ID R6H030_9FIRM Unreviewed; 635 AA.
AC R6H030;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00012555};
DE EC=2.4.1.129 {ECO:0000256|ARBA:ARBA00012555};
GN ORFNames=BN704_01005 {ECO:0000313|EMBL:CDB26841.1};
OS Firmicutes bacterium CAG:552.
OC Bacteria; Bacillota.
OX NCBI_TaxID=1263029 {ECO:0000313|EMBL:CDB26841.1, ECO:0000313|Proteomes:UP000018403};
RN [1] {ECO:0000313|EMBL:CDB26841.1, ECO:0000313|Proteomes:UP000018403}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:552 {ECO:0000313|Proteomes:UP000018403};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.1.129;
CC Evidence={ECO:0000256|ARBA:ARBA00023988};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDB26841.1}.
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DR EMBL; CBDH010000088; CDB26841.1; -; Genomic_DNA.
DR AlphaFoldDB; R6H030; -.
DR STRING; 1263029.BN704_01005; -.
DR Proteomes; UP000018403; Unassembled WGS sequence.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009002; F:serine-type D-Ala-D-Ala carboxypeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF32; PENICILLIN-BINDING PROTEIN 2A; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 4: Predicted;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000018403}.
FT DOMAIN 61..220
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 299..552
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
SQ SEQUENCE 635 AA; 70838 MW; C89DE3B80C62A49D CRC64;
MKKFLRYFFA FVLITAVVSL WAITEPFIAM LGYGDMNIGV LTKNDNYTIV SKDYLFAGKS
IDVTELNKNT VNAFLAAEDK RFYEHEGVDY ARTVAAAVKD IKEKKFAQGG STITQQLAKN
MFLSSEKTLK RKIKEIRFAK AIERKLTKNQ ILTAYLNNLY FGNGIYGIQS AANSFFNKNA
SNLTTKESAI LAATINNPTV FSPYKERNEV RVKTILKRMK DCGYISDIEY ELSLSEKTTL
MPENGESKYW KSALEEAKNI LGVSMETLKT GGYKIVCNYD KNISIAAKNL LPKDTKTRIL
VLKNNNGQII AYESNCDVGI YAKRSPASTI KPLVCYAPML ENEKILPCSP ILDESVDFDG
YAPKNYKGKY NGWVSCGFAL KESLNVPAVK LLEEYGVEKG KAFLKRFGIE TEKNEGLALA
LGGMKYGVEI PDLAAAYATF ANDGMYTEAK IVSEIQKNGI TVYKRYSAPT QIIKSTTAYY
INEMLNDCAS NGTAKSLSAT KFNLAAKTGT AGTGEVNTDS YCVAYNTDYT VIVHAIDSAV
SGGGLPTEIA KSLFMTDINS EKCFNIPDSI KKIYIDADEY YQNHRLVKSA TTDKLKDKIL
ISASIDFPIE QKKTNLAEIL SPYFDNFSRF YRFFY
//
