ID Q9I1R7_PSEAE Unreviewed; 531 AA.
AC Q9I1R7;
DT 01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 99.
DE RecName: Full=cyclic-guanylate-specific phosphodiesterase {ECO:0000256|ARBA:ARBA00012282};
DE EC=3.1.4.52 {ECO:0000256|ARBA:ARBA00012282};
GN OrderedLocusNames=PA2200 {ECO:0000313|EMBL:AAG05588.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964 {ECO:0000313|EMBL:AAG05588.1, ECO:0000313|Proteomes:UP000002438};
RN [1] {ECO:0000313|EMBL:AAG05588.1, ECO:0000313|Proteomes:UP000002438}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 /
RC 1C / PRS 101 / PAO1 {ECO:0000313|Proteomes:UP000002438};
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.Q., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R., Smith K.,
RA Spencer D., Wong G.K., Wu Z., Paulsen I.T., Reizer J., Saier M.H.,
RA Hancock R.E., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3',3'-c-di-GMP + H2O = 5'-phosphoguanylyl(3'->5')guanosine +
CC H(+); Xref=Rhea:RHEA:24902, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58754, ChEBI:CHEBI:58805; EC=3.1.4.52;
CC Evidence={ECO:0000256|ARBA:ARBA00034290};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; AE004091; AAG05588.1; -; Genomic_DNA.
DR PIR; E83371; E83371.
DR RefSeq; NP_250890.1; NC_002516.2.
DR RefSeq; WP_003113692.1; NZ_QZGE01000014.1.
DR AlphaFoldDB; Q9I1R7; -.
DR SMR; Q9I1R7; -.
DR STRING; 208964.PA2200; -.
DR PaxDb; 208964-PA2200; -.
DR GeneID; 881519; -.
DR KEGG; pae:PA2200; -.
DR PATRIC; fig|208964.12.peg.2304; -.
DR PseudoCAP; PA2200; -.
DR HOGENOM; CLU_000445_131_1_6; -.
DR InParanoid; Q9I1R7; -.
DR OrthoDB; 9812358at2; -.
DR BioCyc; PAER208964:G1FZ6-2240-MONOMER; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0071111; F:cyclic-guanylate-specific phosphodiesterase activity; IDA:PseudoCAP.
DR CDD; cd01948; EAL; 1.
DR Gene3D; 3.20.20.450; EAL domain; 1.
DR InterPro; IPR024744; CSS-motif_dom.
DR InterPro; IPR001633; EAL_dom.
DR InterPro; IPR035919; EAL_sf.
DR PANTHER; PTHR33121; CYCLIC DI-GMP PHOSPHODIESTERASE PDEF; 1.
DR PANTHER; PTHR33121:SF71; CYCLIC DI-GMP PHOSPHODIESTERASE PDEF; 1.
DR Pfam; PF12792; CSS-motif; 1.
DR Pfam; PF00563; EAL; 1.
DR SMART; SM00052; EAL; 1.
DR SUPFAM; SSF141868; EAL domain-like; 1.
DR PROSITE; PS50883; EAL; 1.
PE 4: Predicted;
KW c-di-GMP {ECO:0000256|ARBA:ARBA00022636};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000002438};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 20..39
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 248..273
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 275..528
FT /note="EAL"
FT /evidence="ECO:0000259|PROSITE:PS50883"
SQ SEQUENCE 531 AA; 58272 MW; 7AB8C94FB1B55B66 CRC64;
MSERGTAAEG RINWRRILPI LLTTTLVGGV CGIFVINELD RQHDGAHLRS YTERALERAL
EISSAADQML REMNAGSAPA CSDRDLEMLR SIVLESRFMS DVGRISGDRV MCTGVRGRLR
APWGLSAPDR VDAAGTRFWS EGMPLPLQAL PTGLVSRGNA VVFIAPHAYF DVDHPAPGIS
AAAVSSGGDY IYHRFAWDDD QPLEALGRPG WPRFIGARQI SLCDPTQDIC VVSLLRERGL
LGTEPGRAAM AAAIGGGLLF GVMLLLSYLF GFLNDEKLPA RLLRALKREA LEVHYQPIVR
LASGRCAGVE ALARWTDSSL GRVSPDVFIG ALEESGDIEL LTRFVFRRAL KQLGPLLREQ
RSFYVSVNVT GKDIADPGFI DFAMRQMARE SVRPEQVALE LTERTTEAQG CLLAGMNRLR
ELGLKIYVDD FGTGHSNLVY LANLPVDAIK IDKVFTQSIG DSSAVELIFD KLCSMAEHLE
IGVVVEGIET QAQADHVLRR SPEALGQGWH FGKALPLEAF LDYYRNQPAG R
//
