ID Q88EV9_PSEPK Unreviewed; 435 AA.
AC Q88EV9;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 132.
DE RecName: Full=Flagellar biosynthesis protein FlhF {ECO:0000256|ARBA:ARBA00014919};
DE AltName: Full=Flagella-associated GTP-binding protein {ECO:0000256|ARBA:ARBA00030866};
GN Name=flhF {ECO:0000313|EMBL:AAN69922.1};
GN OrderedLocusNames=PP_4343 {ECO:0000313|EMBL:AAN69922.1};
OS Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS / KT2440).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=160488 {ECO:0000313|EMBL:AAN69922.1, ECO:0000313|Proteomes:UP000000556};
RN [1] {ECO:0000313|EMBL:AAN69922.1, ECO:0000313|Proteomes:UP000000556}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440
RC {ECO:0000313|Proteomes:UP000000556};
RX PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA Martins dos Santos V.A., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA Brinkac L., Beanan M., DeBoy R.T., Daugherty S., Kolonay J., Madupu R.,
RA Nelson W., White O., Peterson J., Khouri H., Hance I., Chris Lee P.,
RA Holtzapple E., Scanlan D., Tran K., Moazzez A., Utterback T., Rizzo M.,
RA Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D.,
RA Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N.,
RA Dusterhoft A., Tummler B., Fraser C.M.;
RT "Complete genome sequence and comparative analysis of the metabolically
RT versatile Pseudomonas putida KT2440.";
RL Environ. Microbiol. 4:799-808(2002).
CC -!- FUNCTION: Necessary for flagellar biosynthesis. May be involved in
CC translocation of the flagellum. {ECO:0000256|ARBA:ARBA00025337}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004413};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004413};
CC Cytoplasmic side {ECO:0000256|ARBA:ARBA00004413}. Membrane
CC {ECO:0000256|ARBA:ARBA00004287}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004287}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004287}.
CC -!- SIMILARITY: Belongs to the GTP-binding SRP family.
CC {ECO:0000256|ARBA:ARBA00008531}.
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DR EMBL; AE015451; AAN69922.1; -; Genomic_DNA.
DR RefSeq; NP_746458.1; NC_002947.4.
DR RefSeq; WP_010955071.1; NC_002947.4.
DR AlphaFoldDB; Q88EV9; -.
DR STRING; 160488.PP_4343; -.
DR PaxDb; 160488-PP_4343; -.
DR GeneID; 83678948; -.
DR KEGG; ppu:PP_4343; -.
DR PATRIC; fig|160488.4.peg.4617; -.
DR eggNOG; COG1419; Bacteria.
DR HOGENOM; CLU_009301_11_5_6; -.
DR OrthoDB; 9778554at2; -.
DR PhylomeDB; Q88EV9; -.
DR BioCyc; PPUT160488:G1G01-4621-MONOMER; -.
DR Proteomes; UP000000556; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0044781; P:bacterial-type flagellum organization; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR CDD; cd17873; FlhF; 1.
DR Gene3D; 1.20.120.1380; Flagellar FlhF biosynthesis protein, N domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR020006; FlhF.
DR InterPro; IPR047040; FlhF__GTPase_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000897; SRP54_GTPase_dom.
DR NCBIfam; TIGR03499; FlhF; 1.
DR PANTHER; PTHR43134:SF3; FLAGELLAR BIOSYNTHESIS PROTEIN FLHF; 1.
DR PANTHER; PTHR43134; SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA; 1.
DR Pfam; PF00448; SRP54; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00962; SRP54; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Bacterial flagellum biogenesis {ECO:0000256|ARBA:ARBA00022795};
KW Bacterial flagellum protein export {ECO:0000256|ARBA:ARBA00023225};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cell projection {ECO:0000313|EMBL:AAN69922.1};
KW Cilium {ECO:0000313|EMBL:AAN69922.1};
KW Flagellum {ECO:0000313|EMBL:AAN69922.1};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein transport {ECO:0000256|ARBA:ARBA00022927};
KW Reference proteome {ECO:0000313|Proteomes:UP000000556};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 215..385
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 216..408
FT /note="SRP54-type proteins GTP-binding"
FT /evidence="ECO:0000259|SMART:SM00962"
SQ SEQUENCE 435 AA; 47447 MW; DE4E878ADB78838F CRC64;
MQVKRFFAAD MRQAMKLVRD ELGADAAIIG NRRIAGGVEL TAALDYKLSA LAPRVPNAEL
EEELRKTHTR IATAQAELDQ RQDSSDNNRQ LFAGQSLTAA EPLIEPHVEV PEAAAVPAAA
PVDPRLFDAM RSELSGLREL LEVQLGSLAW NQLQGSKPQQ ANLWRRLQRI GLSGPIAREL
LDLTAEIEEP RQAWRMLLAH LARMIEVPEI EPIEEGGVIA MVGPAGMGKT TTLAKLAARY
VLKYGAQNLA LVSMDSFRIG AQEQLKTLGR ILNVPVTYVD PGQSLAAALE PLLRKRVVLI
DTAGLQASDP ALRMQLETLA GRGIAAKNYL VLATTSQKQV LTAAYHSYKR CGLAGCILTK
LDETASLGDV LSLAISHELP VAYLTDGPRI PDDLHLPRGH QLVTRAVNVQ QQDEPSEEAM
ADMFADLYHN PRRAG
//
