ID   R5L0N6_9FIRM            Unreviewed;       489 AA.
AC   R5L0N6;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   24-JAN-2024, entry version 24.
DE   SubName: Full=Glycosyl hydrolase family 43 {ECO:0000313|EMBL:CCY72648.1};
GN   ORFNames=BN470_00994 {ECO:0000313|EMBL:CCY72648.1};
OS   Eubacterium sp. CAG:115.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC   Eubacterium.
OX   NCBI_TaxID=1262878 {ECO:0000313|EMBL:CCY72648.1, ECO:0000313|Proteomes:UP000018005};
RN   [1] {ECO:0000313|EMBL:CCY72648.1, ECO:0000313|Proteomes:UP000018005}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:115 {ECO:0000313|Proteomes:UP000018005};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CCY72648.1}.
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DR   EMBL; CAYS010000060; CCY72648.1; -; Genomic_DNA.
DR   AlphaFoldDB; R5L0N6; -.
DR   STRING; 1262878.BN470_00994; -.
DR   Proteomes; UP000018005; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd18617; GH43_XynB-like; 1.
DR   Gene3D; 2.60.120.200; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR041542; GH43_C2.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR   PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR   Pfam; PF17851; GH43_C2; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR   SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018005}.
FT   DOMAIN          310..477
FT                   /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT                   /evidence="ECO:0000259|Pfam:PF17851"
FT   ACT_SITE        14
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        174
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            120
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   489 AA;  54879 MW;  7BA6DC13B8B75887 CRC64;
     MIYHNPVLKG FYPDPSVCSY NGKYYMVCSS FQYFPGVPLF ESSDLVNWKQ IGYVLTRPSQ
     VMLDKINSSG GVFAPTIRCS NGRFYMVTTN DTTHQNFYVY TDDIYGEWSE PIYVDQGGID
     PSLFFEDGRT YFMSNGADDN GVGGVVQCEI DIATGKKLST SCCIWQGSGG RYLESPHLYK
     INGVYYLMAA EGGTEYGHMI TCARADSVWG KFEGNPKNPV LTNRNKAPFI IQGIGHGDLV
     QDEYGDWHVL SLGFRQQHIW RPYHHLGREV FLTPVKFGED GWFTCGNDGT TDESYEIKGD
     FTQNEQRVFT FANTFWDKEW CFMRRPVMEN YELSDDKAVL HGSDITLDNV DSPTFIAMRQ
     RDFNMEMTST VKLTGSAGAV GGLTVLITEN EHYDILLEKS ERGCKAVLML NIGGIKHRQN
     ELPIGGDSAK LTIKADNYGY SFFCGEVQLG YGSTKYLSSE VAEGFTGVMT GLYAARGTAE
     FTGFECRYN
//