ID R6N757_9FIRM Unreviewed; 1178 AA.
AC R6N757;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=BN531_01253 {ECO:0000313|EMBL:CDC02066.1};
OS Eubacterium sp. CAG:202.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Eubacterium.
OX NCBI_TaxID=1262884 {ECO:0000313|EMBL:CDC02066.1, ECO:0000313|Proteomes:UP000017996};
RN [1] {ECO:0000313|EMBL:CDC02066.1, ECO:0000313|Proteomes:UP000017996}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:202 {ECO:0000313|Proteomes:UP000017996};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDC02066.1}.
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DR EMBL; CBEO010000062; CDC02066.1; -; Genomic_DNA.
DR AlphaFoldDB; R6N757; -.
DR STRING; 1262884.BN531_01253; -.
DR Proteomes; UP000017996; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006302; P:double-strand break repair; IEA:InterPro.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 6.10.250.2380; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014152; AddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR02785; addA_Gpos; 1.
DR PANTHER; PTHR11070:SF48; ATP-DEPENDENT HELICASE_NUCLEASE SUBUNIT A; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000017996}.
FT DOMAIN 2..472
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 473..770
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 23..30
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1178 AA; 132883 MW; 5636433442334378 CRC64;
MTTFTDAQTD AINAQGGSVL VSAAAGSGKT TVLIERIIRM ITRRENPIDV DRLLIVTFTR
AAASEMKERL AKAINSLLEN DPYNSLLLRQ RQLMYNTSIC TIDSFCGSVV REYFHILGVQ
KDFRLADENE IKVIGSEALE SVLEDFYREN TSDFTALVDA FANKNGDDNL RAAVLKISDF
LATQPFGEKW LDDMLLKYSP EIPVEQSLFG KILIKYARSM VDHSLLITIN SIKLLDGDEK
LRDANLPLLL DDKAYFEKLS ERFEDGSWDE IKRFAETFVK GRLKTPKGYK DDPIKISIAN
SRDDVKKSVE SICNIFMWSS EEIKNEIGYL SKTVAELFEV VKAYLSKFSE MKYAKNILSF
SDVELLTVKL LAEPCGDGYT KTEQAKEISK RFDQIMVDEF QDVNDVQDLI FKCVSTDENN
MFVVGDVKQS IYGFRQAKPE IFIDRKDKYN KYDRENEIFP AAIILDMNFR SRKEVCSAVN
FIFKNLMTKT SAKMDYGTDE YLNVGASYPP SDGCNFEVDY IQYESSDESV ENEAVFIANK
ILKMRAEGFT VGKDKKPLEY GDCAILLRST KNTATAFVNI LNSLGVPASS EIKESFFDAN
EIKVMLNFLR CIDNPSLDIP LLSVMCSPVY SFTVDELAKI KGNNRHTNLY SAVAAYAKND
SRTAEFLREL NLLKTYSCTC SVDELLGRIY ELTSFSAVIS AIDGSKGIKN LNLLREYARG
FESGGYRGLS AFITYMDKLI ENGADLESSS GANGETENTV KVISIHKSKG LEYPVCFIAC
SSKKFNKTDL SADVLIDSKA GLGIRRVDGF CRYNTLPRMA VGVEISENEI AEEMRVLYVA
LTRAKEKLIV ISSQKDPKDY LVKLNSKLAG GVIDPYAVMK ARSISDWVSM CALVHPLHNE
LRMKLQIGES DLMGTASEEL PWQINFISPS DFVSETDERS NDVKNNSDNE TYDNFVIDLL
KERTGFVYRN QAVLKIPQKV SASELAHNEG DSFDKIFLKP AFMSKGNTSA VEKGTAHHKF
LQFCNFKDAK NNLQAELDRL LSENILTQEQ FDAINKDEIQ KFLDGSLAKR ITASPLIMRE
ERFTVNIKAG MLDSTLEGDS ADTRVVMQGA VDLIFKEDNE LVVVDYKTDR VRDISRLKEL
YAKQLNLYKL AAEEFTDYKV KECIIYSIHL NDYISVDC
//
