ID   R5BVZ2_9FIRM            Unreviewed;       648 AA.
AC   R5BVZ2;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 32.
DE   SubName: Full=Radical SAM domain-containing protein {ECO:0000313|EMBL:CCX59795.1};
GN   ORFNames=BN499_03021 {ECO:0000313|EMBL:CCX59795.1};
OS   Blautia hydrogenotrophica CAG:147.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae; Blautia.
OX   NCBI_TaxID=1263061 {ECO:0000313|EMBL:CCX59795.1, ECO:0000313|Proteomes:UP000018163};
RN   [1] {ECO:0000313|EMBL:CCX59795.1, ECO:0000313|Proteomes:UP000018163}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:147 {ECO:0000313|Proteomes:UP000018163};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CCX59795.1}.
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DR   EMBL; CAWR010000211; CCX59795.1; -; Genomic_DNA.
DR   AlphaFoldDB; R5BVZ2; -.
DR   Proteomes; UP000018163; Unassembled WGS sequence.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd03801; GT4_PimA-like; 1.
DR   CDD; cd01335; Radical_SAM; 1.
DR   CDD; cd21109; SPASM; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR   InterPro; IPR023885; 4Fe4S-binding_SPASM_dom.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR007197; rSAM.
DR   PANTHER; PTHR11228:SF7; PQQA PEPTIDE CYCLASE; 1.
DR   PANTHER; PTHR11228; RADICAL SAM DOMAIN PROTEIN; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF13186; SPASM; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE   4: Predicted;
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018163};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT   DOMAIN          59..208
FT                   /note="Glycosyl transferase family 1"
FT                   /evidence="ECO:0000259|Pfam:PF00534"
FT   DOMAIN          264..401
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|Pfam:PF04055"
FT   DOMAIN          488..547
FT                   /note="4Fe4S-binding SPASM"
FT                   /evidence="ECO:0000259|Pfam:PF13186"
SQ   SEQUENCE   648 AA;  74340 MW;  226F25C5DBC9A299 CRC64;
     MAKSAIGSLE ATFWKLNGAY KYIDAMICCS EFMKTKMDSN PMFANKTVAV HNFVEQMEWK
     ETEKKDYVLY FGRFSKEKGI ETLLKVCREL SDVSFIFAGT GPLEKEINSI SNIKNVGFQT
     GEALETLIRE ARFSIYPSEW YENCPFSVME SQMYGTPVLG ANIGGIPELI EVGKTGELFE
     SGNASELKKN IQKLWADQAL TDQYSRNCKD ISFDDIEAYT KKLMKIYRGK KKTTGSVKAT
     DAVVKGDKNM GKKSNSEKKL NGTVIVTYRC NARCSMCNRY KAPSKPEEEI SIETIRKLPK
     MYFTNITGGE PFIRTDLKEI VRELYKKSDR IVISTNGFFT ERIVELCKEF PQIGIRISIE
     GLEQTNNEIR GLQDGYQRGY KTLKTLRKMG MKDVGFGMTV QDKNAPDLVP LYKISDKMDM
     EFATASLHNS FYFVEAKNII HDRPMVAKNF ENLVNELLRS NSPKKWFRAY FNHGLINYIY
     GQKRLLPCDM SFDTFFIDPY GDVMPCNGTK DKEVMGNLNT QTWDELWNSP EAEKVRKKVR
     CCDRNCWMIG SVSPAMHKYI WKPALWVVKH KFKALFSKHP YRMYELKVCR DYRDGKVTKE
     QLDKCSTCDF HAVINDGLSA ASKEQLKGRS GEEIVDADIA RQLRGKNE
//