ID Q8YB41_BRUME Unreviewed; 294 AA.
AC Q8YB41;
DT 01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2002, sequence version 1.
DT 27-MAR-2024, entry version 115.
DE SubName: Full=2,5-diketo-d-gluconic acid reductase {ECO:0000313|EMBL:AAL54302.1};
DE EC=1.1.1.- {ECO:0000313|EMBL:AAL54302.1};
GN OrderedLocusNames=BMEII1060 {ECO:0000313|EMBL:AAL54302.1};
OS Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=224914 {ECO:0000313|EMBL:AAL54302.1, ECO:0000313|Proteomes:UP000000419};
RN [1] {ECO:0000313|EMBL:AAL54302.1, ECO:0000313|Proteomes:UP000000419}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=16M / ATCC 23456 / NCTC 10094
RC {ECO:0000313|Proteomes:UP000000419};
RX PubMed=11756688; DOI=10.1073/pnas.221575398;
RA DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.J.,
RA Haselkorn R., Kyrpides N., Overbeek R.;
RT "The genome sequence of the facultative intracellular pathogen Brucella
RT melitensis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
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DR EMBL; AE008918; AAL54302.1; -; Genomic_DNA.
DR PIR; AC3642; AC3642.
DR AlphaFoldDB; Q8YB41; -.
DR SMR; Q8YB41; -.
DR KEGG; bme:BMEII1060; -.
DR eggNOG; COG0656; Bacteria.
DR Proteomes; UP000000419; Chromosome II.
DR GO; GO:0050580; F:2,5-didehydrogluconate reductase activity; IEA:InterPro.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR CDD; cd19131; AKR_AKR5C2; 1.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR044504; AKR5C2.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 2.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE 4: Predicted;
KW Oxidoreductase {ECO:0000313|EMBL:AAL54302.1}.
FT DOMAIN 36..278
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
FT ACT_SITE 69
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT BINDING 127
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT SITE 94
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ SEQUENCE 294 AA; 32841 MW; F2D7195E42146D6E CRC64;
MWWPAPRKTA QKISGKGYIM TVPTVKLNDG NHIPQLGYGV WQISNDEAVS AVSEALKAGY
RHIDTATIYG NEEGVGKAIN GSGIARADIF LTTKLWNSDQ GYESTLKAFD TSLKKLGTDY
VDLYLIHWPM PSKDLFMETW RAFIKLKEEG RVKSIGVSNF RTADLERLIK ESGVTPVLNQ
IELHPQFQQD ELRLFHGKHD IATEAWSPLG QGKLLEDPTL KSIAEKHAKS VAQIILRWHI
ETGNIVIPKS ITPARIKENF DIFDFTLNGT DHDAITKLDM ADGRIGPNPS VFSA
//
