ID Q877F7_METMZ Unreviewed; 245 AA.
AC Q877F7;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 22-FEB-2023, entry version 56.
DE RecName: Full=coenzyme-B sulfoethylthiotransferase {ECO:0000256|ARBA:ARBA00013271};
DE EC=2.8.4.1 {ECO:0000256|ARBA:ARBA00013271};
DE Flags: Fragment;
GN Name=mcrA {ECO:0000313|EMBL:AAP20895.1};
OS Methanosarcina mazei (Methanosarcina frisia).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=2209 {ECO:0000313|EMBL:AAP20895.1};
RN [1] {ECO:0000313|EMBL:AAP20895.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=MT {ECO:0000313|EMBL:AAP20895.1};
RX PubMed=12866859; DOI=10.1078/072320203322346173;
RA Simankova M.V., Kotsyurbenko O.R., Lueders T., Nozhevnikova A.N.,
RA Wagner B., Conrad R., Friedrich M.W.;
RT "Isolation and characterization of new strains of methanogens from cold
RT terrestrial habitats.";
RL Syst. Appl. Microbiol. 26:312-318(2003).
RN [2] {ECO:0000313|EMBL:BAF56657.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=TMA {ECO:0000313|EMBL:BAF56657.1};
RA Watanabe T., Kimura M., Asakawa S.;
RT "Distinct members of a stable methanogenic archaeal community transcribe
RT mcrA genes under flooded and drained conditions in Japanese paddy field
RT soil.";
RL Soil Biol. Biochem. 41:276-285(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B
CC heterodisulfide + methane; Xref=Rhea:RHEA:12532, ChEBI:CHEBI:16183,
CC ChEBI:CHEBI:58286, ChEBI:CHEBI:58411, ChEBI:CHEBI:58596; EC=2.8.4.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000951};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12533;
CC Evidence={ECO:0000256|ARBA:ARBA00000951};
CC -!- COFACTOR:
CC Name=coenzyme F430; Xref=ChEBI:CHEBI:60540;
CC Evidence={ECO:0000256|ARBA:ARBA00001952};
CC -!- PATHWAY: One-carbon metabolism; methyl-coenzyme M reduction; methane
CC from methyl-coenzyme M: step 1/1. {ECO:0000256|ARBA:ARBA00005149}.
CC -!- SUBUNIT: MCR is a hexamer of two alpha, two beta, and two gamma chains,
CC forming a dimer of heterotrimers. {ECO:0000256|ARBA:ARBA00011155}.
CC -!- SIMILARITY: Belongs to the methyl-coenzyme M reductase alpha subunit
CC family. {ECO:0000256|ARBA:ARBA00010434}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY260440; AAP20895.1; -; Genomic_DNA.
DR EMBL; AB300778; BAF56657.1; -; Genomic_DNA.
DR AlphaFoldDB; Q877F7; -.
DR UniPathway; UPA00646; UER00699.
DR GO; GO:0050524; F:coenzyme-B sulfoethylthiotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.470; -; 1.
DR Gene3D; 1.20.840.10; Methyl-coenzyme M reductase, alpha/beta subunit, C-terminal; 1.
DR InterPro; IPR008924; Me_CoM_Rdtase_asu/bsu_C.
DR InterPro; IPR009047; Me_CoM_Rdtase_asu_C.
DR InterPro; IPR003183; Me_CoM_Rdtase_asu_N.
DR InterPro; IPR015823; Me_CoM_Rdtase_asu_N_sub2.
DR InterPro; IPR009024; Me_CoM_Rdtase_Fd-like_fold.
DR Pfam; PF02249; MCR_alpha; 1.
DR Pfam; PF02745; MCR_alpha_N; 1.
DR SUPFAM; SSF48081; Methyl-coenzyme M reductase alpha and beta chain C-terminal domain; 1.
DR SUPFAM; SSF55088; Methyl-coenzyme M reductase subunits; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Methanogenesis {ECO:0000256|ARBA:ARBA00022994};
KW Methylation {ECO:0000256|ARBA:ARBA00022481};
KW Nickel {ECO:0000256|ARBA:ARBA00022596}.
FT DOMAIN 1..34
FT /note="Methyl-coenzyme M reductase alpha subunit N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF02745"
FT DOMAIN 81..213
FT /note="Methyl-coenzyme M reductase alpha subunit C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF02249"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AAP20895.1"
FT NON_TER 245
FT /evidence="ECO:0000313|EMBL:AAP20895.1"
SQ SEQUENCE 245 AA; 26020 MW; B9964AADB7A175C5 CRC64;
FISAYAMCAG EAAVADLSFA AKHAALVSMG EMLPARRARG PNEPGGLSFG HLSDIIQTSR
TSQDPAKIAL EVVGAGCMLY DQIWLGSYMS GGVGFTQYAT AAYTDDILDN NVYYDVDYIN
DKYNGAANLG TDNKVKATLD VVKDIATEST LYGIETYEKF PTALEDHFGG SQRATVLAAA
AGVACALGTA NANAGLSGWY LSMYLHKEAW GRLGFFGFDL QDQCGATNVL SYQGDEGLPD
ELRGP
//
