ID   Q8LKZ7_HORVU            Unreviewed;       789 AA.
AC   Q8LKZ7;
DT   01-OCT-2002, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2002, sequence version 1.
DT   27-MAR-2024, entry version 85.
DE   SubName: Full=Isoamylase {ECO:0000313|EMBL:AAM46866.1};
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513 {ECO:0000313|EMBL:AAM46866.1};
RN   [1] {ECO:0000313|EMBL:AAM46866.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=12100486; DOI=10.1046/j.1365-313X.2002.01339.x;
RA   Burton R.A., Jenner H., Carrangis L., Fahy B., Fincher G.B., Hylton C.,
RA   Laurie D.A., Parker M., Waite D., van Wegen S., Verhoeven T., Denyer K.;
RT   "Starch granule initiation and growth are altered in barley mutants that
RT   lack isoamylase activity.";
RL   Plant J. 31:97-112(2002).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000256|ARBA:ARBA00004229}.
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DR   EMBL; AF490375; AAM46866.1; -; mRNA.
DR   AlphaFoldDB; Q8LKZ7; -.
DR   CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   EnsemblPlants; HORVU.MOREX.r2.7HG0565540.1; HORVU.MOREX.r2.7HG0565540.1; HORVU.MOREX.r2.7HG0565540.
DR   EnsemblPlants; HORVU.MOREX.r2.7HG0565540.1.mrna1; HORVU.MOREX.r2.7HG0565540.1.mrna1; HORVU.MOREX.r2.7HG0565540.1.
DR   Gramene; HORVU.MOREX.r2.7HG0565540.1; HORVU.MOREX.r2.7HG0565540.1; HORVU.MOREX.r2.7HG0565540.
DR   Gramene; HORVU.MOREX.r2.7HG0565540.1.mrna1; HORVU.MOREX.r2.7HG0565540.1.mrna1; HORVU.MOREX.r2.7HG0565540.1.
DR   ExpressionAtlas; Q8LKZ7; baseline and differential.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0019156; F:isoamylase activity; IEA:UniProt.
DR   GO; GO:0005983; P:starch catabolic process; IEA:UniProt.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR048650; ISOA1-3-like_C.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF21156; ISOA1-3_C; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW   Plastid {ECO:0000256|ARBA:ARBA00022640};
KW   Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT   DOMAIN          235..664
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   789 AA;  88668 MW;  5A6E6A77E75526A0 CRC64;
     MKMMAMAEAP CLCARPFLAA RAWRPGPAPR LRRWRPNATA GKGVGEVCAA AVEAATKAEE
     EEDEEEEPVA EDRYALGGAC RVLAGMPAPL GATALAGGVN FAVYSGGATA AALCLFTPED
     LKADRVSEEV PLDPLMNRTG DVWHVFLEGE LHGMLYGYRF DGTFAPHCGH YFDVSNVVVD
     PYAKAVISRE EYGVPAHGNN CWPQMAGMIP LPYSTFDWEG DLPLRYPQKD LVIYEMHLRG
     FTKHDSSNVE HPGTFIGAVS KLDYLKELGV NCIELMPCHE FNELEYATSS SKMNFWGYST
     INFFSPMTRY TSGGIKNCGR DGINEFKTFV RESHKRGIEV ILDVVFNHTA EGNENGPILS
     FRGVDNTTYY MLAPKGEFYN YSGCGNTFNC NHPVVRQFIV DCLRYWVMEM HIDGFRFDLA
     SIMTRGSSLW DPINVYGAPI EGDMITTGTP LVTPPLIDMI SNDPILGGVK LIAEAWDAGG
     LYQVGQFPHW NVWSEWNGKY RDIVRQFIKG TDGFAGGFAE CLCGSPQLYQ AGGRKPWHSI
     NFVCAHDGFT LADLVTYNNK YNLPNGEDNR DGENHNLSWN CGEEGEFARS SVKRLRKRQM
     RNFFVCLMVS QGVPMFYMGD EYGHTKGGNN NTYCHDSYVN YFRWDKKEEH SDLHRFCCLM
     TKFRKECEGL GLEDFPTAEQ LQWHGHQPGK PDWSEKSRFV AFSMKDETKG EIYVAFNTSH
     LAAVVELPER IGHRWEPVVD TGKPAPYDFL TDDLPDRALT VQQFSHFLNS NLYPMLSYTS
     VILVSRPDV
//