ID R9JLY2_9FIRM Unreviewed; 580 AA.
AC R9JLY2;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Rqc2 homolog RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
DE Short=RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN Name=rqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN ORFNames=C807_00880 {ECO:0000313|EMBL:EOS32157.1};
OS Lachnospiraceae bacterium 28-4.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=397287 {ECO:0000313|EMBL:EOS32157.1, ECO:0000313|Proteomes:UP000014112};
RN [1] {ECO:0000313|EMBL:EOS32157.1, ECO:0000313|Proteomes:UP000014112}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=28-4 {ECO:0000313|EMBL:EOS32157.1,
RC ECO:0000313|Proteomes:UP000014112};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Xavier R., Elson C., Duck W., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Lachnospiraceae bacterium 28-4.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Part of the ribosome quality control system (RQC). Recruits
CC Ala-charged tRNA and directs the elongation of stalled nascent chains
CC on 50S ribosomal subunits, leading to non-templated C-terminal Ala
CC extensions (Ala tail). The Ala tail promotes nascent chain degradation.
CC May add between 1 and at least 8 Ala residues. Binds to stalled 50S
CC ribosomal subunits. {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SUBUNIT: Associates with stalled 50S ribosomal subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SIMILARITY: Belongs to the NEMF family. {ECO:0000256|HAMAP-
CC Rule:MF_00844}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOS32157.1}.
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DR EMBL; ASSU01000011; EOS32157.1; -; Genomic_DNA.
DR AlphaFoldDB; R9JLY2; -.
DR STRING; 397287.C807_00880; -.
DR PATRIC; fig|397287.3.peg.939; -.
DR eggNOG; COG1293; Bacteria.
DR HOGENOM; CLU_022481_2_1_9; -.
DR OrthoDB; 9766163at2; -.
DR Proteomes; UP000014112; Unassembled WGS sequence.
DR GO; GO:0043023; F:ribosomal large subunit binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.8.50; -; 1.
DR Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR HAMAP; MF_00844_B; RqcH_B; 1.
DR InterPro; IPR008532; NFACT_RNA-bd.
DR InterPro; IPR010979; Ribosomal_uS13-like_H2TH.
DR InterPro; IPR043682; RqcH_bacterial.
DR PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR Pfam; PF05670; NFACT-R_1; 1.
DR Pfam; PF18297; NFACT-R_2; 1.
DR Pfam; PF05833; NFACT_N; 1.
DR SUPFAM; SSF46946; S13-like H2TH domain; 1.
PE 3: Inferred from homology;
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00844}; Reference proteome {ECO:0000313|Proteomes:UP000014112};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00844};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW Rule:MF_00844}.
FT DOMAIN 459..551
FT /note="NFACT RNA-binding"
FT /evidence="ECO:0000259|Pfam:PF05670"
SQ SEQUENCE 580 AA; 65782 MW; 4AB4A76848507A3D CRC64;
MAFDGVTIAG IVKELNDSLL GGRIYKIAQP ESDELMLTIK VNGGQKRLFI SASASLPLIY
LTENNKQSPM TAPGFCMLLR KHIQNGRIIG ISQPGLERIV QIQVEHLNEM GDLCRKNLVI
EIMGKHSNII FIDDKDMIID SIKHISGMVS SVREVLPGRP YFIPETQKKK NALDTNREEF
LQTILSGSQP VYKTLYASYT GFSPVFAQEL CFRAGLDGDA STASLSEDGM QKLYDVFQSA
VNDIQTGNFT PNIVYEFGSP VEFSVLPLTM YQAEERREEA SVSAMLERYY AQRNSISRIR
QKSADLRRIV QTALERNVKK YDLQLRQIKD TEKREKYRVY GELLNTYGYQ ASPGDPSITA
RNYYTDEDIT IPLDPTLAPL ENAKKYFEKY NKMKRTYEAL STLTLEVKEE IEHLESISAS
LDIAAAEEDL VQIREELVQN GYIRRKSGDK KVKITSRPFH YISSDGYHIY VGKNNLQNDE
LTFRFASGND WWFHAKGLPG SHVIVKTNGD TLPDRTFEEA AKLAAYYSRA RGQEKAEIDY
TEKKNVKKTN GGKPGFVIYH TNYSMVIDSD ISGLQQIRES
//
