ID Q9FFU2_ARATH Unreviewed; 394 AA.
AC Q9FFU2;
DT 01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 134.
DE RecName: Full=tRNA-uridine aminocarboxypropyltransferase {ECO:0000256|ARBA:ARBA00012386};
DE EC=2.5.1.25 {ECO:0000256|ARBA:ARBA00012386};
GN Name=MBG8.15 {ECO:0000313|EMBL:AED96552.1};
GN Synonyms=MBG8_15 {ECO:0000313|EMBL:AED96552.1};
GN OrderedLocusNames=At5g54880 {ECO:0000313|Araport:AT5G54880,
GN ECO:0000313|EMBL:AED96552.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000313|EMBL:BAB08767.1};
RN [1] {ECO:0000313|EMBL:BAB08767.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2] {ECO:0000313|EMBL:AED96552.1, ECO:0000313|Proteomes:UP000006548}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=11130714; DOI=10.1038/35048507;
RG Kazusa DNA Research Institute;
RG Cold Spring Harbor and Washington University in St Louis Sequencing Consortium;
RG European Union Arabidopsis Genome Sequencing Consortium;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., See L.H., Vil D., Baker J.,
RA Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Dusterhoft A., Stiekema W., Pohl T., Entian K.D.,
RA Terryn N., Hartley N., Bent E., Johnson S., Langham S.A., McCullagh B.,
RA Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H.,
RA Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Lankhorst R.K., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S.,
RA Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J.,
RA Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K., Rudd S., Schoof H.,
RA Schueller C., Zaccaria P., Mewes H.W., Bevan M., Fransz P.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3] {ECO:0000313|EMBL:AAK96566.1}
RP NUCLEOTIDE SEQUENCE.
RA Cheuk R., Chen H., Kim C.J., Koesema E., Meyers M.C., Banh J., Bowser L.,
RA Carninci P., Dale J.M., Goldsmith A.D., Hayashizaki Y., Ishida J.,
RA Jiang P.X., Jones T., Kamiya A., Karlin-Neumann G., Kawai J., Lam B.,
RA Lee J.M., Lin J., Liu S.X., Miranda M., Narusaka M., Nguyen M.,
RA Onodera C.S., Palm C.J., Pham P.K., Quach H.L., Sakurai T., Satou M.,
RA Seki M., Southwick A., Tang C.C., Toriumi M., Yamada K., Yamamura Y.,
RA Yu G., Yu S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT "Arabidopsis cDNA clones.";
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:AAN64536.1}
RP NUCLEOTIDE SEQUENCE.
RA Shinn P., Chen H., Cheuk R., Kim C.J., Bowser L., Carninci P., Chan M.M.,
RA Chang C.H., Dale J.M., Hayashizaki Y., Hsuan V.W., Ishida J., Jones T.,
RA Kamiya A., Karlin-Neumann G., Kawai J., Lam B., Lee J.M., Lin J.,
RA Miranda M., Narusaka M., Nguyen M., Palm C.J., Quach H.L., Sakurai T.,
RA Satou M., Seki M., Southwick A., Tang C.C., Toriumi M., Wallender E.K.,
RA Wong C., Wu H.C., Yamada K., Yu G., Yuan S., Shinozaki K., Davis R.W.,
RA Theologis A., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:AED96552.1}
RP NUCLEOTIDE SEQUENCE.
RG TAIR;
RA Swarbreck D., Lamesch P., Wilks C., Huala E.;
RL Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|EMBL:AED96552.1}
RP NUCLEOTIDE SEQUENCE.
RA Krishnakumar V., Cheng C.-Y., Chan A.P., Schobel S., Kim M., Ferlanti E.S.,
RA Belyaeva I., Rosen B.D., Micklem G., Miller J.R., Vaughn M., Town C.D.;
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|Proteomes:UP000006548}
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-
CC amino-3-carboxypropyl]uridine in tRNA + H(+) + S-methyl-5'-
CC thioadenosine; Xref=Rhea:RHEA:62432, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC COMP:16092, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:82930; EC=2.5.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00024168};
CC -!- SIMILARITY: Belongs to the TDD superfamily. DTWD2 family.
CC {ECO:0000256|ARBA:ARBA00034489}.
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DR EMBL; AY052662; AAK96566.1; -; mRNA.
DR EMBL; BT001145; AAN64536.1; -; mRNA.
DR EMBL; CP002688; AED96552.1; -; Genomic_DNA.
DR EMBL; AB005232; BAB08767.1; -; Genomic_DNA.
DR RefSeq; NP_568814.2; NM_124870.4.
DR AlphaFoldDB; Q9FFU2; -.
DR STRING; 3702.Q9FFU2; -.
DR PaxDb; 3702-AT5G54880-1; -.
DR ProteomicsDB; 187005; -.
DR EnsemblPlants; AT5G54880.1; AT5G54880.1; AT5G54880.
DR GeneID; 835579; -.
DR Gramene; AT5G54880.1; AT5G54880.1; AT5G54880.
DR KEGG; ath:AT5G54880; -.
DR Araport; AT5G54880; -.
DR TAIR; AT5G54880; -.
DR eggNOG; KOG4382; Eukaryota.
DR HOGENOM; CLU_040856_0_0_1; -.
DR OMA; RCKEERL; -.
DR OrthoDB; 197144at2759; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FFU2; baseline and differential.
DR GO; GO:0016432; F:tRNA-uridine aminocarboxypropyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006400; P:tRNA modification; IDA:TAIR.
DR InterPro; IPR005636; DTW.
DR InterPro; IPR039262; DTWD2/TAPT.
DR PANTHER; PTHR21392:SF0; TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2; 1.
DR PANTHER; PTHR21392; UNCHARACTERIZED; 1.
DR Pfam; PF03942; DTW; 2.
DR SMART; SM01144; DTW; 1.
PE 1: Evidence at protein level;
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q9FFU2,
KW ECO:0007829|ProteomicsDB:Q9FFU2};
KW Reference proteome {ECO:0000313|Proteomes:UP000006548};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 44..387
FT /note="DTW"
FT /evidence="ECO:0000259|SMART:SM01144"
SQ SEQUENCE 394 AA; 44443 MW; D06B8977B68994CF CRC64;
MLSSGQMMGH VVARTQMILS RSNFADCIRC QGVRVSKTLK MGTKRPTCPS CDKPSQLCLC
KKMRSPCFDN QVSITILQHS LERKHALNST RIARLGLKNV GVTTVFDVHD EAEFLIRVIG
SGCSKIDTNH LDSEYRVENV ASLELDDSFK LGSSGNVENL ESEKNVVLVD HGSLEIAESL
NLSQHLSEIS YRDSRVRDKI GSYEEDLIKI CMKKHGVISN VSHSLMLETN VKVLSFDHIL
ASPAAMDVLA KGFMVTKFSE GKQEFELEVP PGSALLFPSE ESVKINYLKE KEELKVRNLI
VLDGTWSKAR RIYLENPWLK LLRCHVKLEI EGTSLYKEVR RQPRAGCLST IESIVYAMKE
IGEDPEGLDN MLNVFESMVE DQRRCKDENF NKII
//
