ID Q9I072_PSEAE Unreviewed; 341 AA.
AC Q9I072;
DT 01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 111.
DE RecName: Full=diguanylate cyclase {ECO:0000256|ARBA:ARBA00012528};
DE EC=2.7.7.65 {ECO:0000256|ARBA:ARBA00012528};
GN OrderedLocusNames=PA2771 {ECO:0000313|EMBL:AAG06159.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964 {ECO:0000313|EMBL:AAG06159.1, ECO:0000313|Proteomes:UP000002438};
RN [1] {ECO:0000313|EMBL:AAG06159.1, ECO:0000313|Proteomes:UP000002438}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 /
RC 1C / PRS 101 / PAO1 {ECO:0000313|Proteomes:UP000002438};
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.Q., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R., Smith K.,
RA Spencer D., Wong G.K., Wu Z., Paulsen I.T., Reizer J., Saier M.H.,
RA Hancock R.E., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2] {ECO:0007829|PDB:4ZMU}
RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS).
RA Chen Y., Liu C., Liu S., Chi K., Gu L.;
RT "crystal structure of Dcsbis from Pseudomonas aeruginosa.";
RL Submitted (MAY-2015) to the PDB data bank.
RN [3] {ECO:0007829|PDB:4ZMM}
RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 123-341 IN COMPLEX WITH CYCLIC
RP DIGUANOSINE MONOPHOSPHATE.
RA Chen Y., Liu C., Liu S., Chi K., Gu L.;
RT "Crystal structure of Dcsbis GGDEF domain complexed with c-di-GMP.";
RL Submitted (MAY-2015) to the PDB data bank.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE004091; AAG06159.1; -; Genomic_DNA.
DR PIR; B83298; B83298.
DR RefSeq; NP_251461.1; NC_002516.2.
DR RefSeq; WP_003114426.1; NZ_QZGE01000011.1.
DR PDB; 4ZMM; X-ray; 2.50 A; A/B=123-341.
DR PDB; 4ZMU; X-ray; 2.50 A; A/B/C/D=1-341.
DR PDBsum; 4ZMM; -.
DR PDBsum; 4ZMU; -.
DR AlphaFoldDB; Q9I072; -.
DR SMR; Q9I072; -.
DR STRING; 208964.PA2771; -.
DR PaxDb; 208964-PA2771; -.
DR DNASU; 882750; -.
DR GeneID; 882750; -.
DR KEGG; pae:PA2771; -.
DR PATRIC; fig|208964.12.peg.2908; -.
DR PseudoCAP; PA2771; -.
DR HOGENOM; CLU_000445_11_32_6; -.
DR InParanoid; Q9I072; -.
DR OrthoDB; 9812358at2; -.
DR PhylomeDB; Q9I072; -.
DR BioCyc; PAER208964:G1FZ6-2817-MONOMER; -.
DR BRENDA; 2.7.7.65; 5087.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0052621; F:diguanylate cyclase activity; IDA:PseudoCAP.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR CDD; cd01949; GGDEF; 1.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR000160; GGDEF_dom.
DR InterPro; IPR029787; Nucleotide_cyclase.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR NCBIfam; TIGR00254; GGDEF; 1.
DR PANTHER; PTHR45138:SF9; DIGUANYLATE CYCLASE DGCM-RELATED; 1.
DR PANTHER; PTHR45138; REGULATORY COMPONENTS OF SENSORY TRANSDUCTION SYSTEM; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF00990; GGDEF; 1.
DR SMART; SM00065; GAF; 1.
DR SMART; SM00267; GGDEF; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF55073; Nucleotide cyclase; 1.
DR PROSITE; PS50887; GGDEF; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:4ZMM, ECO:0007829|PDB:4ZMU};
KW Nucleotide-binding {ECO:0007829|PDB:4ZMM};
KW Reference proteome {ECO:0000313|Proteomes:UP000002438}.
FT DOMAIN 211..341
FT /note="GGDEF"
FT /evidence="ECO:0000259|PROSITE:PS50887"
FT BINDING 251
FT /ligand="3',3'-c-di-GMP"
FT /ligand_id="ChEBI:CHEBI:58805"
FT /evidence="ECO:0007829|PDB:4ZMM"
FT BINDING 254
FT /ligand="3',3'-c-di-GMP"
FT /ligand_id="ChEBI:CHEBI:58805"
FT /evidence="ECO:0007829|PDB:4ZMM"
FT BINDING 269
FT /ligand="3',3'-c-di-GMP"
FT /ligand_id="ChEBI:CHEBI:58805"
FT /evidence="ECO:0007829|PDB:4ZMM"
FT BINDING 271
FT /ligand="3',3'-c-di-GMP"
FT /ligand_id="ChEBI:CHEBI:58805"
FT /evidence="ECO:0007829|PDB:4ZMM"
FT BINDING 282
FT /ligand="3',3'-c-di-GMP"
FT /ligand_id="ChEBI:CHEBI:58805"
FT /evidence="ECO:0007829|PDB:4ZMM"
SQ SEQUENCE 341 AA; 38032 MW; 0AF98BB343B75774 CRC64;
MLACPLPPDE ALRQQALDDM ALVDTPAEHY LDALVELARE TFGVKTVLIS LIDHDRQWFK
ARIGLDAEQT PRDLSFCGHA ILASEPLMVT DASRDPRFHD NPLVTGPPFI RFYAGEPLHA
SNGQAIGTLC LIDPSPRLLD LREGRQLNRL SILAEGYLQL RSLTEHTRFL RQEIDREQRK
SLLDPLTQLW NRAGFHALHQ HELELARASD QRIGIIYSDI DHFKRINDTL GHRAGDSVLR
EAASRLRAAL RPEDLLARFG GEEFVAMVRV RETTELTMIA NRIRELMEAT PIDCAGTSVP
VTISAGCTLA GSGEEPERAL ARADAALYDA KRAGRNRVVS V
//
