ID R4G792_9BACI Unreviewed; 724 AA.
AC R4G792;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364053};
DE EC=5.6.2.4 {ECO:0000256|RuleBase:RU364053};
GN ORFNames=KN10_2713 {ECO:0000313|EMBL:GAC92277.1};
OS Anoxybacillus flavithermus NBRC 109594.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Anoxybacillus.
OX NCBI_TaxID=1315967 {ECO:0000313|EMBL:GAC92277.1, ECO:0000313|Proteomes:UP000013057};
RN [1] {ECO:0000313|Proteomes:UP000013057}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 109594 {ECO:0000313|Proteomes:UP000013057};
RA Matsutani M., Shirakihara Y., Imada K., Yakushi T., Matsushita K.;
RT "Draft Genome Sequence of a Thermophilic Member of the Bacillaceae,
RT Anoxybacillus flavithermus Strain Kn10, Isolated from the Kan-nawa Hot
RT Spring in Japan.";
RL Genome Announc.1:e00311-13(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618,
CC ECO:0000256|RuleBase:RU364053};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922, ECO:0000256|RuleBase:RU364053}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAC92277.1}.
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DR EMBL; BARH01000032; GAC92277.1; -; Genomic_DNA.
DR AlphaFoldDB; R4G792; -.
DR Proteomes; UP000013057; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR01073; pcrA; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA-binding {ECO:0000256|RuleBase:RU364053};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 13..292
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 293..572
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 34..41
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 724 AA; 82864 MW; 9799E61EAF561AAB CRC64;
MEKMSMSAHM LLEGLNEKQK EAVKTTEGPL LIMAGAGSGK TRVLTHRIAY LMAEKDVAPW
NILAITFTNK AAREMKERVE RIVGKQAEDI WISTFHSMCV RILRRDIDRI GIHANFSILD
PTDQLSVLKH ILKDRNIDPK KYDPRALLGT ISSAKNELMT PEKYADQAAN PYEKLVAEVY
KEYQKRLLRN HALDFDDLIM TTIQLFQRVP EVLEYYQRKF QYIHVDEYQD TNRSQYLLVN
MLAKRFQNLC VVGDSDQSIY RWRGADISNI LSFEKDYPNA KVILLEQNYR STKRILQAAN
AVIEHNTQRK PKKLWTENSE GHALVYYEAM TEVDEAQFVV GKIKEYVDAG KRTYKDFAVL
YRTNAQSRVL EEMLLKANIP YKIVGGLKFY DRKEIKDILA YLRLIANPND DISLTRIINV
PKRGIGASTI DKIAAYASSQ GISMFDALGS IEEMGISARI AAPLVQFREQ IHHWSQMQHY
LSVTELVEDV LDRSGYRDML REEKTLEAHS RLENIDEFLS VTQHFEQVNE DKSLVAFLTD
LALVADIDQL DNNSEGQTND AVVLMTLHSA KGLEFPVVFL VGLEEGIFPH NRSLNDEDEM
EEERRLAYVG ITRAEEELFL TRAHMRTLFG QTHVNAPSRF IDEIPEELIE YAHKRVKKSS
ASVRPSTASQ GEWRVGDKVS HKVWGVGTIV RVKESDGDQE LDVAFSSPIG IKRLLAKFAP
ITKV
//
