ID R5N4W1_9FIRM Unreviewed; 690 AA.
AC R5N4W1;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE SubName: Full=2-enoate reductase {ECO:0000313|EMBL:CCY92611.1};
GN ORFNames=BN519_01561 {ECO:0000313|EMBL:CCY92611.1};
OS Eubacterium sp. CAG:180.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Eubacterium.
OX NCBI_TaxID=1262882 {ECO:0000313|EMBL:CCY92611.1, ECO:0000313|Proteomes:UP000018411};
RN [1] {ECO:0000313|EMBL:CCY92611.1, ECO:0000313|Proteomes:UP000018411}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:180 {ECO:0000313|Proteomes:UP000018411};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: In the N-terminal section; belongs to the NADH:flavin
CC oxidoreductase/NADH oxidase family. {ECO:0000256|ARBA:ARBA00011048}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCY92611.1}.
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DR EMBL; CAZC010000070; CCY92611.1; -; Genomic_DNA.
DR AlphaFoldDB; R5N4W1; -.
DR STRING; 1262882.BN519_01561; -.
DR Proteomes; UP000018411; Unassembled WGS sequence.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR CDD; cd02803; OYE_like_FMN_family; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR001155; OxRdtase_FMN_N.
DR PANTHER; PTHR42917; 2,4-DIENOYL-COA REDUCTASE; 1.
DR PANTHER; PTHR42917:SF2; 2,4-DIENOYL-COA REDUCTASE [(2E)-ENOYL-COA-PRODUCING]; 1.
DR Pfam; PF00724; Oxidored_FMN; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00469; PNDRDTASEII.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR SUPFAM; SSF51395; FMN-linked oxidoreductases; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023014};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00023014};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Reference proteome {ECO:0000313|Proteomes:UP000018411}.
FT DOMAIN 6..381
FT /note="NADH:flavin oxidoreductase/NADH oxidase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00724"
FT DOMAIN 428..661
FT /note="FAD/NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF07992"
SQ SEQUENCE 690 AA; 76181 MW; 792734A8548A80FD CRC64;
MEYKMLFTPM NIGTVQIKNR VVMPPMMLGF GQFNGCPTKE MMDYYEERAI GGAGLIITEI
TRVDDFSGAS AFAQLAISHD YHIKPLAEMI SRVHSHGCKM FIQLHHPGRQ NLGLLMGTVP
VSIGAERIMG KLYDKMFYKV APAGKMLMQK DLVLRTFSPS KCDRAYSAES LNREMSVKEI
KKIISEFING AVRAQKAGAD GVELHAAHGY LIQQFLSPYT NTRNDEYGGS FEKRMKFLID
IINGIKAACG NDFPIVVRLS VDECYSMIGK PEIGYKLEDG VRMAKVLEKE GIDAIDVSCA
GYDTYNYWLE PTSFEPGWRK YMAAAVKKEV KIPVLAANLI RSPKQAEEQL EEGIQDFVSL
GRTHIADPHW VNKVKAGKED EIKRCICCLY CMESMQKNAY KGTHAHCSVN PKLGKESEHP
IANGNGRTVV IVGAGPAGLM SAEVLAKRGF KPIVLEKNAF PGGQLQLANK PPLKEKINWC
IEDLVTNATH YGAEIKYNVI ADESIIKSYN PYAVIIATGG KAIKPKSIKG SDKDNVFTTT
DILDGSVVLK NKKVAVIGSG MTGLETSEML AENGNDITVV EMADSIAPGT WMQHIDDCMP
RLKAHGVKFI VGHKLTEITD RGIELNKVDT ENILLVDADY VVLSLGVRPE NKLYDDINGK
LKNVYLIGDA EKTGRIADAT SQAYELCKNI
//
