ID R6SKI1_9LACO Unreviewed; 1306 AA.
AC R6SKI1;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 24-JAN-2024, entry version 40.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=BN628_01544 {ECO:0000313|EMBL:CDC58039.1};
OS Ligilactobacillus ruminis CAG:367.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Ligilactobacillus.
OX NCBI_TaxID=1263085 {ECO:0000313|EMBL:CDC58039.1, ECO:0000313|Proteomes:UP000018129};
RN [1] {ECO:0000313|EMBL:CDC58039.1, ECO:0000313|Proteomes:UP000018129}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:367 {ECO:0000313|Proteomes:UP000018129};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDC58039.1}.
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DR EMBL; CBFO010000087; CDC58039.1; -; Genomic_DNA.
DR Proteomes; UP000018129; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0051060; F:pullulanase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd10315; CBM41_pullulanase; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 2.60.40.1110; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR005323; CBM41_pullulanase.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR022263; KxYKxGKxW.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR03715; KxYKxGKxW; 1.
DR NCBIfam; TIGR01167; LPXTG_anchor; 1.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF19258; KxYKxGKxW_sig; 1.
DR Pfam; PF03714; PUD; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 1.
DR PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE 3: Inferred from homology;
KW Cell wall {ECO:0000256|ARBA:ARBA00022512};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Peptidoglycan-anchor {ECO:0000256|ARBA:ARBA00023088};
KW Secreted {ECO:0000256|ARBA:ARBA00022512};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 20..36
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1281..1299
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1271..1306
FT /note="Gram-positive cocci surface proteins LPxTG"
FT /evidence="ECO:0000259|PROSITE:PS50847"
FT REGION 58..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1182..1277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..85
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..141
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 150..187
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1182..1227
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1235..1258
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1306 AA; 140721 MW; ABF01C25E7388415 CRC64;
MHNQDVVQEN WRMWKHGKQW LYASSAVLAM GIYMGLSTTT VQADTLSNTQ TTTTAVQSVA
KPATATTTTT GTTNAEQSGT VAETDQVPSI DPAADQPTKT DVAISQTPAA TSTLTGEKDL
QSSLETKTNQ STSSTKSVAV PEVAKTNPVV TSEKAKTATP KNSQVETPAP VTQPVSAPAQ
VDTKTETTAP VPVKDTTKVI IHYQGDSSKW APYVWGLKPN GNGQQYDWTG TDDFGAYAEI
DMDQNYQQLG VLIKGVDSWD KDGNGADRTV TVDANGKGEV WYRAGSDDAQ DVTPTYHDTT
INVHYHDKNQ SAVNHYRVWT DKAGEAGAQT VDLNQTDANG NLLGTVQLTA ADFTTVYVQP
VGADELTRAF TPVASDAATD IYVVAGDQQP YYTASFALQQ EMVTSAAMQT PTEVTVTIGK
ALTAAEARQQ LSVAGHTVKD VTAIAPDATG RSKQFKLTTT TDLDIMTTNS VSFNGNAKVM
DIGDYVRSQA FDNEYYYDGD DLGVTYSKQR TQIKLWAPTA NQIELRLYQQ HTADAAVTKT
IAMQRGAKGV WTAVLPGDYQ GWAYDYQLHF GDGSVTTTED PYSKAVTVNG NRSVIADVDA
VKPADFDRLP SFSDPTDAVI YETSVRDFTK DPNSGVTAKG KFTGMVESGT HTASGQATGL
DYLKDLGVTH VQLMPMYDFA SIDETSADPS YNWGYDPKNY NVPEGTYSSD ASDPTARVLE
MKEMVNGYHK AGIRVVMDVV YNHVYSMDEQ AFQKVVPGYY FQYDQGGHPT SGSGVGNDVA
SERRMVRKYI LDSVKYWATE YNIDGFRFDL MGILDVDTMN AIRAELNAID PGILVYGEGW
DMRATNYDIG AGQYNADKLD QHIGFFSGDI RDAIRGAEFG GISKGLVEGN AQEDDYDQHA
QAFIAGFLGG QDYANAVPAH PYQSPSQTLN YVACHDNRTL YDMLTALMPN ESQANLIKRD
KLATSMAFLA EGVPFVYGGQ ESLRTKGGDE NSYKSPDAVN QIDWDRVQDN QELVDYFKQL
VQLRKDESAF RLTNYADIDK TVKVLQDGKN GVFAFEYLAG GHKLYVLFNV NDSAQVFDAV
NLTNGRVLLG SDAVTLGTVT NLAALSTLVV REDLPQTVTI NYQDDNGQLV KTTTVTLTAA
GTAYTLTAPA GYHLTGDQTT VSYDFDNDGT ARIVTVLVAK MTTDNGQPGG QPSGGNGQTG
DHHGKENGQT GATTQPGGGS TTTTAEDQRA GEVEKPTIPT TTANQATSSA TKATLTPTTA
AKAGRAAATG LPQTGERAGQ SASLLGLLGI MLAGLMSLVK PQRKRN
//
