ID R5SF25_9CLOT Unreviewed; 1348 AA.
AC R5SF25;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00013303, ECO:0000256|RuleBase:RU361154};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230, ECO:0000256|RuleBase:RU361154};
GN ORFNames=BN771_00487 {ECO:0000313|EMBL:CCZ51748.1};
OS Clostridium sp. CAG:75.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1262836 {ECO:0000313|EMBL:CCZ51748.1, ECO:0000313|Proteomes:UP000018032};
RN [1] {ECO:0000313|EMBL:CCZ51748.1, ECO:0000313|Proteomes:UP000018032}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:75 {ECO:0000313|Proteomes:UP000018032};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU361154};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401, ECO:0000256|RuleBase:RU361154}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCZ51748.1}.
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DR EMBL; CBAE010000025; CCZ51748.1; -; Genomic_DNA.
DR Proteomes; UP000018032; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 3.
DR InterPro; IPR004199; B-gal_small/dom_5.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR023230; Glyco_hydro_2_CS.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032312; LacZ_4.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF02929; Bgal_small_N; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF16353; LacZ_4; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SMART; SM01038; Bgal_small_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361154};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361154};
KW Reference proteome {ECO:0000313|Proteomes:UP000018032}.
FT DOMAIN 870..1141
FT /note="Beta galactosidase small chain/"
FT /evidence="ECO:0000259|SMART:SM01038"
FT REGION 1175..1253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1198..1242
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1348 AA; 151140 MW; 53933656F23E6B46 CRC64;
MKKIWGKRIG TGVLAISLML SSLPTQLSIA LPHKAKAADT EFKSEAFDYG NLPDSTAAKI
TRKSLTGREW TGEDNNLDIT SVNTLPDSSN LVPYADIASA YAGAKDYARD KSSYYQLLTG
EGQDWDLTVF DNPEKAEAVG AFQNTNYKMD AKDGWKKVTL PASWTSYGFD HSIYTNSAMP
FEESTEFPLA PTKKNPVGLY RKTFTVKDSM LQKNGKVYIT LGGVESAYYL YINGKEIGYS
EDSYDPHTFD ITDALNDPGK ENVLAVKVYK FCDGTWMEDQ DMIYDGGIFR DVYLTSTPTV
HVQDYTLATD LSDDYSTADL KVKMQTINDS ATESSNMAAQ LNLYDDQGNL CATTQEDIAA
IASGKQTETS LQMQITDPKL WDSDHPNLYT MVLSLYDKSS KLHYESVSQN VGFRKLTFTS
TKVTNDGKYN NATDHYDTVK LNGKRLLIKG VNRHDTDPET GKYISKKVYE KDIMLMKQNN
INAIRTSHYP NDDYMYYLCD KYGLYLMCES NNESHALITD EDAIATLEKA AMTRQTASYE
RFKNTTSNLF WSIGNESSQG WTQRDGNYAN GAFAHMVQYF KDRDDSRMLH YEGMSGGDKG
STAIDMVSHM YYTPDSIIGY GTSKSQMPFL LNEYDHAMGN AVGNLSDYWD VIRKYDSMLG
GFIWDWVDQS RKVKIGEKDW NYYSSKDAHQ SGLNQLDGYF LGYGGDWGDT GGDENFCQNG
LVSADRDPQP ELKEVKYQYQ SFWFKSDQDK LTNNELTVSN ESISAKLSEY DVTWELQEND
QIISQGTMND EVLPKEEKKI TVPYVMPEQL KSGADYYLNI HVKTKEDSDW AKAGYEVAYA
QFTIDAKSTK VAHTLNGKNV QIKKQSHYFI VSGKDFRFKL NLDTGLLESY YYKDQLLMKE
GPKPNISRAK LDNDSSHFKD IMSYLTLDGE PTVGKSADGN YMLTTKWNSS YLLDSKTKTP
GTIEMSYLIE DTGAVTVRMK LDFTKTKVKK FIKVGTRLSL AKGTENVSWY GNGDDESYCD
RQTYTRVGAY TSTVNKMFYP FAKPQDCGNL TGVKWIRLDN ETNGTGMLIC GNEDVNASAL
HFMTEQLDKA KHVNELKPLT KTFVTVDAAV SGTGNASCGF DTLDPYLVKN NKVYDYRYTL
VPVSNTDDSM TVGKEYRDQT FDMDQVSYEK VDPVVTDGEP AADPVDEDEA ILNPPKEDDN
NCGNNSGSNT GNNTGNNTGN TGNNNTGNNG GSINNAGKNP GTKQTAKAPK KVTKIKVKKL
KKALSLRWKS QKNVTYRIAY STSKKKLSKI KNGKLKAVKG TKVIKVTSAK KTIKKLKKSK
KYYLKICAVS KNRKNIGKWS GVISAKTK
//
