ID Q66HW2_DANRE Unreviewed; 532 AA.
AC Q66HW2; A0A8M1N1X1;
DT 11-OCT-2004, integrated into UniProtKB/TrEMBL.
DT 11-OCT-2004, sequence version 1.
DT 27-MAR-2024, entry version 151.
DE SubName: Full=EH domain-containing protein 1 {ECO:0000313|RefSeq:NP_001004578.1};
DE SubName: Full=EH-domain containing 1 {ECO:0000313|EMBL:AAH81648.1};
DE SubName: Full=EH-domain-containing 1b {ECO:0000313|Ensembl:ENSDARP00000020181};
GN Name=ehd1b {ECO:0000313|Ensembl:ENSDARP00000020181,
GN ECO:0000313|RefSeq:NP_001004578.1,
GN ECO:0000313|ZFIN:ZDB-GENE-030131-2069};
GN Synonyms=cb862 {ECO:0000313|RefSeq:NP_001004578.1}, ehd1
GN {ECO:0000313|RefSeq:NP_001004578.1}, ehd3
GN {ECO:0000313|RefSeq:NP_001004578.1}, ehd3l
GN {ECO:0000313|RefSeq:NP_001004578.1,
GN ECO:0000313|ZFIN:ZDB-GENE-030131-2069}, wu:fb95h05
GN {ECO:0000313|RefSeq:NP_001004578.1}, zgc:92331
GN {ECO:0000313|RefSeq:NP_001004578.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AAH81648.1};
RN [1] {ECO:0000313|EMBL:AAH81648.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Whole {ECO:0000313|EMBL:AAH81648.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSDARP00000020181}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000020181};
RG Ensembl;
RL Submitted (JUL-2011) to UniProtKB.
RN [3] {ECO:0000313|RefSeq:NP_001004578.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=23720042;
RA Harvey S.A., Sealy I., Kettleborough R., Fenyes F., White R., Stemple D.,
RA Smith J.C.;
RT "Identification of the zebrafish maternal and paternal transcriptomes.";
RL Development 140:2703-2710(2013).
RN [4] {ECO:0000313|RefSeq:NP_001004578.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=23412571; DOI=10.1074/mcp.m111.015594;
RA Konzer A., Ruhs A., Braun H., Jungblut B., Braun T., Kruger M.;
RT "Stable isotope labeling in zebrafish allows in vivo monitoring of cardiac
RT morphogenesis.";
RL Mol. Cell. Proteomics 12:1502-1512(2013).
RN [5] {ECO:0000313|Ensembl:ENSDARP00000020181, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000020181};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [6] {ECO:0000313|RefSeq:NP_001004578.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=25686250; DOI=10.1038/ncb3109;
RA Lu Q., Insinna C., Ott C., Stauffer J., Pintado P.A., Rahajeng J., Baxa U.,
RA Walia V., Cuenca A., Hwang Y.S., Daar I.O., Lopes S.,
RA Lippincott-Schwartz J., Jackson P.K., Caplan S., Westlake C.J.;
RT "Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary
RT vesicle formation.";
RL Nat. Cell Biol. 17:228-240(2015).
RN [7] {ECO:0000313|RefSeq:NP_001004578.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27189481;
RA Pasquier J., Cabau C., Nguyen T., Jouanno E., Severac D., Braasch I.,
RA Journot L., Pontarotti P., Klopp C., Postlethwait J.H., Guiguen Y.,
RA Bobe J.;
RT "Gene evolution and gene expression after whole genome duplication in fish:
RT the PhyloFish database.";
RL BMC Genomics 17:368-368(2016).
RN [8] {ECO:0000313|RefSeq:NP_001004578.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=28252024;
RA Bayes A., Collins M.O., Reig-Viader R., Gou G., Goulding D., Izquierdo A.,
RA Choudhary J.S., Emes R.D., Grant S.G.;
RT "Evolution of complexity in the zebrafish synapse proteome.";
RL Nat. Commun. 8:14613-14613(2017).
RN [9] {ECO:0000313|RefSeq:NP_001004578.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=30683896;
RA Insinna C., Lu Q., Teixeira I., Harned A., Semler E.M., Stauffer J.,
RA Magidson V., Tiwari A., Kenworthy A.K., Narayan K., Westlake C.J.;
RT "Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation
RT function in cilia assembly and transport.";
RL Nat. Commun. 10:428-428(2019).
RN [10] {ECO:0000313|RefSeq:NP_001004578.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004413};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004413};
CC Cytoplasmic side {ECO:0000256|ARBA:ARBA00004413}. Endosome membrane
CC {ECO:0000256|ARBA:ARBA00004125}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004125}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004125}. Membrane
CC {ECO:0000256|ARBA:ARBA00004287}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004287}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004287}.
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DR EMBL; BX248510; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC081648; AAH81648.1; -; mRNA.
DR RefSeq; NP_001004578.1; NM_001004578.1.
DR STRING; 7955.ENSDARP00000020181; -.
DR PaxDb; 7955-ENSDARP00000020181; -.
DR Ensembl; ENSDART00000012717.8; ENSDARP00000020181.7; ENSDARG00000014793.8.
DR Ensembl; ENSDART00000186101.1; ENSDARP00000148348.1; ENSDARG00000110243.1.
DR Ensembl; ENSDART00000186394.1; ENSDARP00000150332.1; ENSDARG00000110919.1.
DR GeneID; 447839; -.
DR KEGG; dre:447839; -.
DR AGR; ZFIN:ZDB-GENE-030131-2069; -.
DR CTD; 447839; -.
DR ZFIN; ZDB-GENE-030131-2069; ehd1b.
DR eggNOG; KOG1954; Eukaryota.
DR HOGENOM; CLU_017595_1_1_1; -.
DR OMA; PANCAKH; -.
DR OrthoDB; 12127at2759; -.
DR TreeFam; TF314429; -.
DR Proteomes; UP000000437; Alternate scaffold 10.
DR Proteomes; UP000000437; Chromosome 10.
DR Bgee; ENSDARG00000014793; Expressed in testis and 28 other cell types or tissues.
DR GO; GO:0060170; C:ciliary membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005769; C:early endosome; IBA:GO_Central.
DR GO; GO:0030139; C:endocytic vesicle; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0055038; C:recycling endosome membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR GO; GO:0060271; P:cilium assembly; IMP:UniProtKB.
DR GO; GO:0032456; P:endocytic recycling; IBA:GO_Central.
DR GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR GO; GO:0072659; P:protein localization to plasma membrane; IBA:GO_Central.
DR GO; GO:0070293; P:renal absorption; IGI:ZFIN.
DR CDD; cd00052; EH; 1.
DR CDD; cd09913; EHD; 1.
DR Gene3D; 1.10.268.20; -; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR040990; DUF5600.
DR InterPro; IPR045063; Dynamin_N.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR000261; EH_dom.
DR InterPro; IPR031692; EHD_N.
DR InterPro; IPR030381; G_DYNAMIN_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11216; EH DOMAIN; 1.
DR PANTHER; PTHR11216:SF161; EPIDERMAL GROWTH FACTOR RECEPTOR PATHWAY SUBSTRATE CLONE 15, ISOFORM A; 1.
DR Pfam; PF18150; DUF5600; 1.
DR Pfam; PF00350; Dynamin_N; 1.
DR Pfam; PF12763; EF-hand_4; 1.
DR Pfam; PF16880; EHD_N; 1.
DR SMART; SM00027; EH; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS50031; EH; 1.
DR PROSITE; PS51718; G_DYNAMIN_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Endosome {ECO:0000256|ARBA:ARBA00022753};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022840};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q66HW2};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437}.
FT DOMAIN 54..285
FT /note="Dynamin-type G"
FT /evidence="ECO:0000259|PROSITE:PS51718"
FT DOMAIN 443..531
FT /note="EH"
FT /evidence="ECO:0000259|PROSITE:PS50031"
FT DOMAIN 475..510
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
SQ SEQUENCE 532 AA; 60007 MW; 06741C1C4E2920C7 CRC64;
MFSWSNKDGK KKDPELFQTV SDGLKRLYRT KLFPLEDAYR FHDFHSPALE DADFDNKPMV
LLVGQYSTGK TTFIRHLMEQ DFPGMRIGPE PTTDSFIAVM HGEQEGLIPG NALVVDPKKP
FRKLNAFGNA FLNRFICAQM PNPVLDSISI IDTPGILSGE KQRISRGYDF AAVLEWFAER
VDRIILLFDA HKLDISDEFS EVIRALKNHE DKMRVVLNKA DQIGTQQLMR VYGALMWSLG
KIVNTPEVIR VYIGSFWAQP LLVADNRKLF EAEEQDLFRD IQGLPRNAAL RKLNDLIKRA
RLAKVQAYII SSLKKEMPNV FGKENKKKEL IANLGEIYAK IEKEHQISPG DFPKLSKMQE
VLAGQDFTKF QGLKPKLLES VEDMLANDIA KLMTLVRQEE ASMPSQAVQG GAFEGTMNGP
FGHGYGEGAG EGIDELEWVV AKDKPTYDEI FYTLSPVNGK VSGAMAKKEM VKSKLPNNVL
GKIWKLADVD KDGYLDDEEF ALANHLIKVK LEGHELPADL PDHLVPPSKR EQ
//
