ID Q5YVF8_NOCFA Unreviewed; 303 AA.
AC Q5YVF8;
DT 23-NOV-2004, integrated into UniProtKB/TrEMBL.
DT 23-NOV-2004, sequence version 1.
DT 27-MAR-2024, entry version 102.
DE SubName: Full=Putative enoyl-CoA hydratase/isomerase family protein {ECO:0000313|EMBL:BAD57833.1};
GN Name=echA14 {ECO:0000313|EMBL:BAD57833.1};
GN OrderedLocusNames=NFA_29860 {ECO:0000313|EMBL:BAD57833.1};
OS Nocardia farcinica (strain IFM 10152).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Nocardia.
OX NCBI_TaxID=247156 {ECO:0000313|EMBL:BAD57833.1, ECO:0000313|Proteomes:UP000006820};
RN [1] {ECO:0000313|EMBL:BAD57833.1, ECO:0000313|Proteomes:UP000006820}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IFM 10152 {ECO:0000313|EMBL:BAD57833.1,
RC ECO:0000313|Proteomes:UP000006820};
RX PubMed=15466710; DOI=10.1073/pnas.0406410101;
RA Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K.,
RA Shiba T., Hattori M.;
RT "The complete genomic sequence of Nocardia farcinica IFM 10152.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004).
CC -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC {ECO:0000256|ARBA:ARBA00002994}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:58856; EC=4.2.1.17;
CC Evidence={ECO:0000256|ARBA:ARBA00023709};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC ChEBI:CHEBI:137480; EC=4.2.1.17;
CC Evidence={ECO:0000256|ARBA:ARBA00023717};
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000256|ARBA:ARBA00005254, ECO:0000256|RuleBase:RU003707}.
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DR EMBL; AP006618; BAD57833.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5YVF8; -.
DR STRING; 247156.NFA_29860; -.
DR KEGG; nfa:NFA_29860; -.
DR eggNOG; COG1024; Bacteria.
DR HOGENOM; CLU_009834_7_2_11; -.
DR Proteomes; UP000006820; Chromosome.
DR GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.12.10; Lyase 2-enoyl-coa Hydratase, Chain A, domain 2; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR PANTHER; PTHR43459:SF1; EG:BACN32G11.4 PROTEIN; 1.
DR PANTHER; PTHR43459; ENOYL-COA HYDRATASE; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000313|EMBL:BAD57833.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000006820}.
SQ SEQUENCE 303 AA; 32007 MW; 88F191396F644402 CRC64;
MGTRWSPDYV GWGRGRVILA RLSDGVVPVH RIECGGPMSE PVLVERHGAT VVWTLNSPRA
RNPISEPETI AALESAVAAA EADPDVRVAI LTGAGAAFSS GGNIKHMRDK AGMFAGGPAD
LRPGYRHGIQ RIPKALYDCE IPTIAAVNGP AIGAGCDLAL MCDMRIAAHS AVFAESFVKV
GLIPGDGGAW LLPRAVGMAR ASEMAFTGDA IDAATALEWG LVSRVVPDAE LLPAAHALAD
RVAANPPHVL RMTKRLIREG QHQRLESLLE LSAAMQAIAH TTGDHHEAVA AMLDKRPPRF
TGR
//
