ID Q9JP70_ECOLX Unreviewed; 307 AA.
AC Q9JP70;
DT 01-OCT-2000, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2000, sequence version 1.
DT 27-MAR-2024, entry version 83.
DE SubName: Full=EcoVIII endonuclease {ECO:0000313|EMBL:AAF71176.1};
DE SubName: Full=HindIII family type II restriction endonuclease {ECO:0000313|EMBL:EFB2195395.1};
DE SubName: Full=Type II restriction endonuclease EcoVIII {ECO:0000313|EMBL:AET10450.1};
GN Name=ecoVIIIR {ECO:0000313|EMBL:AAF71176.1};
GN ORFNames=EPS97_23750 {ECO:0000313|EMBL:RXB20252.1}, FIJ20_25145
GN {ECO:0000313|EMBL:EFB2195395.1}, G8C01_24435
GN {ECO:0000313|EMBL:MBE9994431.1};
OS Escherichia coli.
OG Plasmid pEC156 {ECO:0000313|EMBL:AAF71176.1}, and
OG Plasmid pEC886 {ECO:0000313|EMBL:AET10450.1}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562 {ECO:0000313|EMBL:AAF71176.1};
RN [1] {ECO:0000313|EMBL:AAF71176.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=E1585-68 {ECO:0000313|EMBL:AAF71176.1};
RC PLASMID=pEC156 {ECO:0000313|EMBL:AAF71176.1};
RX PubMed=9858694; DOI=10.1016/S0378-1119(98)00241-8;
RA Kaczorowski T., Szybalski W.;
RT "Genomic DNA sequencing by SPEL-6 primer walking using hexamer ligation.";
RL Gene 223:83-91(1998).
RN [2] {ECO:0000313|EMBL:AAF71176.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=886 {ECO:0000313|EMBL:AET10450.1}, and E1585-68
RC {ECO:0000313|EMBL:AAF71176.1};
RC PLASMID=pEC156 {ECO:0000313|EMBL:AAF71176.1}, and pEC886
RC {ECO:0000313|EMBL:AET10450.1};
RX PubMed=11591138; DOI=10.1006/plas.2001.1534;
RA Mruk I., Sektas M., Kaczorowski T.;
RT "Characterization of pEC156, a ColE1-type plasmid from Escherichia coli
RT E1585-68 that carries genes of the EcoVIII restriction-modification
RT system.";
RL Plasmid 46:128-139(2001).
RN [3] {ECO:0000313|EMBL:AAF71176.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=E1585-68 {ECO:0000313|EMBL:AAF71176.1};
RC PLASMID=pEC156 {ECO:0000313|EMBL:AAF71176.1};
RX PubMed=12732532; DOI=10.1128/AEM.69.5.2638-2650.2003;
RA Mruk I., Kaczorowski T.;
RT "Genetic organization and molecular analysis of the EcoVIII restriction-
RT modification system of Escherichia coli E1585-68 and its comparison with
RT isospecific homologs.";
RL Appl. Environ. Microbiol. 69:2638-2650(2003).
RN [4] {ECO:0000313|EMBL:AAF71176.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=E1585-68 {ECO:0000313|EMBL:AAF71176.1};
RC PLASMID=pEC156 {ECO:0000313|EMBL:AAF71176.1};
RX PubMed=17468281; DOI=10.1128/AEM.00119-07;
RA Mruk I., Kaczorowski T.;
RT "A rapid and efficient method for cloning genes of type II restriction-
RT modification systems by use of a killer plasmid.";
RL Appl. Environ. Microbiol. 73:4286-4293(2007).
RN [5] {ECO:0000313|EMBL:AET10450.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=886 {ECO:0000313|EMBL:AET10450.1};
RC PLASMID=pEC886 {ECO:0000313|EMBL:AET10450.1};
RA Dekowska A., Kaczorowska A.-K., Kaczorowski T.;
RT "Escherichia coli plasmid pEC886 carrying genes encoding EcoVIII
RT restriction-modification system and transposon confering ampicilin
RT resistance, complete sequence.";
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|EMBL:RXB20252.1, ECO:0000313|Proteomes:UP000290652}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=49_rectal {ECO:0000313|EMBL:RXB20252.1,
RC ECO:0000313|Proteomes:UP000290652};
RA Potter R., Zou Z., Henderson J., Dantas G.;
RT "Genomic analysis of febrile catheter-associated UTI E. coli isolates.";
RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|EMBL:EFB2195395.1, ECO:0000313|Proteomes:UP000519859}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FSIS11921886 {ECO:0000313|EMBL:EFB2195395.1,
RC ECO:0000313|Proteomes:UP000519859};
RG NARMS: The National Antimicrobial Resistance Monitoring System;
RL Submitted (JUN-2019) to the EMBL/GenBank/DDBJ databases.
RN [8] {ECO:0000313|EMBL:MBE9994431.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=6D1-6 {ECO:0000313|EMBL:MBE9994431.1};
RA Fatoba D., Amoako D.G., Abia Akebe L.K., Essack S.;
RT "Genomic Epidemiology of Antibiotic-Resistant Bacteria from Agricultural
RT Soil Fertilized with Poultry Manure in KwaZulu Natal, South Africa.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; AF158026; AAF71176.1; -; Genomic_DNA.
DR EMBL; HQ659758; AET10450.1; -; Genomic_DNA.
DR EMBL; AASDFP010000119; EFB2195395.1; -; Genomic_DNA.
DR EMBL; JAAMTB010000060; MBE9994431.1; -; Genomic_DNA.
DR EMBL; SCIU01000105; RXB20252.1; -; Genomic_DNA.
DR RefSeq; WP_015059042.1; NZ_NSBF01000006.1.
DR RefSeq; YP_006953593.1; NC_019079.1.
DR REBASE; 1003; EcoVIII.
DR REBASE; 259093; EcoM154ORF26685P.
DR REBASE; 263167; EcoSP4ORF25090P.
DR REBASE; 46755; Eco886ORFAP.
DR Proteomes; UP000290652; Unassembled WGS sequence.
DR Proteomes; UP000519859; Unassembled WGS sequence.
DR Proteomes; UP000701764; Unassembled WGS sequence.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR Gene3D; 6.10.250.1510; -; 1.
DR Gene3D; 3.40.91.70; Type II restriction endonuclease, HindIII; 1.
DR InterPro; IPR019043; Restrct_endonuc_II_HindIII.
DR InterPro; IPR038373; Restrct_endonuc_II_HindIII_sf.
DR Pfam; PF09518; RE_HindIII; 1.
PE 4: Predicted;
KW Endonuclease {ECO:0000313|EMBL:AAF71176.1};
KW Hydrolase {ECO:0000313|EMBL:AAF71176.1};
KW Nuclease {ECO:0000313|EMBL:AAF71176.1};
KW Plasmid {ECO:0000313|EMBL:AAF71176.1}.
SQ SEQUENCE 307 AA; 35556 MW; FF9CCBC8890A9B63 CRC64;
MDLTNIEFIN DAAINRRRYW TNEIVKLSGH FVNDSSRVEE EIIYEVSKSG SQALLDHLRL
CTAIPESYEH DSSEEKLYSK YTDALISECF KFFGLNSIVL TERADAADVE VVCDSYSFVA
DAKVFRLSRT AKNQKDFKVQ AMDGWRNTKD FAMVVCPIYQ LPVKSSQIYQ QAILRNVCVF
TYTHLAVLVK YADIVGSDVR ILLEEIFRSV ILMNPSKDSV QYWTMINRTM LNYDKRIEKL
WIDERIATSE GIYVLKKMAI EYLSSERGRI LSMSREEAVR ALIKMNKIES RIEQIKKVTD
NNILSLK
//
