ID Q9WX87_CLOPF Unreviewed; 425 AA.
AC Q9WX87;
DT 01-NOV-1999, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1999, sequence version 1.
DT 24-JAN-2024, entry version 74.
DE SubName: Full=FAD-dependent oxidoreductase {ECO:0000313|EMBL:NGU29564.1, ECO:0000313|EMBL:SQB57770.1};
DE SubName: Full=Hypothetical 46.7-kDa protein {ECO:0000313|EMBL:BAA74789.1};
GN ORFNames=G6Z34_05465 {ECO:0000313|EMBL:NGU29564.1}, NCTC10719_00362
GN {ECO:0000313|EMBL:SQB57770.1};
OS Clostridium perfringens.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1502 {ECO:0000313|EMBL:BAA74789.1};
RN [1] {ECO:0000313|EMBL:BAA74789.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=PB6K {ECO:0000313|EMBL:BAA74789.1};
RX PubMed=10627036;
RA Fujinaga K., Taniguchi Y., Sun Y., Katayama S., Minami J., Matsushita O.,
RA Okabe A.;
RT "Analysis of genes involved in nitrate reduction in Clostridium
RT perfringens.";
RL Microbiology 145:3377-3387(1999).
RN [2] {ECO:0000313|EMBL:SQB57770.1, ECO:0000313|Proteomes:UP000249986}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC10719 {ECO:0000313|EMBL:SQB57770.1,
RC ECO:0000313|Proteomes:UP000249986};
RG Pathogen Informatics;
RA Doyle S.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:NGU29564.1, ECO:0000313|Proteomes:UP000481454}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CP-40 {ECO:0000313|EMBL:NGU29564.1,
RC ECO:0000313|Proteomes:UP000481454};
RA Feng Y., Hu Y.;
RT "Genomic Insights into the Phylogeny and Genetic Plasticity of the Human
RT and Animal Enteric Pathogen Clostridium perfringens.";
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: NAD-binding protein involved in the addition of a
CC carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC certain tRNAs, forming tRNA-cmnm(5)s(2)U34.
CC {ECO:0000256|ARBA:ARBA00003717}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC {ECO:0000256|ARBA:ARBA00025948}.
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DR EMBL; AB017192; BAA74789.1; -; Genomic_DNA.
DR EMBL; JAALLZ010000001; NGU29564.1; -; Genomic_DNA.
DR EMBL; UAWG01000001; SQB57770.1; -; Genomic_DNA.
DR RefSeq; WP_003451172.1; NZ_UFWR01000001.1.
DR AlphaFoldDB; Q9WX87; -.
DR PATRIC; fig|1502.176.peg.2160; -.
DR Proteomes; UP000249986; Unassembled WGS sequence.
DR Proteomes; UP000481454; Unassembled WGS sequence.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR002218; MnmG-rel.
DR InterPro; IPR040131; MnmG_N.
DR PANTHER; PTHR11806; GLUCOSE INHIBITED DIVISION PROTEIN A; 1.
DR PANTHER; PTHR11806:SF0; PROTEIN MTO1 HOMOLOG, MITOCHONDRIAL; 1.
DR Pfam; PF01134; GIDA; 2.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE 4: Predicted;
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 2..226
FT /note="MnmG N-terminal"
FT /evidence="ECO:0000259|Pfam:PF01134"
FT DOMAIN 261..350
FT /note="MnmG N-terminal"
FT /evidence="ECO:0000259|Pfam:PF01134"
SQ SEQUENCE 425 AA; 46679 MW; C8603702B7D853CB CRC64;
MKVVVIGGGW SGVAAAIEAK KMGADVVLFE KTDLLLGLGN VGGIMRNNGR YTASEELIAM
GGGDLINITD KVSRHKDIPF PGHEHAWLYD VNLVEGEVKR YVESLGIETK MVSRIIDIKQ
EDNKILGVYT SDGQYYKGDV FIETTGSTGP MGNCLRYGNG CSMCILRCPS FGPRISISSR
CGVEDIQGER VGDELGAFSG SCKLAKETLS EEIRKELEEK GCVVLKIPKE DVNYDKLNTK
VCQQYALKEF AENVVLLDTG HAKLMTTYYP LEKLRKIKGL ENAKYVDPYA GGKGNSIRYL
SVAPRDDNMK VKGVTNLFCA GEKSGLFVGH TEAIVTGTLA GHNAVRHALG IPYLILPRAT
VLGDIIAFAN EESQSREGKK NRYTFAGSVY FNRMKELGLY TIDKDEIQKR VAQLNLDGVF
SKKLM
//
