ID R0GKG5_9BRAS Unreviewed; 1531 AA.
AC R0GKG5;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 50.
DE RecName: Full=DNA (cytosine-5)-methyltransferase {ECO:0000256|PIRNR:PIRNR037404};
DE EC=2.1.1.37 {ECO:0000256|PIRNR:PIRNR037404};
GN ORFNames=CARUB_v10025739mg {ECO:0000313|EMBL:EOA12790.1};
OS Capsella rubella.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Capsella.
OX NCBI_TaxID=81985 {ECO:0000313|EMBL:EOA12790.1, ECO:0000313|Proteomes:UP000029121};
RN [1] {ECO:0000313|Proteomes:UP000029121}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Monte Gargano {ECO:0000313|Proteomes:UP000029121};
RX PubMed=23749190; DOI=10.1038/ng.2669;
RA Slotte T., Hazzouri K.M., Agren J.A., Koenig D., Maumus F., Guo Y.L.,
RA Steige K., Platts A.E., Escobar J.S., Newman L.K., Wang W., Mandakova T.,
RA Vello E., Smith L.M., Henz S.R., Steffen J., Takuno S., Brandvain Y.,
RA Coop G., Andolfatto P., Hu T.T., Blanchette M., Clark R.M., Quesneville H.,
RA Nordborg M., Gaut B.S., Lysak M.A., Jenkins J., Grimwood J., Chapman J.,
RA Prochnik S., Shu S., Rokhsar D., Schmutz J., Weigel D., Wright S.I.;
RT "The Capsella rubella genome and the genomic consequences of rapid mating
RT system evolution.";
RL Nat. Genet. 45:831-835(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-
CC methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:13681, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:11370,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:85452, ChEBI:CHEBI:85454; EC=2.1.1.37;
CC Evidence={ECO:0000256|PIRNR:PIRNR037404,
CC ECO:0000256|RuleBase:RU000417};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|PIRNR:PIRNR037404}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. C5-methyltransferase family.
CC {ECO:0000256|PIRNR:PIRNR037404, ECO:0000256|PROSITE-ProRule:PRU01016,
CC ECO:0000256|RuleBase:RU000416}.
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DR EMBL; KB870812; EOA12790.1; -; Genomic_DNA.
DR RefSeq; XP_006279892.1; XM_006279830.1.
DR STRING; 81985.R0GKG5; -.
DR GeneID; 17876824; -.
DR KEGG; crb:17876824; -.
DR eggNOG; ENOG502QPKK; Eukaryota.
DR OrthoDB; 317994at2759; -.
DR Proteomes; UP000029121; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003682; F:chromatin binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003886; F:DNA (cytosine-5-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR CDD; cd04712; BAH_DCM_I; 1.
DR CDD; cd04708; BAH_plantDCM_II; 1.
DR Gene3D; 2.30.30.490; -; 2.
DR Gene3D; 3.90.120.10; DNA Methylase, subunit A, domain 2; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR001025; BAH_dom.
DR InterPro; IPR043151; BAH_sf.
DR InterPro; IPR018117; C5_DNA_meth_AS.
DR InterPro; IPR001525; C5_MeTfrase.
DR InterPro; IPR031303; C5_meth_CS.
DR InterPro; IPR022702; Cytosine_MeTrfase1_RFD.
DR InterPro; IPR017198; DNMT1-like.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR00675; dcm; 1.
DR PANTHER; PTHR10629; CYTOSINE-SPECIFIC METHYLTRANSFERASE; 1.
DR PANTHER; PTHR10629:SF52; DNA (CYTOSINE-5)-METHYLTRANSFERASE 1; 1.
DR Pfam; PF01426; BAH; 2.
DR Pfam; PF00145; DNA_methylase; 2.
DR Pfam; PF12047; DNMT1-RFD; 2.
DR PIRSF; PIRSF037404; DNMT1; 5.
DR PRINTS; PR00105; C5METTRFRASE.
DR SMART; SM00439; BAH; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51038; BAH; 2.
DR PROSITE; PS00094; C5_MTASE_1; 1.
DR PROSITE; PS00095; C5_MTASE_2; 1.
DR PROSITE; PS51679; SAM_MT_C5; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PIRNR:PIRNR037404};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000256|PIRNR:PIRNR037404};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR037404};
KW Reference proteome {ECO:0000313|Proteomes:UP000029121};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PIRNR:PIRNR037404};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR037404}.
FT DOMAIN 736..870
FT /note="BAH"
FT /evidence="ECO:0000259|PROSITE:PS51038"
FT DOMAIN 910..1050
FT /note="BAH"
FT /evidence="ECO:0000259|PROSITE:PS51038"
FT REGION 657..708
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 657..687
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 688..708
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1195
FT /evidence="ECO:0000256|PIRSR:PIRSR037404-1,
FT ECO:0000256|PROSITE-ProRule:PRU01016"
SQ SEQUENCE 1531 AA; 172295 MW; 2863043147BD50A5 CRC64;
MVENGVKAMN GKKRALPEIK ETEDVPRTRR PRRAAACTDF KEKSVRLCEK SATIELKKQQ
TVDEEIVAIM LTAKEGGDAE PRPTRRLNDF VLFDSEGDLQ PVEMLEISDI FVSGAILPSD
VCSDKEKEKG VRCPSFGRVE NWSISGYEDG SPVIWISTEL ADYDCCKPAA RYKKVYDYFY
QKACASVAVY KKLSKSSGGD PDICLDELLA AVARSMSGSK YFSSGGAIQD FVISQGEFIY
NQLAGLDETA KKHETSYAEI PVLVALRDES SKIDKAAQIQ GRPSNGVLRI DGVSEVAVTN
ALTSKQLVDG ADDDRRYAKL LQDEEYRKSM QRPRKNSSSA SASNKFYIKI NENEIASDYP
LPSYYKNTKE ETDELMLSDA LYELDAEDLP QRMLHNWTLY NSDSRMISLE LLPMKHCDDT
DVSIFGSGMV TDDNLCWFSP DDPDSGSHSE DPDGMSIFLS QIKEWKIEYG GEYISISIRT
DMAWYRLGKP SKQYAPWYKP VLKTATVGIS ILTLLNEQSR LARFSFADVT KRLSGLQTND
KAYISSDLLS VQRFLIVHGQ IIIQLFAAYP NKKFIKCPFV VGLSSKLEDR HHTKWIIKNK
KILQKQLNLN PRAEMAPVVS KRKAMRATTT RLVHRIWGEF YSNYSPEDSL QAIGAANEEE
EVEDQGENGE EEVEEEGENE DVTEETVPEP VEVQKPHTPK KIRASSGKRE IRWDGESLGK
TSAGEPLYRQ ALVGGETVVV GGAVALEIDV SDELLAIYFV EYMFESTDHC KMLHGRFLQR
GSATVLGNAS NERELFLTNE CMTVQLKDIK GVVGFEIRSR PWGHQYRKEN IAADKLDRER
ALERKAKDLP TEYYCKSLYS PERGGFFSLP LSDIGRSSGF CYSCKIREDE EERSTTKLNV
SNTGFFSNGI EYSIEDFVYV NPDSIDGLKE GSKTVFKSGR NIGLRAYVVC QLLEIVPKES
RKADLGSFDV KVRRFYRPED VSAEKAYASD IQELYYSQDT DVLHPGALEG KCEVRKKNDM
PLCREYPISD HIFFCELFFD SSKGSLKQMP ANMKVKFSTI KDDTLLRKKK GKGVEGETES
GIVKPKEMRL ATLDIFAGCG GLSQGLEKAG VSDTKWAIEY EEPAGQAFRQ NHPASTVFVD
NCNVILRAIM EKGGDQKDCV STTEADELAA KLSEDQKKTL PLPGQVDFIN GGPPCQGFSG
MNRFNQSSWS KVQCEMILAF LSFADYFRPR YFLLENVRTF VSFNKGQTFQ LTLASLLEMG
YQVRFGILEA GAYGVSQSRK RAFIWAAAPE EVLPEWPEPM HVFGVPKLKI SLSQGLHYAA
VRSTAPGAPF RPITVRDTIG DLPAVENGES RINKEYKAEP VSWFQKEIRG DMISLTDHIC
KEMNEINLIR CKIIPKRPGA DWRDLPDEKV KLSTGQVVEM IPWCLPNTAK RHNQWKGLYG
RLDWQGNFPT SITDPQPMGK VGMCFHPDQD RILTVRECAR SQGFPDSYQF FGNIIHKHRQ
IGNAVPPPLA FALGRKLKEA LQLKNSLQHQ P
//
