ID Q8TP84_METAC Unreviewed; 755 AA.
AC Q8TP84;
DT 01-JUN-2002, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2002, sequence version 1.
DT 27-MAR-2024, entry version 111.
DE SubName: Full=Nitrogen fixation protein NifH/NifE {ECO:0000313|EMBL:AAM05435.1};
GN OrderedLocusNames=MA_2032 {ECO:0000313|EMBL:AAM05435.1};
OS Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS C2A).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=188937 {ECO:0000313|EMBL:AAM05435.1, ECO:0000313|Proteomes:UP000002487};
RN [1] {ECO:0000313|EMBL:AAM05435.1, ECO:0000313|Proteomes:UP000002487}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A
RC {ECO:0000313|Proteomes:UP000002487};
RX PubMed=11932238; DOI=10.1101/gr.223902;
RA Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA Cann I., Graham D.E., Grahame D.A., Guss A., Hedderich R., Ingram-Smith C.,
RA Kuettner C.H., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B.,
RA Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H.,
RA de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L.,
RA Paulsen I., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H.,
RA Lander E., Metcalf W.W., Birren B.;
RT "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT physiological diversity.";
RL Genome Res. 12:532-542(2002).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the NifH/BchL/ChlL family.
CC {ECO:0000256|ARBA:ARBA00005504, ECO:0000256|RuleBase:RU003688}.
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DR EMBL; AE010299; AAM05435.1; -; Genomic_DNA.
DR RefSeq; WP_011022025.1; NC_003552.1.
DR AlphaFoldDB; Q8TP84; -.
DR STRING; 188937.MA_2032; -.
DR EnsemblBacteria; AAM05435; AAM05435; MA_2032.
DR GeneID; 1473921; -.
DR KEGG; mac:MA_2032; -.
DR HOGENOM; CLU_378090_0_0_2; -.
DR InParanoid; Q8TP84; -.
DR PhylomeDB; Q8TP84; -.
DR Proteomes; UP000002487; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR CDD; cd02040; NifH; 1.
DR Gene3D; 3.40.50.12380; Nitrogenase MoFe cofactor biosynthesis protein NifE, C-terminal; 1.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR030655; NifH/chlL_CS.
DR InterPro; IPR000392; NifH/frxC.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR42864; LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE IRON-SULFUR ATP-BINDING PROTEIN; 1.
DR PANTHER; PTHR42864:SF2; LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE IRON-SULFUR ATP-BINDING PROTEIN; 1.
DR Pfam; PF00142; Fer4_NifH; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR PRINTS; PR00091; NITROGNASEII.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00746; NIFH_FRXC_1; 1.
DR PROSITE; PS00692; NIFH_FRXC_2; 1.
DR PROSITE; PS51026; NIFH_FRXC_3; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|RuleBase:RU003688};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU003688};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU003688};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|RuleBase:RU003688};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU003688};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU003688};
KW Oxidoreductase {ECO:0000256|RuleBase:RU003688};
KW Reference proteome {ECO:0000313|Proteomes:UP000002487}.
FT DOMAIN 341..739
FT /note="Nitrogenase/oxidoreductase component 1"
FT /evidence="ECO:0000259|Pfam:PF00148"
FT REGION 304..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 755 AA; 81730 MW; 13690D27BD60F1D5 CRC64;
MIQIALYGKG GIGKSTVSAN LSAALADLNK RVLQVGCDPK HDSTRLLLGG AVIPTVLDYM
RETPGEAQQL EALVFEGYRS TACVEAGGPK PGIGCAGRGI LSSFEVLKRL GLRASSFDVV
LYDVLGDVVC GGFAVPLRKE YADAVFLVTS GEYMALYAAN NILRGIRNFE DTNPRVAGII
FNSRGFFAEE ERVFAFSRAV GLPVLSSIPR DEVFSQAEKA GKTLAEAFPS SASAGIFREL
AVYVETLEKD RSLLHPANPL GDLELEALVL GRRERVCMKP FTTAPATDEK TGTELCSLCQ
EKGPSAEIPS EIPETKSAGT PEEVPTQSPT PLPSRPPLYG CAFSGAATAT FQVSDAATVL
HGPRSCTHIT ADALACSFLR GGGINAGEVK QVPGLLPTDM EEEDVIFGGI EKLERKIEEA
LSAGWQTVFV VSTCPGGIIG DDVKRATSRV ETRFPGARVI PVPVEGVLTG DFSTGLLEGY
KRVADLIDPS VTPEKGLVNI IGERSFSLRE EEHFRTIEGL IKKLGYGVNC RFLKGADTLS
LRSFKKAEIN MLACDDPETR ALQAYLSDRF GLEFFELPFP VGFRESSLWV KALTARLLPG
EDPSPLLEEQ EELYRAGVVK YAPQLSGKRV LVVSYTEDIG WVLDTIRDLG MEIVKVGISV
SFFGRGSPGL LSCEAFPVER DYTDEKRARD VRILMPDLVL SNYAPSVPED GVHYDTIPFS
PQVGFLSGLE LAKRWSTLLR LPVTEGWKYD GGDES
//
