ID Q9Z3X5_9RALS Unreviewed; 199 AA.
AC Q9Z3X5;
DT 01-MAY-1999, integrated into UniProtKB/TrEMBL.
DT 01-MAY-1999, sequence version 1.
DT 03-MAY-2023, entry version 71.
DE RecName: Full=2-hydroxychromene-2-carboxylate isomerase {ECO:0000256|PIRNR:PIRNR006386};
DE EC=5.99.1.4 {ECO:0000256|PIRNR:PIRNR006386};
GN Name=nagD {ECO:0000313|EMBL:AAD12617.1};
OS Ralstonia sp. U2.
OG Plasmid pWWU2 {ECO:0000313|EMBL:AAD12617.1}.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=70356 {ECO:0000313|EMBL:AAD12617.1};
RN [1] {ECO:0000313|EMBL:AAD12617.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=U2 {ECO:0000313|EMBL:AAD12617.1};
RC PLASMID=pWWU2 {ECO:0000313|EMBL:AAD12617.1};
RX PubMed=9573207;
RA Fuenmayor S.L., Wild M., Boyes A.L., Williams P.A.;
RT "A gene cluster encoding steps in conversion of naphthalene to gentisate in
RT Pseudomonas sp. strain U2.";
RL J. Bacteriol. 180:2522-2530(1998).
RN [2] {ECO:0000313|EMBL:AAD12617.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=U2 {ECO:0000313|EMBL:AAD12617.1};
RC PLASMID=pWWU2 {ECO:0000313|EMBL:AAD12617.1};
RA Fuenmayor S., Wild M., Boyes A.L., Williams P.A.;
RL Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:AAD12617.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=U2 {ECO:0000313|EMBL:AAD12617.1};
RC PLASMID=pWWU2 {ECO:0000313|EMBL:AAD12617.1};
RX PubMed=11133965; DOI=10.1128/JB.183.2.700-708.2001;
RA Zhou N.Y., Fuenmayor S.L., Williams P.A.;
RT "nag genes of Ralstonia (formerly Pseudomonas) sp. strain U2 encoding
RT enzymes for gentisate catabolism.";
RL J. Bacteriol. 183:700-708(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-hydroxychromene-2-carboxylate = (3E)-4-(2-hydroxyphenyl)-2-
CC oxobut-3-enoate; Xref=Rhea:RHEA:27401, ChEBI:CHEBI:59350,
CC ChEBI:CHEBI:59353; EC=5.99.1.4;
CC Evidence={ECO:0000256|PIRNR:PIRNR006386};
CC -!- SIMILARITY: Belongs to the GST superfamily. NadH family.
CC {ECO:0000256|PIRNR:PIRNR006386}.
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DR EMBL; AF036940; AAD12617.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9Z3X5; -.
DR GO; GO:0018845; F:2-hydroxychromene-2-carboxylate isomerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:1901170; P:naphthalene catabolic process; IEA:InterPro.
DR CDD; cd03022; DsbA_HCCA_Iso; 1.
DR Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR InterPro; IPR001853; DSBA-like_thioredoxin_dom.
DR InterPro; IPR014440; HCCAis_GSTk.
DR InterPro; IPR044087; NahD-like.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR42943; GLUTATHIONE S-TRANSFERASE KAPPA; 1.
DR PANTHER; PTHR42943:SF2; GLUTATHIONE S-TRANSFERASE KAPPA 1; 1.
DR Pfam; PF01323; DSBA; 1.
DR PIRSF; PIRSF006386; HCCAis_GSTk; 1.
DR SUPFAM; SSF52833; Thioredoxin-like; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|PIRNR:PIRNR006386, ECO:0000313|EMBL:AAD12617.1};
KW Plasmid {ECO:0000313|EMBL:AAD12617.1}.
FT DOMAIN 5..194
FT /note="DSBA-like thioredoxin"
FT /evidence="ECO:0000259|Pfam:PF01323"
FT ACT_SITE 13
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006386-1"
SQ SEQUENCE 199 AA; 23067 MW; 219466E06AC04E47 CRC64;
MIVMVDFYFD FLSPFSYLAN HRLSVLAGRY GFSIQYHAID LARAKTAIGN IGPSNRDLKV
KLDYLKVDLQ RWADLYRIPL VFPPNFNSRR VNAGLYYPAA RERAAEYVRL VFDSAWGKGW
ALDADSLLAE VCDKLNWDLG EFEDFLNSEN AAKAYDEETQ AAIDRKVFGV PTVFWDDQMW
WGNDRLFMLE SRLQKETQP
//
