=24198278;
RA Recher G., Jouralet J., Brombin A., Heuze A., Mugniery E., Hermel J.M.,
RA Desnoulez S., Savy T., Herbomel P., Bourrat F., Peyrieras N., Jamen F.,
RA Joly J.S.;
RT "Zebrafish midbrain slow-amplifying progenitors exhibit high levels of
RT transcripts for nucleotide and ribosome biogenesis.";
RL Development 140:4860-4869(2013).
RN [7] {ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [8] {ECO:0000313|RefSeq:NP_001006005.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26562049;
RA Ma Z., Yu Y., Tang S., Liu H., Su G., Xie Y., Giesy J.P., Hecker M., Yu H.;
RT "Differential modulation of expression of nuclear receptor mediated genes
RT by tris(2-butoxyethyl) phosphate (TBOEP) on early life stages of zebrafish
RT (Danio rerio).";
RL Aquat. Toxicol. 169:196-203(2015).
RN [9] {ECO:0000313|RefSeq:NP_001006005.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=25565004;
RA Liu H., Tang S., Zheng X., Zhu Y., Ma Z., Liu C., Hecker M., Saunders D.M.,
RA Giesy J.P., Zhang X., Yu H.;
RT "Bioaccumulation, biotransformation, and toxicity of BDE-47, 6-OH-BDE-47,
RT and 6-MeO-BDE-47 in early life-stages of zebrafish (Danio rerio).";
RL Environ. Sci. Technol. 49:1823-1833(2015).
RN [10] {ECO:0000313|RefSeq:NP_001006005.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27189481;
RA Pasquier J., Cabau C., Nguyen T., Jouanno E., Severac D., Braasch I.,
RA Journot L., Pontarotti P., Klopp C., Postlethwait J.H., Guiguen Y.,
RA Bobe J.;
RT "Gene evolution and gene expression after whole genome duplication in fish:
RT the PhyloFish database.";
RL BMC Genomics 17:368-368(2016).
RN [11] {ECO:0000313|RefSeq:NP_001006005.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27776230;
RA Wu Y., Su G., Tang S., Liu W., Ma Z., Zheng X., Liu H., Yu H.;
RT "The combination of in silico and in vivo approaches for the investigation
RT of disrupting effects of tris (2-chloroethyl) phosphate (TCEP) toward core
RT receptors of zebrafish.";
RL Chemosphere 168:122-130(2017).
RN [12] {ECO:0000313|RefSeq:NP_001006005.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=29145436;
RA Yilmaz O., Patinote A., Nguyen T.V., Com E., Lavigne R., Pineau C.,
RA Sullivan C.V., Bobe J.;
RT "Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in
RT zebrafish (Danio rerio).";
RL PLoS ONE 12:e0188084-e0188084(2017).
RN [13] {ECO:0000313|RefSeq:NP_001006005.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Involved in nucleotide metabolism via production of dUMP, the
CC immediate precursor of thymidine nucleotides, and decreases the
CC intracellular concentration of dUTP so that uracil cannot be
CC incorporated into DNA. {ECO:0000256|RuleBase:RU367024}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dUTP + H2O = diphosphate + dUMP + H(+); Xref=Rhea:RHEA:10248,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:61555, ChEBI:CHEBI:246422; EC=3.6.1.23;
CC Evidence={ECO:0000256|RuleBase:RU367024};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU367024};
CC -!- PATHWAY: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP
CC route): step 2/2. {ECO:0000256|ARBA:ARBA00005142,
CC ECO:0000256|RuleBase:RU367024}.
CC -!- SIMILARITY: Belongs to the dUTPase family.
CC {ECO:0000256|ARBA:ARBA00006581, ECO:0000256|RuleBase:RU367024}.
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DR EMBL; BC083489; AAH83489.1; -; mRNA.
DR EMBL; BC164977; AAI64977.1; -; mRNA.
DR RefSeq; NP_001006005.1; NM_001006005.1.
DR GeneID; 449835; -.
DR KEGG; dre:449835; -.
DR AGR; ZFIN:ZDB-GENE-041010-93; -.
DR CTD; 1854; -.
DR ZFIN; ZDB-GENE-041010-93; dut.
DR OrthoDB; 1343066at2759; -.
DR UniPathway; UPA00610; UER00666.
DR Proteomes; UP000000437; Chromosome 18.
DR GO; GO:0004170; F:dUTP diphosphatase activity; IBA:GO_Central.
DR GO; GO:0000287; F:magnesium ion binding; IBA:GO_Central.
DR GO; GO:0006226; P:dUMP biosynthetic process; IBA:GO_Central.
DR GO; GO:0046081; P:dUTP catabolic process; IBA:GO_Central.
DR CDD; cd07557; trimeric_dUTPase; 1.
DR Gene3D; 2.70.40.10; -; 1.
DR InterPro; IPR008181; dUTPase.
DR InterPro; IPR029054; dUTPase-like.
DR InterPro; IPR036157; dUTPase-like_sf.
DR InterPro; IPR033704; dUTPase_trimeric.
DR NCBIfam; TIGR00576; dut; 1.
DR PANTHER; PTHR11241; DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE; 1.
DR PANTHER; PTHR11241:SF0; DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE; 1.
DR Pfam; PF00692; dUTPase; 1.
DR SUPFAM; SSF51283; dUTPase-like; 1.
PE 2: Evidence at transcript level;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU367024};
KW Magnesium {ECO:0000256|RuleBase:RU367024};
KW Metal-binding {ECO:0000256|RuleBase:RU367024};
KW Nucleotide metabolism {ECO:0000256|RuleBase:RU367024};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437}.
FT DOMAIN 57..185
FT /note="dUTPase-like"
FT /evidence="ECO:0000259|Pfam:PF00692"
SQ SEQUENCE 187 AA; 20107 MW; 1A3BDFBC3081BC68 CRC64;
MSVRRVSAAV LGRLNGCLLG RGAEVTEAVS PHKRAKSDAV NGAEERAVLK FAKLTEHATT
PSRGSNRAAG YDLYSAYDYS IGPMDKTLVK TGIQIAVPHG YYGRVAPRSG LAVKHFVDVG
AGVVDEDYRG NLGVVIFNFN KEPFEVKKGD RIAQLICEKI CYPDLQELQT LDETERGAGG
FGSTGTN
//
