ID R8WUD7_9ENTR Unreviewed; 533 AA.
AC R8WUD7;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=cyclic-guanylate-specific phosphodiesterase {ECO:0000256|ARBA:ARBA00012282};
DE EC=3.1.4.52 {ECO:0000256|ARBA:ARBA00012282};
GN ORFNames=WC7_02475 {ECO:0000313|EMBL:EOQ48236.1};
OS Citrobacter sp. KTE151.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Citrobacter.
OX NCBI_TaxID=1169322 {ECO:0000313|EMBL:EOQ48236.1, ECO:0000313|Proteomes:UP000014006};
RN [1] {ECO:0000313|EMBL:EOQ48236.1, ECO:0000313|Proteomes:UP000014006}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KTE151 {ECO:0000313|EMBL:EOQ48236.1,
RC ECO:0000313|Proteomes:UP000014006};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Feldgarden M., Nielsen K.L., Frimodt-Moller N., Andersen P.S., Walker B.,
RA Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A.,
RA Alvarado L., Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J.,
RA Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A.,
RA Larimer J., McCowan C., Murphy C., Neiman D., Pearson M., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Escherichia coli KTE151.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3',3'-c-di-GMP + H2O = 5'-phosphoguanylyl(3'->5')guanosine +
CC H(+); Xref=Rhea:RHEA:24902, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58754, ChEBI:CHEBI:58805; EC=3.1.4.52;
CC Evidence={ECO:0000256|ARBA:ARBA00034290};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOQ48236.1}.
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DR EMBL; ASQK01000005; EOQ48236.1; -; Genomic_DNA.
DR RefSeq; WP_016153392.1; NZ_KB976064.1.
DR AlphaFoldDB; R8WUD7; -.
DR PATRIC; fig|1169322.3.peg.2471; -.
DR HOGENOM; CLU_000445_131_2_6; -.
DR Proteomes; UP000014006; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0071111; F:cyclic-guanylate-specific phosphodiesterase activity; IEA:UniProtKB-EC.
DR CDD; cd01948; EAL; 1.
DR Gene3D; 3.20.20.450; EAL domain; 1.
DR InterPro; IPR024744; CSS-motif_dom.
DR InterPro; IPR001633; EAL_dom.
DR InterPro; IPR035919; EAL_sf.
DR PANTHER; PTHR33121:SF9; CYCLIC DI-GMP PHOSPHODIESTERASE PDED-RELATED; 1.
DR PANTHER; PTHR33121; CYCLIC DI-GMP PHOSPHODIESTERASE PDEF; 1.
DR Pfam; PF12792; CSS-motif; 1.
DR Pfam; PF00563; EAL; 1.
DR SMART; SM00052; EAL; 1.
DR SUPFAM; SSF141868; EAL domain-like; 1.
DR PROSITE; PS50883; EAL; 1.
PE 4: Predicted;
KW c-di-GMP {ECO:0000256|ARBA:ARBA00022636};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT DOMAIN 266..515
FT /note="EAL"
FT /evidence="ECO:0000259|PROSITE:PS50883"
SQ SEQUENCE 533 AA; 59862 MW; 0D1DB87E619F356E CRC64;
MQTAQRIIKN YRRNRLIVCT LCALLTLILT LTVRFISERN LNHRNTVTFA NHAVEELDDV
LLPLQTGRDV LLPLIGLPCS VARLPLSKHA ARLQTVRSIS LIQSGILYCS SIFGYRNVPI
RQLEPELPSR EPQLLLSTDH SLIKGSPILT QWYPASADGS DGVLEIVNID LLSSMLLEPQ
PPQITHASLT VGNRHLLYGQ GVVDTLPELN NEKRYQLSSQ HFPFTISVTG PGASELAIKH
LPTQLPLAVI LSFLVGYLAW LATASRMSFS WEINLALAAR EFELFCQPLL NAQTQQCMGV
EILLRWNNPR QGWISPEVFI PIAEEHNLIV PLTRHVIAET IQQRHVFPMS SQFHIGINVA
ASHFRNGTLL KDLNQYWFSQ HPIQQLVIEL TERDALLDVD YRVVRELNRL GVKLAIDDFG
TGNSSLSWLE KLRPDVLKID KSFTSAIGTD AVNSTVTDII IALGKKLNIE LVAEGVETQA
QAQHLRRHGV HLLQGFLYAK PMPLRDFPKW LAGSTPPPAR HNGRHSMHVM PFR
//
