ID   R7ICL2_9FIRM            Unreviewed;       261 AA.
AC   R7ICL2;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 28.
DE   SubName: Full=Putative kanamycin kinase {ECO:0000313|EMBL:CDE49335.1};
GN   ORFNames=BN770_01982 {ECO:0000313|EMBL:CDE49335.1};
OS   Faecalibacterium sp. CAG:74.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Faecalibacterium.
OX   NCBI_TaxID=1262897 {ECO:0000313|EMBL:CDE49335.1, ECO:0000313|Proteomes:UP000018328};
RN   [1] {ECO:0000313|EMBL:CDE49335.1, ECO:0000313|Proteomes:UP000018328}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:74 {ECO:0000313|Proteomes:UP000018328};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000256|ARBA:ARBA00006219, ECO:0000256|PIRNR:PIRNR000706}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDE49335.1}.
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DR   EMBL; CBJB010000141; CDE49335.1; -; Genomic_DNA.
DR   AlphaFoldDB; R7ICL2; -.
DR   Proteomes; UP000018328; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.1200.10; -; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR024165; Kan/Strep_kinase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   PANTHER; PTHR21310; AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED; 1.
DR   PANTHER; PTHR21310:SF41; BLR5425 PROTEIN; 1.
DR   Pfam; PF01636; APH; 1.
DR   PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR   SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance {ECO:0000256|ARBA:ARBA00023251,
KW   ECO:0000256|PIRNR:PIRNR000706};
KW   ATP-binding {ECO:0000256|PIRNR:PIRNR000706};
KW   Kinase {ECO:0000256|PIRNR:PIRNR000706, ECO:0000313|EMBL:CDE49335.1};
KW   Magnesium {ECO:0000256|PIRSR:PIRSR000706-2};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR000706-2};
KW   Nucleotide-binding {ECO:0000256|PIRNR:PIRNR000706};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018328};
KW   Transferase {ECO:0000256|PIRNR:PIRNR000706}.
FT   DOMAIN          34..219
FT                   /note="Aminoglycoside phosphotransferase"
FT                   /evidence="ECO:0000259|Pfam:PF01636"
FT   ACT_SITE        179
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000706-1"
FT   BINDING         184
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000706-2"
FT   BINDING         197
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000706-2"
SQ   SEQUENCE   261 AA;  28745 MW;  329F7E6B8F27E430 CRC64;
     MIPAAIQALL PQYPGEENRI GHSGAQVVMY EDCVLKVQPD TGNAANEGIM LRFLKGRLPV
     PEVLAEAVQG GMRYLLMTRL RGRMLCDETF LDNQPLLARR MADAAEMLWR VDIDGCPCSY
     ALDDVLRKAE ADIAAGRVTI DTANQPETYG AGGFASPEAL LAWLKANRPP ETLVLSHGDF
     CLPNILADEK GIVGFLDLGQ AGCADRWRDL DQGLWSMWAN TTGLFGGRAR LFDRNALFSA
     LNLPLDEEKL RYYSLLSELC P
//