ID R7N2G3_9FIRM Unreviewed; 160 AA.
AC R7N2G3;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=Cyanate hydratase {ECO:0000256|HAMAP-Rule:MF_00535};
DE Short=Cyanase {ECO:0000256|HAMAP-Rule:MF_00535};
DE EC=4.2.1.104 {ECO:0000256|HAMAP-Rule:MF_00535};
DE AltName: Full=Cyanate hydrolase {ECO:0000256|HAMAP-Rule:MF_00535};
DE AltName: Full=Cyanate lyase {ECO:0000256|HAMAP-Rule:MF_00535};
GN Name=cynS {ECO:0000256|HAMAP-Rule:MF_00535};
GN ORFNames=BN816_01009 {ECO:0000313|EMBL:CDF06242.1};
OS Firmicutes bacterium CAG:95.
OC Bacteria; Bacillota.
OX NCBI_TaxID=1262988 {ECO:0000313|EMBL:CDF06242.1, ECO:0000313|Proteomes:UP000018182};
RN [1] {ECO:0000313|EMBL:CDF06242.1, ECO:0000313|Proteomes:UP000018182}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:95 {ECO:0000313|Proteomes:UP000018182};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the reaction of cyanate with bicarbonate to produce
CC ammonia and carbon dioxide. {ECO:0000256|ARBA:ARBA00003561,
CC ECO:0000256|HAMAP-Rule:MF_00535}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+);
CC Xref=Rhea:RHEA:11120, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:28938, ChEBI:CHEBI:29195;
CC EC=4.2.1.104; Evidence={ECO:0000256|HAMAP-Rule:MF_00535};
CC -!- SIMILARITY: Belongs to the cyanase family. {ECO:0000256|HAMAP-
CC Rule:MF_00535}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDF06242.1}.
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DR EMBL; CBKF010000027; CDF06242.1; -; Genomic_DNA.
DR AlphaFoldDB; R7N2G3; -.
DR STRING; 1262988.BN816_01009; -.
DR Proteomes; UP000018182; Unassembled WGS sequence.
DR GO; GO:0008824; F:cyanate hydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009439; P:cyanate metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd00093; HTH_XRE; 1.
DR Gene3D; 3.30.1160.10; Cyanate lyase, C-terminal domain; 1.
DR Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1.
DR HAMAP; MF_00535; Cyanate_hydrat; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR008076; Cyanase.
DR InterPro; IPR003712; Cyanate_lyase_C.
DR InterPro; IPR036581; Cyanate_lyase_C_sf.
DR InterPro; IPR048564; CYNS_N.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR NCBIfam; TIGR00673; cynS; 1.
DR PANTHER; PTHR34186; CYANATE HYDRATASE; 1.
DR PANTHER; PTHR34186:SF2; CYANATE HYDRATASE; 1.
DR Pfam; PF02560; Cyanate_lyase; 1.
DR Pfam; PF21291; CYNS_N; 1.
DR PRINTS; PR01693; CYANASE.
DR SMART; SM01116; Cyanate_lyase; 1.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF55234; Cyanase C-terminal domain; 1.
DR SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1.
DR PROSITE; PS50943; HTH_CROC1; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00535};
KW Reference proteome {ECO:0000313|Proteomes:UP000018182}.
FT DOMAIN 24..78
FT /note="HTH cro/C1-type"
FT /evidence="ECO:0000259|PROSITE:PS50943"
FT ACT_SITE 96
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00535"
FT ACT_SITE 99
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00535"
FT ACT_SITE 122
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00535"
SQ SEQUENCE 160 AA; 18110 MW; 254BE313A3A9355D CRC64;
MSMTPMNNFD YLERVKFSEE LAEIEAAKKA SGMTYEELAE KIGVNKVWLT SAIKGQQYVP
EEYCLKLAEV LGIDSKITMV LSEHPYKGNT DPVLYRLHEV LDTYGPAIKE IIHEKGGNAI
MSAIDFGIDV DIQKDPHGDR VIITWNGKLL PYSKQGQYPW
//
