ID UFL1_ARATH Reviewed; 804 AA.
AC Q9LX73;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 24-JAN-2024, entry version 100.
DE RecName: Full=E3 UFM1-protein ligase 1 homolog;
DE EC=2.3.2.-;
DE AltName: Full=E3 UFM1-protein transferase 1 homolog {ECO:0000305};
GN OrderedLocusNames=At3g46220; ORFNames=F12M12.190;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: E3 UFM1-protein ligase that mediates ufmylation of target
CC proteins. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9LX73-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the UFL1 family. {ECO:0000305}.
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DR EMBL; AL355775; CAB90949.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78135.1; -; Genomic_DNA.
DR PIR; T49263; T49263.
DR RefSeq; NP_566883.4; NM_114491.7. [Q9LX73-1]
DR AlphaFoldDB; Q9LX73; -.
DR STRING; 3702.Q9LX73; -.
DR iPTMnet; Q9LX73; -.
DR PaxDb; 3702-AT3G46220-2; -.
DR ProteomicsDB; 245292; -. [Q9LX73-1]
DR EnsemblPlants; AT3G46220.1; AT3G46220.1; AT3G46220. [Q9LX73-1]
DR GeneID; 823767; -.
DR Gramene; AT3G46220.1; AT3G46220.1; AT3G46220. [Q9LX73-1]
DR KEGG; ath:AT3G46220; -.
DR Araport; AT3G46220; -.
DR TAIR; AT3G46220; -.
DR eggNOG; KOG2235; Eukaryota.
DR InParanoid; Q9LX73; -.
DR PhylomeDB; Q9LX73; -.
DR PRO; PR:Q9LX73; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LX73; baseline and differential.
DR Genevisible; Q9LX73; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0061666; F:UFM1 ligase activity; IEA:InterPro.
DR GO; GO:0071568; F:UFM1 transferase activity; IBA:GO_Central.
DR GO; GO:0071569; P:protein ufmylation; IEA:InterPro.
DR GO; GO:0032434; P:regulation of proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IBA:GO_Central.
DR InterPro; IPR018611; Ufl1.
DR PANTHER; PTHR31057; E3 UFM1-PROTEIN LIGASE 1; 1.
DR PANTHER; PTHR31057:SF0; E3 UFM1-PROTEIN LIGASE 1; 1.
DR Pfam; PF09743; E3_UFM1_ligase; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..804
FT /note="E3 UFM1-protein ligase 1 homolog"
FT /id="PRO_0000391894"
FT REGION 397..483
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 397..413
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 434..473
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 804 AA; 89073 MW; F39B6A0A85DDD6DD CRC64;
MDDELLELQR QFEFAQQVKS SVRLSDRNVV ELVQKLQELG VIDFDLLHTV TGKEYITQEQ
LRNEITREIS KLGRVSVIDL ADTIGVDLYH VEKQAQDVVL NDPGLMLVQG EIISQSYWDS
IAEEINERLQ ECSQIAVAEL AGQLQVGSEL VQSVLEPRLG TLVKARLEGG QLYTPAYVER
VTAMVRGASR GIFVPSNLSA LWAPLQQLVQ ETNGASGVAV ENSFFQSIFN RLLKEEEMLG
SLRAGTHWTP SAFATAQKEC VDSSFSQNSY ISYESMQKLG ISQAVQFLQS RYPDGTPLAA
VFIHSSMIEM LDSATEDAIE QNSWIDSLSV LPSSFTSQDA NKMLLLCPSV QSALKAEKAL
ILGESYVLSS GFIKGIYDQI EKEADAFSIQ ASTATLIHPS SKSSESTESI PANTDKGSKK
KKGKSASTKA ATVETVPDDE EDARPKSKRN QKKGRDSSSS QKLDSKAGGK KESVKAQESN
NIIPPDEWVM KKIVDSVPEF EDDGTENPDS ILKHLADHMK PMLINSLKER RKKIFTENAD
RMRRLIDDLQ KKLDESFLNM QLYEKALDLF EDDQSTAVVL HRHLLRTTAA TIADTLLHGL
DIHNKMKNGT EVEESKTQDL VLLDSSERTA LAKNLNGSLS KKALALVEAL EGKRVDTFMV
TFRDLAEESG LVLKKLDKKL ERTLLHSYRK DLISQVSTES DPIALLAKVV SLLFIKIHNK
ALQAPGRAIA AAISHLKEKL DESAYKTLTD YQTATVTLLA LMSASSGEEH DCSADRILTK
RELLESQMPL LRTLVLGDSQ PQQS
//
