ID R0KSI0_NOSB1 Unreviewed; 339 AA.
AC R0KSI0;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE SubName: Full=Putative methyltransferase NSUN5 {ECO:0000313|EMBL:EOB13726.1};
GN Name=NSUN5 {ECO:0000313|EMBL:EOB13726.1};
GN ORFNames=NBO_62g0016 {ECO:0000313|EMBL:EOB13726.1};
OS Nosema bombycis (strain CQ1 / CVCC 102059) (Microsporidian parasite)
OS (Pebrine of silkworm).
OC Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Nosematidae; Nosema.
OX NCBI_TaxID=578461 {ECO:0000313|EMBL:EOB13726.1, ECO:0000313|Proteomes:UP000016927};
RN [1] {ECO:0000313|EMBL:EOB13726.1, ECO:0000313|Proteomes:UP000016927}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CQ1 / CVCC 102059 {ECO:0000313|Proteomes:UP000016927};
RX PubMed=23496955; DOI=10.1186/1471-2164-14-186;
RA Pan G., Xu J., Li T., Xia Q., Liu S.L., Zhang G., Li S., Li C., Liu H.,
RA Yang L., Liu T., Zhang X., Wu Z., Fan W., Dang X., Xiang H., Tao M., Li Y.,
RA Hu J., Li Z., Lin L., Luo J., Geng L., Wang L., Long M., Wan Y., He N.,
RA Zhang Z., Lu C., Keeling P.J., Wang J., Xiang Z., Zhou Z.;
RT "Comparative genomics of parasitic silkworm microsporidia reveal an
RT association between genome expansion and host adaptation.";
RL BMC Genomics 14:186-186(2013).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
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DR EMBL; KB908970; EOB13726.1; -; Genomic_DNA.
DR AlphaFoldDB; R0KSI0; -.
DR STRING; 578461.R0KSI0; -.
DR EnsemblFungi; EOB13726; EOB13726; NBO_62g0016.
DR VEuPathDB; MicrosporidiaDB:NBO_62g0016; -.
DR HOGENOM; CLU_005316_7_4_1; -.
DR OMA; IRSKWHA; -.
DR Proteomes; UP000016927; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IEA:UniProt.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22807:SF4; 28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000016927};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 77..339
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT ACT_SITE 297
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 182..188
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 206
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 233
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 251
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 339 AA; 39622 MW; 0DC0CB9D66EFF227 CRC64;
MKIPKKYREF YTECSLMLKK IIEKKSTIKR EVYKKKNPSE YLAFLDKLVQ NYDLLDKIIT
AHEFLRKDKF YSVILCYEIL NDKINSQFWK TKFEETKGES EIQHVVKQKF IRLNTLKAKE
EDLKEMKCDL TSIPNVYKLC SNIDFSTSQL FKDGKIIVQN IASCLPAFLL NPEKDSRVID
ACSAPGNKTS HLSAIMNNTG KVFAVEVDKY RYNLMKGRLN RFGVKNVKYL NKDFFDIDKD
EFKNIDYILV DPTCSGSGTH DYYNEDKERT EGLHKFQTKL LKHAMKFGAK KIVYSTCSNN
KEEGEDVVKA AIAESEYEIY DSSKEYKFLE DLFKFSDLL
//
