ID Q8RVW4_SOLLC Unreviewed; 636 AA.
AC Q8RVW4;
DT 01-JUN-2002, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2002, sequence version 1.
DT 22-FEB-2023, entry version 76.
DE RecName: Full=beta-fructofuranosidase {ECO:0000256|ARBA:ARBA00012758};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081 {ECO:0000313|EMBL:AAL75450.1};
RN [1] {ECO:0000313|EMBL:AAL75450.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Mature fruit {ECO:0000313|EMBL:AAL75450.1};
RA Westphal S., Foetisch K., Haustein D., Vieths S., Scheurer S.;
RT "Cloning and characterization of beta-fructofuranosidase, a newly
RT identified glycoprotein as a minor allergen of tomato (Lycopersicon
RT Esculentum).";
RL Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004606}; Single-
CC pass type II membrane protein {ECO:0000256|ARBA:ARBA00004606}. Vacuole
CC lumen {ECO:0000256|ARBA:ARBA00004410}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
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DR EMBL; AF465613; AAL75450.1; -; mRNA.
DR AlphaFoldDB; Q8RVW4; -.
DR Allergome; 704; Sola l 2.
DR Allergome; 706; Sola l 2.0102.
DR CAZy; GH32; Glycoside Hydrolase Family 32.
DR ExpressionAtlas; Q8RVW4; baseline and differential.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005775; C:vacuolar lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18624; GH32_Fruct1-like; 1.
DR InterPro; IPR021792; Beta-fructofuranosidase_N.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR31953:SF84; ACID BETA-FRUCTOFURANOSIDASE; 1.
DR PANTHER; PTHR31953; BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR Pfam; PF11837; INV_N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 2: Evidence at transcript level;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Signal-anchor {ECO:0000256|ARBA:ARBA00022968};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Vacuole {ECO:0000256|ARBA:ARBA00022554}.
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 62..100
FT /note="Beta-fructofuranosidase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF11837"
FT DOMAIN 108..427
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 430..622
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 636 AA; 70073 MW; B848DBB8ADFB036E CRC64;
MATQCYDPEN SASRYTLLPD QPDSGHRKSL KIISGIFLSV FLLLSVAFFP ILNNQSPDLQ
IDSRSPAPPS RGVSQGVSDK TFRDVAGASH VSYAWSNAML SWQRTAYHFQ PQKNWMNDPN
GPLYHKGWYH LFYQYNPDSA IWGNITWGHA VSKDLIHWLY LPFAMVPDQW YDINGVWTGS
ATILPDGQII MLYTGDTDDY VQVQNLAYPA NLSDPLLLDW VKFKANPVLV PPPGIGVKDF
RDPITAWTGP QNGQWLLTIG SKIGKTGVAL VCETSNFTSF KLLDGVLHAV PGTGMWECVD
FYPVSTKKTN GLDTSYNGPG VKHVLKASLD DNKQDHYAIG TYDLGKNKWT PDNPELDCGI
GLRLDYGKYY ASKTFYDPKR ERRVLWGWIG ETDSESADLQ KGWASVQSIP RTVLYDKKTG
THLLQWPVEE IESLRVGDPT VKQVDLQPGS IELLRVDSAA ELDIEASFEV DKVALQGIIE
ADHVGFSCST SGGAASRGIL GPFGVIVIAD QTLSELTPVY FYISKGADGR AETHFCADQT
RSSEAPGVGK QVYGSSVPVL DGEKHSMRLL VDHSIVESFA QGGRTVITSR IYPTKAVNGA
ARLFVFNNAT GASVTASVKI WSLESANIQS FPLQDL
//
