ID R8ASB3_PLESH Unreviewed; 79 AA.
AC R8ASB3;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=Sulfur carrier protein TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur mediator TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur transfer protein TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000256|HAMAP-Rule:MF_00413};
GN Name=tusA {ECO:0000256|HAMAP-Rule:MF_00413};
GN ORFNames=PLESHI_07029 {ECO:0000313|EMBL:EON89215.1};
OS Plesiomonas shigelloides 302-73.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Plesiomonas.
OX NCBI_TaxID=1315976 {ECO:0000313|EMBL:EON89215.1, ECO:0000313|Proteomes:UP000014012};
RN [1] {ECO:0000313|EMBL:EON89215.1, ECO:0000313|Proteomes:UP000014012}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=302-73 {ECO:0000313|EMBL:EON89215.1,
RC ECO:0000313|Proteomes:UP000014012};
RX PubMed=23814109;
RA Pique N., Aquilini E., Alioto T., Minana-Galbis D., Tomas J.M.;
RT "Genome Sequence of Plesiomonas shigelloides Strain 302-73 (Serotype O1).";
RL Genome Announc. 1:e00404-13(2013).
CC -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in the
CC cell. Part of a sulfur-relay system required for 2-thiolation during
CC synthesis of 2-thiouridine of the modified wobble base 5-
CC methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts with
CC IscS and stimulates its cysteine desulfurase activity. Accepts an
CC activated sulfur from IscS, which is then transferred to TusD, and thus
CC determines the direction of sulfur flow from IscS to 2-thiouridine
CC formation. Also appears to be involved in sulfur transfer for the
CC biosynthesis of molybdopterin. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- PATHWAY: tRNA modification. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- SUBUNIT: Interacts with IscS. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC {ECO:0000256|ARBA:ARBA00008984, ECO:0000256|HAMAP-Rule:MF_00413}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EON89215.1}.
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DR EMBL; AQQO01000043; EON89215.1; -; Genomic_DNA.
DR RefSeq; WP_010863030.1; NZ_KB944507.1.
DR AlphaFoldDB; R8ASB3; -.
DR STRING; 703.SAMEA2665130_03052; -.
DR GeneID; 69704815; -.
DR PATRIC; fig|1315976.3.peg.1379; -.
DR HOGENOM; CLU_165255_5_1_6; -.
DR OrthoDB; 9797352at2; -.
DR Proteomes; UP000014012; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR CDD; cd03423; SirA; 1.
DR Gene3D; 3.30.110.40; TusA-like domain; 1.
DR HAMAP; MF_00413; Thiourid_synth_A; 1.
DR InterPro; IPR022931; Sulphur_carrier_TusA.
DR InterPro; IPR001455; TusA-like.
DR InterPro; IPR036868; TusA-like_sf.
DR PANTHER; PTHR33279:SF6; SULFUR CARRIER PROTEIN TUSA; 1.
DR PANTHER; PTHR33279; SULFUR CARRIER PROTEIN YEDF-RELATED; 1.
DR Pfam; PF01206; TusA; 1.
DR SUPFAM; SSF64307; SirA-like; 1.
DR PROSITE; PS01148; UPF0033; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413};
KW Reference proteome {ECO:0000313|Proteomes:UP000014012};
KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_00413}.
FT DOMAIN 10..34
FT /note="UPF0033"
FT /evidence="ECO:0000259|PROSITE:PS01148"
FT ACT_SITE 17
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00413"
SQ SEQUENCE 79 AA; 8974 MW; 58569C32B7F01B86 CRC64;
MNSVVPDHTL DAQGLRCPEP VMMVRKTIRN LTTGQTLLIV ADDPSTTRDI PSFCRFMDHQ
LLEAQTESLP YRYLIRKGA
//
