ID Q9RM86_CLOAM Unreviewed; 438 AA.
AC Q9RM86;
DT 01-MAY-2000, integrated into UniProtKB/TrEMBL.
DT 01-JUL-2008, sequence version 2.
DT 27-MAR-2024, entry version 80.
DE SubName: Full=4-Hydroxybutyrate CoA-transferase {ECO:0000313|EMBL:CAB60036.2};
DE SubName: Full=Acetyl-CoA hydrolase/transferase family protein {ECO:0000313|EMBL:MBN7772164.1};
GN Name=abfT {ECO:0000313|EMBL:CAB60036.2};
GN ORFNames=JYB65_02205 {ECO:0000313|EMBL:MBN7772164.1};
OS Clostridium aminobutyricum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=33953 {ECO:0000313|EMBL:CAB60036.2};
RN [1] {ECO:0000313|EMBL:CAB60036.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSMZ 2634 {ECO:0000313|EMBL:CAB60036.2};
RX PubMed=11041350; DOI=10.1007/s002030000195;
RA Gerhardt A., Linder D., Huisman G., Buckel W.;
RT "Fermentation of 4-aminobutyrate by Clostridium aminobutyricum: cloning of
RT two genes involved in the formation and dehydration of 4-hydroxybutyryl-
RT CoA.";
RL Arch. Microbiol. 174:189-199(2000).
RN [2] {ECO:0000313|EMBL:CAB60036.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSMZ 2634 {ECO:0000313|EMBL:CAB60036.2};
RA Lee T.-M., Wu T.-M., Sung M.-S.;
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0007829|PDB:3GK7}
RP X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH SPERMIDINE.
RX PubMed=19804364; DOI=10.1515/bc.2009.147;
RA Macieira S., Zhang J., Velarde M., Buckel W., Messerschmidt A.;
RT "Crystal structure of 4-hydroxybutyrate CoA-transferase from Clostridium
RT aminobutyricum.";
RL Biol. Chem. 390:1251-1263(2009).
RN [4] {ECO:0007829|PDB:3QDQ}
RP X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH COENZYME A.
RX PubMed=21833509; DOI=10.1007/s00203-011-0737-2;
RA Macieira S., Zhang J., Buckel W., Messerschmidt A.;
RT "Crystal structure of the complex between 4-hydroxybutyrate CoA-transferase
RT from Clostridium aminobutyricum and CoA.";
RL Arch. Microbiol. 194:157-166(2012).
RN [5] {ECO:0000313|EMBL:MBN7772164.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 2634 {ECO:0000313|EMBL:MBN7772164.1};
RA Kim Y.J., Yang J.-A.;
RT "Abyssanaerobacter marinus gen.nov., sp., nov, anaerobic bacterium isolated
RT from the Onnuri vent field of Indian Ocean and suggestion of
RT Mogibacteriaceae fam. nov., and proposal of reclassification of ambiguous
RT this family's genus member.";
RL Submitted (FEB-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the acetyl-CoA hydrolase/transferase family.
CC {ECO:0000256|ARBA:ARBA00009632}.
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DR EMBL; AJ250267; CAB60036.2; -; Genomic_DNA.
DR EMBL; JAFJZZ010000001; MBN7772164.1; -; Genomic_DNA.
DR PDB; 3GK7; X-ray; 1.85 A; A/B=1-438.
DR PDB; 3QDQ; X-ray; 2.60 A; A=1-438.
DR PDBsum; 3GK7; -.
DR PDBsum; 3QDQ; -.
DR KEGG; ag:CAB60036; -.
DR BioCyc; MetaCyc:MONOMER-13484; -.
DR Proteomes; UP000664545; Unassembled WGS sequence.
DR GO; GO:0008775; F:acetate CoA-transferase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006083; P:acetate metabolic process; IEA:InterPro.
DR Gene3D; 3.30.750.70; 4-hydroxybutyrate coenzyme like domains; 1.
DR Gene3D; 3.40.1080.20; Acetyl-CoA hydrolase/transferase C-terminal domain; 1.
DR Gene3D; 3.40.1080.10; Glutaconate Coenzyme A-transferase; 1.
DR InterPro; IPR026888; AcetylCoA_hyd_C.
DR InterPro; IPR038460; AcetylCoA_hyd_C_sf.
DR InterPro; IPR046433; ActCoA_hydro.
DR InterPro; IPR003702; ActCoA_hydro_N.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR PANTHER; PTHR21432:SF20; ACETYL-COA HYDROLASE; 1.
DR PANTHER; PTHR21432; ACETYL-COA HYDROLASE-RELATED; 1.
DR Pfam; PF13336; AcetylCoA_hyd_C; 1.
DR Pfam; PF02550; AcetylCoA_hydro; 1.
DR SUPFAM; SSF100950; NagB/RpiA/CoA transferase-like; 2.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:3GK7, ECO:0007829|PDB:3QDQ};
KW Hydrolase {ECO:0000313|EMBL:MBN7772164.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000664545};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:CAB60036.2}.
FT DOMAIN 7..178
FT /note="Acetyl-CoA hydrolase/transferase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02550"
FT DOMAIN 272..429
FT /note="Acetyl-CoA hydrolase/transferase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13336"
FT BINDING 216
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0007829|PDB:3QDQ"
FT BINDING 218
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0007829|PDB:3QDQ"
FT BINDING 238
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0007829|PDB:3QDQ"
FT BINDING 313
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0007829|PDB:3QDQ"
FT BINDING 332
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0007829|PDB:3QDQ"
FT BINDING 337
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0007829|PDB:3QDQ"
FT BINDING 372
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0007829|PDB:3QDQ"
SQ SEQUENCE 438 AA; 47697 MW; BFBD3241C20FFE73 CRC64;
MDWKKIYEDR TCTADEAVKS IKSGDRVLFA HCVAEPPVLV EAMVANAAAY KNVTVSHMVT
LGKGEYSKPE YKENFTFEGW FTSPSTRGSI AEGHGQFVPV FFHEVPSLIR KDIFHVDVFM
VMVSPPDHNG FCCVGVSSDY TMQAIKSAKI VLAEVNDQVP VVYGDTFVHV SEIDKFVETS
HPLPEIGLPK IGEVEAAIGK HCASLIEDGS TLQLGIGAIP DAVLSQLKDK KHLGIHSEMI
SDGVVDLYEA GVIDCSQKSI DKGKMAITFL MGTKRLYDFA ANNPKVELKP VDYINHPSVV
AQCSKMVCIN ACLQVDFMGQ IVSDSIGTKQ FSGVGGQVDF VRGASMSIDG KGKAIIAMPS
VAKKKDGSMI SKIVPFIDHG AAVTTSRNDA DYVVTEYGIA EMKGKSLQDR ARALINIAHP
DFKDELKAEF EKRFNAAF
//
