ID R0CJP0_RALPI Unreviewed; 492 AA.
AC R0CJP0;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=Microcystinase C {ECO:0000256|PIRNR:PIRNR012702};
DE Short=MlrC {ECO:0000256|PIRNR:PIRNR012702};
GN ORFNames=OR214_03429 {ECO:0000313|EMBL:ENZ76690.1};
OS Ralstonia pickettii OR214.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=1264675 {ECO:0000313|EMBL:ENZ76690.1, ECO:0000313|Proteomes:UP000013280};
RN [1] {ECO:0000313|EMBL:ENZ76690.1, ECO:0000313|Proteomes:UP000013280}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OR214 {ECO:0000313|EMBL:ENZ76690.1,
RC ECO:0000313|Proteomes:UP000013280};
RX PubMed=23792748;
RA Utturkar S.M., Bollmann A., Brzoska R.M., Klingeman D.M., Epstein S.E.,
RA Palumbo A.V., Brown S.D.;
RT "Draft Genome Sequence for Ralstonia sp. Strain OR214, a Bacterium with
RT Potential for Bioremediation.";
RL Genome Announc. 1:0-0(2013).
CC -!- FUNCTION: Involved in peptidolytic degradation of cyclic heptapeptide
CC hepatotoxin microcystin (MC). {ECO:0000256|PIRNR:PIRNR012702}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRNR:PIRNR012702};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRNR:PIRNR012702};
CC -!- SIMILARITY: Belongs to the peptidase M81 family.
CC {ECO:0000256|PIRNR:PIRNR012702}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ENZ76690.1}.
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DR EMBL; APMQ01000009; ENZ76690.1; -; Genomic_DNA.
DR RefSeq; WP_004632561.1; NZ_APMQ01000009.1.
DR AlphaFoldDB; R0CJP0; -.
DR PATRIC; fig|1264675.3.peg.3360; -.
DR Proteomes; UP000013280; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR009197; MlrC.
DR InterPro; IPR010799; MlrC_C.
DR InterPro; IPR015995; MlrC_N.
DR Pfam; PF07364; DUF1485; 1.
DR Pfam; PF07171; MlrC_C; 1.
DR PIRSF; PIRSF012702; UCP012702; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|PIRNR:PIRNR012702};
KW Metal-binding {ECO:0000256|PIRNR:PIRNR012702};
KW Metalloprotease {ECO:0000256|PIRNR:PIRNR012702};
KW Protease {ECO:0000256|PIRNR:PIRNR012702}.
FT DOMAIN 2..282
FT /note="Microcystin LR degradation protein MlrC N-terminal"
FT /evidence="ECO:0000259|Pfam:PF07364"
FT DOMAIN 296..475
FT /note="Microcystin LR degradation protein MlrC C-terminal"
FT /evidence="ECO:0000259|Pfam:PF07171"
SQ SEQUENCE 492 AA; 51675 MW; 2FF72277A96DDE2F CRC64;
MRILIARFNH ETNTFSPVPT PLASFHPHYG ADAYRAAKGT RTAAGAFIDL AEAAGAEIVV
PVVAGANPSG RVAAEAYTTL CDAIVAAAPG CDALMLDLHG AMVAENSDDG EGDLLERLRA
VAPGVPIAVA LDLHGNLTQK FIDLADIAVS FKTYPHVDMY ETGEHAGRLL FDKLAGRANP
VLAWRRLPMV THTLRSRTDE GAMHRAVALA KQAETDGMLA VSILAGFGLA DIAAPCLSVV
VVGNGDKHAA DAAAQRIADA IWADREGFVY RSEPLTESIA RAAKLAHAPG SGPVLLLDHG
DNCMSGGTCD DMHVLHEALK QGLSGIAVGP VCDPEAVAAM IEAGEGATVS LPVGDKVPLV
QLGIYPVPRA LTGKVVGISD GEYTITGPTY TGQRIRMGRT ALLDIGAAQI VVTETPQEHW
DLGIFTHIGV DPHAARFVLL KSRMYCRPVF VPIAKAVVEC DGGGVTSSDY SRFPFVKVQR
PVFPLDADAV PA
//
