ID   R7B4K5_9CLOT            Unreviewed;       563 AA.
AC   R7B4K5;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 30.
DE   SubName: Full=Subtilase family protein {ECO:0000313|EMBL:CDD59461.1};
GN   ORFNames=BN653_00118 {ECO:0000313|EMBL:CDD59461.1};
OS   Clostridium sp. CAG:43.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1262805 {ECO:0000313|EMBL:CDD59461.1, ECO:0000313|Proteomes:UP000018284};
RN   [1] {ECO:0000313|EMBL:CDD59461.1, ECO:0000313|Proteomes:UP000018284}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:43 {ECO:0000313|Proteomes:UP000018284};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000256|PROSITE-
CC       ProRule:PRU01240}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDD59461.1}.
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DR   EMBL; CBHG010000379; CDD59461.1; -; Genomic_DNA.
DR   AlphaFoldDB; R7B4K5; -.
DR   STRING; 1262805.BN653_00118; -.
DR   Proteomes; UP000018284; Unassembled WGS sequence.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07478; Peptidases_S8_CspA-like; 1.
DR   Gene3D; 2.60.120.1290; -; 1.
DR   Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR   InterPro; IPR034045; Pep_S8_CspA-like.
DR   InterPro; IPR017310; Pept_S8A_subtilisin_clostridia.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   PANTHER; PTHR43399; SUBTILISIN-RELATED; 1.
DR   PANTHER; PTHR43399:SF4; TK-SUBTILISIN; 1.
DR   Pfam; PF00082; Peptidase_S8; 2.
DR   PIRSF; PIRSF037894; Subtilisin_rel_CspABC; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF52743; Subtilisin-like; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00136; SUBTILASE_ASP; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU01240};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW   ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000018284};
KW   Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW   ProRule:PRU01240}.
FT   DOMAIN          94..293
FT                   /note="Peptidase S8/S53"
FT                   /evidence="ECO:0000259|Pfam:PF00082"
FT   DOMAIN          433..550
FT                   /note="Peptidase S8/S53"
FT                   /evidence="ECO:0000259|Pfam:PF00082"
FT   ACT_SITE        103
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        176
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        495
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
SQ   SEQUENCE   563 AA;  61825 MW;  801B1DD58D6E34EA CRC64;
     MNCSSLPDNQ TADFIERFFP FTYNQILDND SGCYDFVNHE YVIWHRSLAE ALPLSVQQYG
     YDSIPNLYTI LDTSSLDSAG ISRTAEQPFL GNKGRGALIG VVDTGIDYTN PLLRDPDGST
     RIAAIWDQTV PGAGLSTPDP IDALHYGSVY QRSHINEALR SEDPHSIVPT SDPLRHGTQM
     ALVAAGSEDA DAFFSGAAPE SELVVVRLKP ARTYLRDFFL IREDAIAFQE DDIMLGIKFL
     LYIARQEKKP LVILLGLGTN TGSHSGTSPL GLYLNSLSRA SGQILVAAGG NETGRGHHFR
     SVFPASTSYE DVELRVGPDE RGFTLELWAR ESELYSVGFL SPSGDETGRI RLSGNEERRI
     PFLLEGTTIH LNYTSSDLAT GSELILMRFD APAAGIWKIR VYHTLSIQGE YHMWLPVHGF
     ISDETFFLRP DPDTTITDPG NTAAIITTAA YSHQTGGIYL HSSRGFTRTR TIKPDLAAPG
     VRLPLPDYQE VTGTSFAAAM TAGAAAILLS WSAYTPSAPR INTSIAKAFL IRGADRRNSI
     EYPSREWGYG TLNLYNSFLS MRS
//