ID TRUB_AERPE Reviewed; 354 AA.
AC Q9YDC3;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 27-MAR-2024, entry version 100.
DE RecName: Full=Probable tRNA pseudouridine synthase B;
DE EC=5.4.99.25;
DE AltName: Full=tRNA pseudouridine(55) synthase;
DE Short=Psi55 synthase;
DE AltName: Full=tRNA pseudouridylate synthase;
DE AltName: Full=tRNA-uridine isomerase;
GN Name=truB; OrderedLocusNames=APE_0989.1;
OS Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 /
OS K1).
OC Archaea; Thermoproteota; Thermoprotei; Desulfurococcales;
OC Desulfurococcaceae; Aeropyrum.
OX NCBI_TaxID=272557;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RX PubMed=10382966; DOI=10.1093/dnares/6.2.83;
RA Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K.,
RA Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A.,
RA Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S.,
RA Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H.;
RT "Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon,
RT Aeropyrum pernix K1.";
RL DNA Res. 6:83-101(1999).
RN [2]
RP SEQUENCE REVISION.
RA Tajima T., Sekigawa T., Oguchi A., Yamazaki S., Tanikawa S., Fujita N.,
RA Harayama S.;
RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC subfamily. {ECO:0000305}.
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DR EMBL; BA000002; BAA79974.2; -; Genomic_DNA.
DR PIR; F72696; F72696.
DR AlphaFoldDB; Q9YDC3; -.
DR SMR; Q9YDC3; -.
DR STRING; 272557.APE_0989.1; -.
DR EnsemblBacteria; BAA79974; BAA79974; APE_0989.1.
DR KEGG; ape:APE_0989.1; -.
DR eggNOG; arCOG00987; Archaea.
DR Proteomes; UP000002518; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR CDD; cd21148; PUA_Cbf5; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 2.30.130.10; PUA domain; 1.
DR HAMAP; MF_01081; TruB_arch; 1.
DR InterPro; IPR012960; Dyskerin-like.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR InterPro; IPR026326; TruB_arch.
DR InterPro; IPR032819; TruB_C.
DR NCBIfam; TIGR00425; CBF5; 1.
DR PANTHER; PTHR23127; CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5; 1.
DR PANTHER; PTHR23127:SF0; H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1; 1.
DR Pfam; PF08068; DKCLD; 1.
DR Pfam; PF01472; PUA; 1.
DR Pfam; PF16198; TruB_C_2; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SMART; SM01136; DKCLD; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR SUPFAM; SSF88697; PUA domain-like; 1.
DR PROSITE; PS50890; PUA; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..354
FT /note="Probable tRNA pseudouridine synthase B"
FT /id="PRO_0000121954"
FT DOMAIN 261..336
FT /note="PUA"
FT ACT_SITE 92
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 354 AA; 39732 MW; 405BC77A52E8DE43 CRC64;
MAEEAVDARG EGARFIEKVK SICGNTSRIL YKYDEPTDPR YGYLPHERPL DVYLRYGMIV
VDKPPGPTSH EVVAWIKRML GVSRAGHGGT LDPKVTGVLP VALERMTRII GTVMHSSKEY
VCVMQLHRPV EEDRLREVLK LFEGEIYQKP PLRSSVKRAL RTRRVFRIEL LEYTGKYALL
RVDCEAGTYM RKLCWDIGLV LGVGAHMREL RRIRTGPFSE DSGLMVRLDD VAYAVIRWRE
EGKDDLLRRV VIPGEYSVCH IPKVLVRDSA VESLTHGAQL AAPGVAAVEE GVEKGGMVAL
MTLKGELIGL GKALASAQEM LEAERGIVVS PTRIIMERGL YPRMWKRQQA PQGA
//
