ID Q8Y3U6_LISMO Unreviewed; 560 AA.
AC Q8Y3U6;
DT 01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2002, sequence version 1.
DT 27-MAR-2024, entry version 115.
DE RecName: Full=Sucrose phosphorylase {ECO:0000256|PIRNR:PIRNR003059};
DE EC=2.4.1.7 {ECO:0000256|PIRNR:PIRNR003059};
DE AltName: Full=Sucrose glucosyltransferase {ECO:0000256|PIRNR:PIRNR003059};
GN OrderedLocusNames=lmo2735 {ECO:0000313|EMBL:CAD00948.1};
OS Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e).
OC Bacteria; Bacillota; Bacilli; Bacillales; Listeriaceae; Listeria.
OX NCBI_TaxID=169963 {ECO:0000313|EMBL:CAD00948.1, ECO:0000313|Proteomes:UP000000817};
RN [1] {ECO:0000313|EMBL:CAD00948.1, ECO:0000313|Proteomes:UP000000817}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-679 / EGD-e {ECO:0000313|Proteomes:UP000000817};
RX PubMed=11679669; DOI=10.1126/science.1063447;
RA Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F.,
RA Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A.,
RA Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E.,
RA Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.D.,
RA Fsihi H., Portillo F.G., Garrido P., Gautier L., Goebel W., Gomez-Lopez N.,
RA Hain T., Hauf J., Jackson D., Jones L.M., Kaerst U., Kreft J., Kuhn M.,
RA Kunst F., Kurapkat G., Madueno E., Maitournam A., Vicente J.M., Ng E.,
RA Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.C.,
RA Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A.,
RA Vazquez-Boland J.A., Voss H., Wehland J., Cossart P.;
RT "Comparative genomics of Listeria species.";
RL Science 294:849-852(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=phosphate + sucrose = alpha-D-glucose 1-phosphate + D-
CC fructose; Xref=Rhea:RHEA:24048, ChEBI:CHEBI:17992, ChEBI:CHEBI:37721,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58601; EC=2.4.1.7;
CC Evidence={ECO:0000256|PIRNR:PIRNR003059};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. Sucrose
CC phosphorylase subfamily. {ECO:0000256|PIRNR:PIRNR003059}.
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DR EMBL; AL591984; CAD00948.1; -; Genomic_DNA.
DR PIR; AF1416; AF1416.
DR RefSeq; NP_466257.1; NC_003210.1.
DR RefSeq; WP_010990053.1; NZ_CP023861.1.
DR AlphaFoldDB; Q8Y3U6; -.
DR STRING; 169963.gene:17595452; -.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR PaxDb; 169963-lmo2735; -.
DR EnsemblBacteria; CAD00948; CAD00948; CAD00948.
DR GeneID; 987075; -.
DR KEGG; lmo:lmo2735; -.
DR PATRIC; fig|169963.11.peg.2801; -.
DR eggNOG; COG0366; Bacteria.
DR HOGENOM; CLU_021358_0_0_9; -.
DR OrthoDB; 9805159at2; -.
DR PhylomeDB; Q8Y3U6; -.
DR BioCyc; LMON169963:LMO2735-MONOMER; -.
DR Proteomes; UP000000817; Chromosome.
DR GO; GO:0009018; F:sucrose phosphorylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11356; AmyAc_Sucrose_phosphorylase-like_1; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR033746; GGa_phosphorylase.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045857; O16G_dom_2.
DR InterPro; IPR016377; Sucrose_GGa_phosphorylase-rel.
DR PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR PANTHER; PTHR10357:SF214; GLUCOSYLGLYCERATE PHOSPHORYLASE; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF003059; Sucrose_phosphorylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|PIRNR:PIRNR003059};
KW Reference proteome {ECO:0000313|Proteomes:UP000000817};
KW Transferase {ECO:0000256|PIRNR:PIRNR003059}.
FT DOMAIN 51..480
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT BINDING 98
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 136
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 226..228
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 336..337
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 444
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
SQ SEQUENCE 560 AA; 63933 MW; 0EB91E0E2F50F580 CRC64;
MQTELVNQIE VKLRKIYQAA YQPAYLEKML ACAENYSNNT RGSIDTISEK NVYLIAYGDS
IFEKNKHPLQ TLNEFLQEYA QDAITDVHLL PIFPSTSDDG FSVTDYKQID EQLGDWDDVQ
KMSENFRVML DFVANHMSKS SDWFKRFSDN EAPYNQFFIE KDSQFDYKNV TRPRTSPLFH
KYENGKELWT TFSEDQLDLN VRNIDCLVAL TDVLLFYASK QATSIRLDAI GFLWKTSGTT
CMHLPETHEI ISLWRLLIDE LYPNLQIITE TNVPHEENIS YFGDGKNEAN MVYQFPLPPL
VLHTFTCHDT TKLSKWAKSI SQVSDTATYF NFLASHDGIG MRPATGILSD EEINSLVQKA
VQNGGQVSYK DNADGTQSVY ELNINYGEAL QNLDEDTTEE LVTKKIIAAH SILLTLQGVP
AIYYHSLLGS KNDLVGYEES GINRRINREK LEKNQLVHEL KTDTYRKTIF TSLKKLVQIR
RNHTAFSPFA TQEILDLGPD VFAIKRESEG ECIYGIINVT SHDISKKVAF SGTNLLANQP
VTSELELTAY EVVWIKKAVQ
//
