ID Q8VW71_PHODP Unreviewed; 430 AA.
AC Q8VW71;
DT 01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2002, sequence version 1.
DT 24-JAN-2024, entry version 52.
DE RecName: Full=3-deoxy-D-manno-octulosonic acid transferase {ECO:0000256|ARBA:ARBA00019077, ECO:0000256|RuleBase:RU365103};
DE Short=Kdo transferase {ECO:0000256|RuleBase:RU365103};
DE EC=2.4.99.12 {ECO:0000256|ARBA:ARBA00012621, ECO:0000256|RuleBase:RU365103};
DE AltName: Full=Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase {ECO:0000256|ARBA:ARBA00031445, ECO:0000256|RuleBase:RU365103};
GN ORFNames=PDPJ_1_01182 {ECO:0000313|EMBL:GAW43768.1};
OS Photobacterium damsela subsp. piscicida (causative agent of fish
OS pasteurellosis) (Pasteurella piscicida).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Photobacterium.
OX NCBI_TaxID=38294 {ECO:0000313|EMBL:BAB72028.1};
RN [1] {ECO:0000313|EMBL:BAB72028.1}
RP NUCLEOTIDE SEQUENCE.
RA Rattanachai A., Hirono I., Aoki T.;
RT "LPS biosynthesis protein (Serratia marcescens) homolog.";
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:BAB72028.1}
RP NUCLEOTIDE SEQUENCE.
RA Rattanachai A., Hirono I., Aoki T.;
RT "Molecular cloning and characterization of the region encoding for
RT lipopolysaccharide biosynthesis in Pasteurella piscicida.";
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:GAW43768.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=OT-51443 {ECO:0000313|EMBL:GAW43768.1};
RA Aoki T., Teru Y., Morimoto N., Kono T., Sakai M., Takano T., Hawke J.P.,
RA Fukuda Y., Takeyama H., Hikima J.;
RT "Complete Genome Sequence of Photobacterium damselae subsp. piscicida
RT Strain OT-51443 Isolated from Yellowtail (Seriola quinqueradiata) in
RT Japan.";
RL Genome Announc. 5:e00404-17(2017).
RN [4] {ECO:0000313|Proteomes:UP000196133}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OT-51443 {ECO:0000313|Proteomes:UP000196133};
RA Hikima J., Teru Y., Sakai M., Takano T., Takeyama H., Aoki T.;
RT "Complete genome sequence of fish pathogenic bacteria, Photobacterium
RT damsela subsp. piscicida, strain OT-51443, isolated from yellowtail
RT (Seriola quinqueradiata) in Japan.";
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes
CC the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-
CC Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate
CC precursor of lipid A. {ECO:0000256|RuleBase:RU365103}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=CMP-3-deoxy-beta-D-manno-octulosonate + lipid IVA (E. coli) =
CC alpha-Kdo-(2->6)-lipid IVA (E. coli) + CMP + H(+);
CC Xref=Rhea:RHEA:28066, ChEBI:CHEBI:15378, ChEBI:CHEBI:58603,
CC ChEBI:CHEBI:60364, ChEBI:CHEBI:60377, ChEBI:CHEBI:85987;
CC EC=2.4.99.12; Evidence={ECO:0000256|ARBA:ARBA00034406,
CC ECO:0000256|RuleBase:RU365103};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004713, ECO:0000256|RuleBase:RU365103}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU365103}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC {ECO:0000256|RuleBase:RU365103}.
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DR EMBL; AB074288; BAB72028.1; -; Genomic_DNA.
DR EMBL; BDMQ01000001; GAW43768.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8VW71; -.
DR CAZy; GT30; Glycosyltransferase Family 30.
DR UniPathway; UPA00958; -.
DR Proteomes; UP000196133; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043842; F:Kdo transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.11720; 3-Deoxy-D-manno-octulosonic-acid transferase, N-terminal domain; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR007507; Glycos_transf_N.
DR InterPro; IPR038107; Glycos_transf_N_sf.
DR InterPro; IPR039901; Kdotransferase.
DR PANTHER; PTHR42755:SF1; 3-DEOXY-D-MANNO-OCTULOSONIC ACID TRANSFERASE, MITOCHONDRIAL-RELATED; 1.
DR PANTHER; PTHR42755; 3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR Pfam; PF04413; Glycos_transf_N; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|RuleBase:RU365103};
KW Lipopolysaccharide biosynthesis {ECO:0000256|RuleBase:RU365103};
KW Membrane {ECO:0000256|RuleBase:RU365103};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365103};
KW Transmembrane {ECO:0000256|RuleBase:RU365103};
KW Transmembrane helix {ECO:0000256|RuleBase:RU365103}.
FT TRANSMEM 12..30
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365103"
FT DOMAIN 42..218
FT /note="3-deoxy-D-manno-octulosonic-acid transferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF04413"
FT DOMAIN 253..407
FT /note="Glycosyl transferase family 1"
FT /evidence="ECO:0000259|Pfam:PF00534"
FT ACT_SITE 68
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR639901-1"
FT SITE 137
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR639901-2"
FT SITE 215
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR639901-2"
SQ SEQUENCE 430 AA; 47916 MW; 4096E205C468F7AD CRC64;
MKNDDNFMLF RLLYSLLLAC AAPFLLYSLY KKKPGKPQIG ARWREHFGIT PKINAVAPIW
LHTVSVGESI AAIPVIKALK HAYPNKTIIV TTTTTTGAEQ IAKLGNLVEH RYMPIDFSCC
VQRFINMIEP CALLIMETEL WPNTLATVAK ANIPITVINA RLSERSAHRY QQFSWLFAHI
GPYLNHVLCL HQDDANRFIQ LGIDRDKVAV TGSVKFDIEI HPQVRQQAQQ LRQLLGMERP
IWIAASTHKG EDEQLLDAFK TIKQNIPNAL LILVPRHPER FDSVFTLCQQ YGFVTQRRTQ
DHNGLLNSDV YLGDTMGEML TLMGAADVVF MAGSLVGDAV GGHNMLEPAA LAKPILTGPS
FYNFTDITEQ LEQANGLIIC QNSEEISHNI CQLLSQKALQ QQMGQAALHV VKSNQGAVAK
TVAAIAPYCH
//
