ID R7XZL9_9ACTN Unreviewed; 641 AA.
AC R7XZL9;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=transketolase {ECO:0000256|ARBA:ARBA00013152};
DE EC=2.2.1.1 {ECO:0000256|ARBA:ARBA00013152};
GN ORFNames=CF8_1515 {ECO:0000313|EMBL:EON24475.1};
OS Nocardioides sp. CF8.
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Nocardioidaceae; Nocardioides.
OX NCBI_TaxID=110319 {ECO:0000313|EMBL:EON24475.1, ECO:0000313|Proteomes:UP000015971};
RN [1] {ECO:0000313|EMBL:EON24475.1, ECO:0000313|Proteomes:UP000015971}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CF8 {ECO:0000313|EMBL:EON24475.1,
RC ECO:0000313|Proteomes:UP000015971};
RX PubMed=23833136;
RA Kimbrel J.A., Chang J., Arp D.J., Sayavedra-Soto L.A.;
RT "The Draft Genome Sequence of Nocardioides sp. Strain CF8 Reveals the Scope
RT of Its Metabolic Capabilities.";
RL Genome Announc. 1:e00439-e00439(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate =
CC aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate;
CC Xref=Rhea:RHEA:10508, ChEBI:CHEBI:57483, ChEBI:CHEBI:57737,
CC ChEBI:CHEBI:58273, ChEBI:CHEBI:59776; EC=2.2.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001027};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
CC Evidence={ECO:0000256|ARBA:ARBA00001964};
CC -!- SIMILARITY: Belongs to the transketolase family.
CC {ECO:0000256|ARBA:ARBA00007131}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EON24475.1}.
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DR EMBL; ASEP01000048; EON24475.1; -; Genomic_DNA.
DR RefSeq; WP_010832477.1; NZ_CM001852.1.
DR AlphaFoldDB; R7XZL9; -.
DR STRING; 110319.CF8_1515; -.
DR PATRIC; fig|110319.3.peg.2103; -.
DR eggNOG; COG0021; Bacteria.
DR HOGENOM; CLU_009227_0_0_11; -.
DR OrthoDB; 8732661at2; -.
DR Proteomes; UP000015971; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProt.
DR GO; GO:0004802; F:transketolase activity; IEA:UniProtKB-EC.
DR CDD; cd07033; TPP_PYR_DXS_TK_like; 1.
DR CDD; cd02012; TPP_TK; 1.
DR Gene3D; 3.40.50.920; -; 1.
DR Gene3D; 3.40.50.970; -; 2.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR009014; Transketo_C/PFOR_II.
DR InterPro; IPR005475; Transketolase-like_Pyr-bd.
DR InterPro; IPR005478; Transketolase_bac-like.
DR InterPro; IPR049557; Transketolase_CS.
DR InterPro; IPR033247; Transketolase_fam.
DR InterPro; IPR005474; Transketolase_N.
DR NCBIfam; TIGR00232; tktlase_bact; 1.
DR PANTHER; PTHR43522; TRANSKETOLASE; 1.
DR PANTHER; PTHR43522:SF2; TRANSKETOLASE 1-RELATED; 1.
DR Pfam; PF02779; Transket_pyr; 1.
DR Pfam; PF00456; Transketolase_N; 1.
DR SMART; SM00861; Transket_pyr; 1.
DR SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
DR SUPFAM; SSF52922; TK C-terminal domain-like; 1.
DR PROSITE; PS00801; TRANSKETOLASE_1; 1.
PE 3: Inferred from homology;
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000015971};
KW Thiamine pyrophosphate {ECO:0000256|ARBA:ARBA00023052};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 357..526
FT /note="Transketolase-like pyrimidine-binding"
FT /evidence="ECO:0000259|SMART:SM00861"
SQ SEQUENCE 641 AA; 68065 MW; 20DF57AD4B823DCA CRC64;
MTDVLQTEHT TVETDRLAVD TLRFLAADMV QAANSGHPGM PMGAAPMAWA LWSRHLRHDP
ADPAWADRDR FVLSAGHGSA LLYGLLHIFG YDLPTEELRA FRQLGSRTPG HPEFGHTPGV
ECTTGPLGQG LAMAVGMALA ERMSAARFPR VTDHRTYAIV GDGCLMEGVS HEAGSFAGHL
GLGGLVVLWD DNSITIDGAV DRSCSDDQLA RFAAYGWHTE QVLDGTDVEA IHEAITRAEA
DPRPSFIAVR TVIGQGAPGV EGTSKAHGSP LGEELLAETK RLAGWDHEAF TVPEPVRAAC
AALAADGARA HAEWDKTFAA FADAEPEVAA QFTRALGRAL PADLEEVLGG AVGDAPRATR
QSSQACLNAL RDALPELVGG SADLAGSTGT SFGDVITRDD FGRRAIGFGI REFGMATFMN
GISLHGGFRV FGSTFLVFAD YLRPALRLSA LMKQPVVYVF THDSIAVGED GPTHQPITHI
ESLRIIPGLQ VLRPADDAET ARAWQLALER TDGPTALVLS RQSLPQLDRA TFESDSPVTL
EIVATGSEVA LAAAVAEVFR GEGYGVRVVS PFDRSRYTPD PSATRVSIEA GSTSAWSGIA
DVAIGIDDFG ASGPGEDVLA FHGFTVDNVV AKIREHLATR R
//
