ID R6EWW0_9BACT Unreviewed; 856 AA.
AC R6EWW0;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=Putative exo-poly-alpha-D-galacturonosidase {ECO:0000313|EMBL:CDA94397.1};
GN ORFNames=BN487_00955 {ECO:0000313|EMBL:CDA94397.1};
OS Prevotella sp. CAG:1320.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Prevotellaceae;
OC Prevotella.
OX NCBI_TaxID=1262922 {ECO:0000313|EMBL:CDA94397.1, ECO:0000313|Proteomes:UP000018111};
RN [1] {ECO:0000313|EMBL:CDA94397.1, ECO:0000313|Proteomes:UP000018111}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:1320 {ECO:0000313|Proteomes:UP000018111};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-
CC galactosidic residues, producing free D-galactose.; EC=3.2.1.n1;
CC Evidence={ECO:0000256|ARBA:ARBA00001271};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family.
CC {ECO:0000256|ARBA:ARBA00008834, ECO:0000256|RuleBase:RU361169}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDA94397.1}.
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DR EMBL; CBCU010000039; CDA94397.1; -; Genomic_DNA.
DR AlphaFoldDB; R6EWW0; -.
DR STRING; 1262922.BN487_00955; -.
DR Proteomes; UP000018111; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0004650; F:polygalacturonase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 2.160.20.10; Single-stranded right-handed beta-helix, Pectin lyase-like; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR000743; Glyco_hydro_28.
DR InterPro; IPR010905; Glyco_hydro_88.
DR InterPro; IPR006626; PbH1.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR PANTHER; PTHR31339; PECTIN LYASE-RELATED; 1.
DR PANTHER; PTHR31339:SF9; PLASMIN AND FIBRONECTIN-BINDING PROTEIN A; 1.
DR Pfam; PF00295; Glyco_hydro_28; 1.
DR Pfam; PF07470; Glyco_hydro_88; 1.
DR SMART; SM00710; PbH1; 5.
DR SUPFAM; SSF51126; Pectin lyase-like; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00502; POLYGALACTURONASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361169};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361169};
KW Reference proteome {ECO:0000313|Proteomes:UP000018111};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..856
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004417216"
SQ SEQUENCE 856 AA; 97027 MW; C91C08A4EF5C046A CRC64;
MRKLKTLLLA MLLPVCALAS GWDDNEYKQI EASVQQPQID TKRYPVTDYG AKTTATAAQN
QKAINKAISA ASSKGGGQVI IPAGTWKTGA ITLRSKVNLV VEEGATLQFV FDTDLYPLVK
TSWEGIGCWN YQPCIYANGA TDIAITGKGT INGGGSNDTW WKMCGKDRFG YVEGETKEAQ
NLGSRARLLR YAEDGVEWDE RKFGKGQGLR PQLINFLYCD RILIKDVKLY DSPFWVIHPL
LSKNITVDGV YIWNEGPNGD GCDPEGCENV LIQNCVFHTG DDCIAIKSGR NNDGRLWNQP
SRNIIIRNCK MEDGHGGVVI GSEISGGCEN VYAEDCHMDS PELERVLRIK TNNCRGGLIQ
NINMRNVTVG QCKEAVLKIN LDYEPKEECY RGFEPTVRNV SMENVTCQKS EYGVLVIGRN
NVENVYDINV KDCEFNGVHK KPVQITGKTR DIKFDNLHIN GSLVLNEGEA PFERYSEWLA
YSEMKRTPLS YLLDFAKKPR WSYVMGIELE GMLDTYEAYG GDSIMRYLEA YPAKMIDEKG
NITGYKYEDF NLDNVRTAKF ILRMHNLEPA KNTEKALKTL FKQLEKQPRT KEGVYWHKAI
YANQVWLDGI FMGLPFYTAY ATQTMKPKKA VKYLDDAVDQ IIKTDQRTYD KKTRLWKHAW
DETREQFWAD KTTGQSQHTW ARALGWYTMA MIECLEAMPQ DYARRGEVVA LLRKAMKSVV
DYQDPETGVW YDVMDVKDPR NYLESTASCM FAYVLLKGYR LGYLDESYRD AGVKAYQGII
NEFIKVNDDK TISLTQCCAV SGLGPGPGPY VKKPNFKRDG SFDYYMSEPI RDNDAKGIGP
FLWASLEMER QGLPTS
//
