ID Q71FV0_9INFA Unreviewed; 751 AA.
AC Q71FV0;
DT 05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 79.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035, ECO:0000256|RuleBase:RU004330};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494, ECO:0000256|RuleBase:RU004330};
DE Flags: Fragment;
GN Name=PB1 {ECO:0000313|EMBL:AAQ04923.1};
OS Influenza A virus (A/Chicken/Sichuan/5/97(H9N2)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=197584 {ECO:0000313|EMBL:AAQ04923.1};
RN [1] {ECO:0000313|EMBL:AAQ04923.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/Chicken/Sichuan/5/97 {ECO:0000313|EMBL:AAQ04923.1};
RA Li J.W., Yu K.Z., Brown I., Shortridge K.F., Pieris J.S.M., Guan Y.;
RT "The evolution of H9N2 influenza A viruses isolated from Eurasian
RT countries.";
RL Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|RuleBase:RU004330};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; AF508634; AAQ04923.1; -; Genomic_RNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR Gene3D; 6.10.140.720; -; 1.
DR HAMAP; MF_04065; INFV_RDRP; 1.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PIRSF; PIRSF000827; RdRPol_OMV; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 3: Inferred from homology;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW ECO:0000256|RuleBase:RU004330};
KW Transferase {ECO:0000256|ARBA:ARBA00022484, ECO:0000256|RuleBase:RU004330};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT DOMAIN 279..476
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT REGION 46..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AAQ04923.1"
SQ SEQUENCE 751 AA; 85542 MW; 6AAEF894241A6A81 CRC64;
LFLKVPAQNA ISTTFPYTGD PPYSHGTGTG YTMDTVNRTH QYSEKGRWTT NTETGAPQLN
PIDGPLPEDN EPSGYAQTDC VLEAMAFLEE SHPGLFENSC LETMEVVQQT RVDKLTQGRQ
TYDWTLNRNQ PAATALANTI EVFRSNGLTA NESGRLIDFL KDVMESMDKE EMEITTHFQR
KRRVRDNMTK KMVTQRTIGK KKQRLTKKSY LIRALTLNTM TKDAERGKLK RRAIATPGMQ
IRGFVHFVEA LARSICEKLE QSGLPVGGNE KKAKLANVVR KMMTNSQDTE LSFTVTGDNT
KWNENQNPRI FLAMITYITR NQPEWFRNVL SIAPIMFSNK MARLGKGYMF ESKSMKLRTQ
IPAEMLANID LKYFNESTRK KIEKIRPLLT EGTASLSPGM MMGMFNMLST VLGVSILNLG
QKRYTKTTYW WDGLQSSDDF ALIVNAPNHE GIQAGVDRFY RTCKLVGINM SKKKSYINRT
GTFEFTSFFY RYGFVANFSM ELPSFGVSGI NESADMSIGV TVIKNNMINN DLGPATAQMA
LQLFIKDYRY TYRCHRGDTQ IQTRRSFELR KLWEQTRSKA GLLVSDGGPN LYNIRNLHIP
EVCLKWELMD EDYQGRLCNP LNPFVSHKEV ESVNNAVVMP AHGPAKSMEY DAVATTHSWI
PKRNRSILNT SQRGILEDEQ MYQKCCTLFE KFFPSSSYRR PVGISSMMEA MVSRARIDAR
VDFESGRIKK EEFAEILKIC STIEELGRQG K
//
