ID R6MKB3_9FIRM Unreviewed; 556 AA.
AC R6MKB3;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=rhamnogalacturonan endolyase {ECO:0000256|ARBA:ARBA00012437};
DE EC=4.2.2.23 {ECO:0000256|ARBA:ARBA00012437};
GN ORFNames=BN647_02186 {ECO:0000313|EMBL:CDB99105.1};
OS Firmicutes bacterium CAG:41.
OC Bacteria; Bacillota.
OX NCBI_TaxID=1263021 {ECO:0000313|EMBL:CDB99105.1, ECO:0000313|Proteomes:UP000018139};
RN [1] {ECO:0000313|EMBL:CDB99105.1, ECO:0000313|Proteomes:UP000018139}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:41 {ECO:0000313|Proteomes:UP000018139};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-
CC (1->4)-alpha-D-galactopyranosyluronic acid bonds of
CC rhamnogalacturonan I domains in ramified hairy regions of pectin
CC leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-
CC unsaturated D-galactopyranosyluronic acid at the non-reducing end.;
CC EC=4.2.2.23; Evidence={ECO:0000256|ARBA:ARBA00001324};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 4 family.
CC {ECO:0000256|ARBA:ARBA00010418}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDB99105.1}.
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DR EMBL; CBEM010000177; CDB99105.1; -; Genomic_DNA.
DR AlphaFoldDB; R6MKB3; -.
DR Proteomes; UP000018139; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0102210; F:rhamnogalacturonan endolyase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR CDD; cd10317; RGL4_C; 1.
DR CDD; cd10316; RGL4_M; 1.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.40.1120; Carboxypeptidase-like, regulatory domain; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR029413; RG-lyase_II.
DR InterPro; IPR029411; RG-lyase_III.
DR InterPro; IPR016590; Rhamnogalacturonase_B.
DR InterPro; IPR015364; RhgB_N.
DR PANTHER; PTHR36574; RHAMNOGALACTURONATE LYASE-RELATED; 1.
DR PANTHER; PTHR36574:SF1; RHAMNOGALACTURONATE LYASE-RELATED; 1.
DR Pfam; PF14683; CBM-like; 1.
DR Pfam; PF14686; fn3_3; 1.
DR Pfam; PF09284; RhgB_N; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Reference proteome {ECO:0000313|Proteomes:UP000018139};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 39..291
FT /note="Rhamnogalacturonase B N-terminal"
FT /evidence="ECO:0000259|Pfam:PF09284"
FT DOMAIN 298..371
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14686"
FT DOMAIN 388..555
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14683"
SQ SEQUENCE 556 AA; 61479 MW; 261A8C8352643E7B CRC64;
MKNESQLKSS KRGVLLRLAI FMALMISAFI IPSSVLADFG YTEDSTNYTI DTGANLVFKV
KRSNGDISSL IYNGTDYNGY TNKNSHVETG LGQSDVTISQ PSSSVIMVKV VYGTLEQYYV
ARKGENNIYM FTYIADDSVT VTRYIVRLKP SLFPVLNTSN SWYSSYSTLE AKDIFTDTST
GYTYSKHYSD TRVMDYNYTG ISNGNVGAYI VRSNHEKASG GPFYRSLIRD NTDVAVNLYE
ILYYGMAQID VKRYGLQGPY VLAFTDGGEP SSKLYAGNLK TDWIDSLGIH GWVGSSGRGR
VAGVGIKNMK SDYEYVVGFS NDEAQYWTKA SDSNGYFSCT NMLPGTYTMT IYKNELAVYT
TDVTVTAGGT KILNSITITD DPSDNDVTWR IGDWDGTPLE FKNGSLMTKM HPADSRVESW
KGNYIVGTSD ASSFPCYIFK DVNNGLIVYF RLNSEQAAKD HTLKIGITGS YIGGRIQPSV
NSWTSKLPSA SNQPGTRLMT IGTYRGNNYT YSFTVPASAF NTNTRDWNQL KLNVISGSNG
SDYLSPAVSI DCIELE
//
