ID   R5S035_9FIRM            Unreviewed;      1038 AA.
AC   R5S035;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   24-JAN-2024, entry version 27.
DE   SubName: Full=Restriction endonuclease {ECO:0000313|EMBL:CCZ46835.1};
GN   ORFNames=BN483_00434 {ECO:0000313|EMBL:CCZ46835.1};
OS   Firmicutes bacterium CAG:129.
OC   Bacteria; Bacillota.
OX   NCBI_TaxID=1263003 {ECO:0000313|EMBL:CCZ46835.1, ECO:0000313|Proteomes:UP000018410};
RN   [1] {ECO:0000313|EMBL:CCZ46835.1, ECO:0000313|Proteomes:UP000018410}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:129 {ECO:0000313|Proteomes:UP000018410};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CCZ46835.1}.
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DR   EMBL; CBAB010000272; CCZ46835.1; -; Genomic_DNA.
DR   AlphaFoldDB; R5S035; -.
DR   Proteomes; UP000018410; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015668; F:type III site-specific deoxyribonuclease activity; IEA:InterPro.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR045572; RE_endonuc_C.
DR   PANTHER; PTHR47396:SF1; ATP-DEPENDENT HELICASE IRC3-RELATED; 1.
DR   PANTHER; PTHR47396; TYPE I RESTRICTION ENZYME ECOKI R PROTEIN; 1.
DR   Pfam; PF19778; RE_endonuc; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE   4: Predicted;
KW   Endonuclease {ECO:0000313|EMBL:CCZ46835.1};
KW   Hydrolase {ECO:0000313|EMBL:CCZ46835.1};
KW   Nuclease {ECO:0000313|EMBL:CCZ46835.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018410}.
FT   DOMAIN          107..272
FT                   /note="Helicase/UvrB N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF04851"
FT   DOMAIN          922..1026
FT                   /note="Type III restriction enzyme C-terminal endonuclease"
FT                   /evidence="ECO:0000259|Pfam:PF19778"
SQ   SEQUENCE   1038 AA;  118993 MW;  8DFECAAC5B9EA0D3 CRC64;
     MKFNFKIQQY QTDAVDAVVR VFQGQVFHDK ISYIRDRGKV KVEPYQMTLG MSDEELEIVD
     PLNDTGYKNE AVELSDEQLL YNIQALQSQN NIKLSASLVK DLGRCSLDIE METGTGKTYV
     YIKTMFELNK KYGWSKFIVV VPSIAIREGV KKSFEITADH FMEYYGKKAR FFIYNSSNLN
     QLDNFSSSSG INVMIINTQA FASSLKEDGK SKEARIIYSK RDEFGSRRPI DVIKANRPII
     ILDEPQKMGG DVTQKALKNF NPLFSLNYSA THAKHHNLVY VLDALDAFNK RLVKKIEVKG
     FEVRNFRGTD SYLYLEQIIL SSKKPPMAKI ELEIGYNKSI NRETRILSVG DDLYFVSQEM
     EQYKGYTISE IDPLRGTVTF TNGEVIKAGD VVGDVSEKDM RRIQIRETIL SHFEKEEKLF
     NMGIKCLSLF FIDEVAKYRQ YDENGDEVLG EYGVMFEQEY LAILNEYITM FDTPYQKYLK
     STCSDVSRVH KGYFSIDKKT GRSVDSQLKR GSEFSDDISA YDLILKNKER LLSFDEPTRF
     IFSHSALREG WDNPNVFQIC TLKHSDSNTA KRQEVGRGLR LCVNQDGNRM DAQSCGDSVH
     EINTLTVVAS ESYKTFVTDL QSDIKTVLYD RPTVATSEYF KGKYVKVDDV PTLIDDEKAN
     AIEFYLIQNG YVDMKRKVTD KYRQDVKNGT VAELPDELKP MTDGIHTLIQ AVYDDSVLKD
     MFSDGHETKV KDNPLNENFA KKEFQALWRE INHKYAYTVD FDSAELIRNA IAHIDKKLFV
     SELQYTTTIG RQKAEMNEYE IERGDSFTGE KTRTQTLKHA EASQIKYDLI GKIAEGTVLT
     RRTVSAILQG IRVDKLYMFR NNPEEFISKV IRLINEQKAT MIVEHISYDT IEGEYDSSIF
     TAEKATQSFD KAFLAKKAIQ DYVFTDGSAD KSIERKFAED LDAAEEVCVY AKLPRTFQIP
     TPVGNYSPDW AIAFYEGTVK HIFFIAETKG TMESLELRPI EQAKISCAKK LFNEMSTSNV
     VYHDVDSYRS LLSIMNSI
//