ID   R6SCR5_9FIRM            Unreviewed;       510 AA.
AC   R6SCR5;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   24-JAN-2024, entry version 25.
DE   SubName: Full=Xylosidase/arabinosidase {ECO:0000313|EMBL:CDC44397.1};
GN   ORFNames=BN652_00747 {ECO:0000313|EMBL:CDC44397.1};
OS   Firmicutes bacterium CAG:424.
OC   Bacteria; Bacillota.
OX   NCBI_TaxID=1263022 {ECO:0000313|EMBL:CDC44397.1, ECO:0000313|Proteomes:UP000018167};
RN   [1] {ECO:0000313|EMBL:CDC44397.1, ECO:0000313|Proteomes:UP000018167}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:424 {ECO:0000313|Proteomes:UP000018167};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDC44397.1}.
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DR   EMBL; CBFI010000044; CDC44397.1; -; Genomic_DNA.
DR   AlphaFoldDB; R6SCR5; -.
DR   Proteomes; UP000018167; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd18617; GH43_XynB-like; 1.
DR   Gene3D; 2.60.120.200; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR041542; GH43_C2.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR   PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR   Pfam; PF17851; GH43_C2; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR   SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018167}.
FT   DOMAIN          316..496
FT                   /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT                   /evidence="ECO:0000259|Pfam:PF17851"
FT   ACT_SITE        14
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        175
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            120
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   510 AA;  57856 MW;  1BC4460D4E2B222D CRC64;
     MKYRNPIISG YNPDPSICRV GDDYYIVNSS FEYFPGVPVY HSKNLVNWEL IGNCLDRKSQ
     LLLEKCKPSG GIYAPTLRYH DGTFFMTTTN VSHKGNFIVH TKDIKKGWSE PVWIDQGGID
     PSLLFDDDGT VYFLSTATDE QGEAGIFMCE INPFTGEKLT ESVVISRGCG GRYAEGPHLY
     KWFGKYYLML AEGGTEYGHM ETMLRSDFPY GPYEACPHNP ILTHRDDMRE EIYCTGHADM
     MEDHNGNWWM VCLAIRPCEE KSNRVLLHNL GRETFLAPVV WDEEGWPVVG DKGLISLEMD
     GPLPGPEPGK VNRDFSDDFS DETFSTSYNF LRNPNMEHYK RDTGNAALVL QGTEITLNDM
     DSPTWLGIRQ KGFETVSSVE VCLLEAENNV RAGLTAFYTE SYHYEIYITR ENGVSKVCLA
     KHIHDIYVVT AEKEISDAEN IILRVESDKK YYRFAFSVDG KVFTELGTGL VVGLCTEGTK
     YMTFTGTYIG MFAENGIARF KNFKINVKDY
//