ID R6D3Q4_9FIRM Unreviewed; 1186 AA.
AC R6D3Q4;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=BN516_00925 {ECO:0000313|EMBL:CDA82375.1};
OS Firmicutes bacterium CAG:176.
OC Bacteria; Bacillota.
OX NCBI_TaxID=1263007 {ECO:0000313|EMBL:CDA82375.1, ECO:0000313|Proteomes:UP000017932};
RN [1] {ECO:0000313|EMBL:CDA82375.1, ECO:0000313|Proteomes:UP000017932}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:176 {ECO:0000313|Proteomes:UP000017932};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDA82375.1}.
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DR EMBL; CBCO010000192; CDA82375.1; -; Genomic_DNA.
DR AlphaFoldDB; R6D3Q4; -.
DR Proteomes; UP000017932; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006302; P:double-strand break repair; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014152; AddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR02785; addA_Gpos; 1.
DR PANTHER; PTHR11070:SF48; ATP-DEPENDENT HELICASE_NUCLEASE SUBUNIT A; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000017932}.
FT DOMAIN 4..476
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 477..777
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 25..32
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1186 AA; 132227 MW; D131FD37E7440564 CRC64;
MADIQLTAQQ RGAVEDRGGS LLVSAAAGSG KTKVLVERVF AYLTEEHCHI DDFLIITFTR
AAAAELRTKL AAELAKRVAQ DPENSHLRQQ MFRVYQADIK TVDGFCAGLL REHIHLLEPV
DGRSLTPDFR ILDETEAGVL KQRALEDALE AFYQRIEQGD EGCRALAETL GFGRDDRALA
ALVPELHGKL QSHPYPDKWL EKAAESWANL PERLGDSVYG RTIMEDTVRR ASYWAGRLER
AVKEMEDCES VYNAYGDRFL ETAAQLREYA AAAREGWDAM GCVHPAFRRM GVVKGDECAG
AKDAARAVLE KCRDALKKLS APYQTAETEL LGDLQVIAPA MRALLALTGD FDKRFRAEKV
RRNAMDFSDQ EHYAVQMLCR QDGTPTELGE QVSRRYREIM VDEYQDTNEV QNCIFRAVSR
QGENIFAVGD VKQSIYRFRL AEPGIFLEKY RTYADADHAA LGAPRRRVLS RNFRSRREVL
AAANFVFAAL MSREMGELDY TEEQHLHFGA EYYPEVPRET EFHYLAVEDT PEQRFDRAEA
EARFVAKRIR RMLDEGFTVR GEDGTMRPAA PEDIVILMRS PSARQTVFAA ALAREGIPCD
GGESEDFFSA MEVAVVLSLL EVVDNPRQDV PLIAVLRSPL VGMSADRLAE IRAVQREGDY
YEALRQDEGE DAQAFLSLLH ELRQVARELS ADKLLWYIYD RCRVQAIFGA MEDGTARQAR
LRALYDYIRR LVQGGRTSLF DCVRQVRQLL ESGDAPAITA AGAAGGVRLM SVHKSKGLEF
PVVFLADLNR SFNRQDLDRP VLVHPQLGVG AERVEVERRL RYDTVSKTAL ALTLEREAKA
EELRILYVAM TRAQEKLIMV CSRKNPEKHL KELCALAELP APPEAVAAVN CPGDWLLLAL
LSTYQAGVFH DYTGVRPAVL YDGPEGLALR LHRISGEEDG GAAMPAEEPE ARAPDPQPDE
AALAFRYSHR AATVTPSKVT ATQLKGRAID QEIAEGTLPA RRTAAPEKPR FLQEKRGLTG
AERGTAMHLA MQFLPLDTPA VPEAVTAFAE RLTARRLLTP EQAAALDIPA LARFLASSLA
ERIRTAPQVW REYRFALLTD AGIYDVAAAG EEILLQGVAD CVFETTAGLT VVDFKTDRVT
AAEASQRAEA YRPQLDAYAG ALSRILEKPV TERILYFFAC GEEISL
//
