ID Q9V1X8_PYRAB Unreviewed; 623 AA.
AC Q9V1X8;
DT 01-MAY-2000, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2000, sequence version 1.
DT 27-MAR-2024, entry version 109.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=PAB2149 {ECO:0000313|EMBL:CAB49220.1};
OS Pyrococcus abyssi (strain GE5 / Orsay).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=272844 {ECO:0000313|EMBL:CAB49220.1, ECO:0000313|Proteomes:UP000000810};
RN [1] {ECO:0000313|EMBL:CAB49220.1, ECO:0000313|Proteomes:UP000000810}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GE5 / Orsay {ECO:0000313|Proteomes:UP000000810};
RX PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA Cohen G., Barbe V., Flament D., Galperin M., Heilig R., Ripp R.,
RA Lecompte O., Prieur D., Poch O., Quellerou J., Thierry J.C.,
RA Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.;
RT "An integrated analysis of the genome of the hyperthermophilic archaeon
RT Pyrococcus abyssi.";
RL Mol. Microbiol. 47:1495-1512(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ248283; CAB49220.1; -; Genomic_DNA.
DR PIR; E75221; E75221.
DR AlphaFoldDB; Q9V1X8; -.
DR STRING; 272844.PAB2149; -.
DR REBASE; 4171; M.PabORF2149P.
DR KEGG; pab:PAB2149; -.
DR PATRIC; fig|272844.11.peg.318; -.
DR eggNOG; arCOG02632; Archaea.
DR HOGENOM; CLU_013049_4_2_2; -.
DR PhylomeDB; Q9V1X8; -.
DR Proteomes; UP000000810; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR GO; GO:1901576; P:organic substance biosynthetic process; IEA:UniProt.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 124..251
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 262..584
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 623 AA; 72680 MW; B87C455390DD8A03 CRC64;
MVTPMPRSKT KTKNGVKNGV DEWKILEELQ PWVVKRYLLI KEAFKDKEFT QEDVEKLFEE
KRKELMEKGI KEPEFTTKNV GEILSILRKA GLIIARKDVY DFRRTYYRLK FPTETKISRD
RLISLLKAAA DQIRGGLDYK ALLVFLFYKA ISDRWMKMAQ DLMKEGKTKT QAYILTNKRY
YNLFDEDTGK LYTWHEVVKS RETIKEMANA LIKISEMNEE LADLKKLVEV LGLIGFIKED
NLHKLEEIVK IFNRVDFAEF DSDILGDAYE WILSYFAPQK AKEGEVYTPR EVIRLLVELL
DIEDGSDILD PASGSGGMLI EAYRYVKEKL KKEGFDEEPA IMLYGQELNE VTAALSKLNL
ILHGIQEFKI FEGADSLVNP QWEEELKRNG IEDGKVDYVI ANPPWNQDGY DETRLSDRRI
KHIYKYGYTS KQSADWAWVQ LMLYYARRKV GIVLDTGALF RGGAEKAIRQ GIVEDDLIEA
IILLPEKLFY NAAAPGIIMV LNPNKPEERK GKILFINASR EFRKHPEVRK LNQLADEHIR
KIVDAYREFK EIEGFSRVVT LEEIRKNDYN LNVSLYVFPE DEREQIDLAK EFKEFKEIEK
RERELVEKAK AYIEGILGAM DNE
//
