ID   R9NX25_PSEHS            Unreviewed;       990 AA.
AC   R9NX25;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 40.
DE   RecName: Full=Calpain catalytic domain-containing protein {ECO:0000259|PROSITE:PS50203};
GN   ORFNames=PHSY_000686 {ECO:0000313|EMBL:GAC93124.1};
OS   Pseudozyma hubeiensis (strain SY62) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Pseudozyma.
OX   NCBI_TaxID=1305764 {ECO:0000313|EMBL:GAC93124.1, ECO:0000313|Proteomes:UP000014071};
RN   [1] {ECO:0000313|Proteomes:UP000014071}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SY62 {ECO:0000313|Proteomes:UP000014071};
RX   PubMed=23814110; DOI=10.1128/genomea.00409-13;
RA   Konishi M., Hatada Y., Horiuchi J.;
RT   "Draft genome sequence of the basidiomycetous yeast-like fungus Pseudozyma
RT   hubeiensis SY62, which produces an abundant amount of the biosurfactant
RT   mannosylerythritol lipids.";
RL   Genome Announc. 1:E0040913-E0040913(2013).
CC   -!- SIMILARITY: Belongs to the peptidase C2 family. PalB/RIM13 subfamily.
CC       {ECO:0000256|ARBA:ARBA00010193}.
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DR   EMBL; DF238772; GAC93124.1; -; Genomic_DNA.
DR   RefSeq; XP_012186711.1; XM_012331321.1.
DR   AlphaFoldDB; R9NX25; -.
DR   STRING; 1305764.R9NX25; -.
DR   GeneID; 24105990; -.
DR   eggNOG; KOG0045; Eukaryota.
DR   HOGENOM; CLU_006770_1_0_1; -.
DR   OrthoDB; 230045at2759; -.
DR   Proteomes; UP000014071; Unassembled WGS sequence.
DR   GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.380; -; 2.
DR   Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR   Gene3D; 1.20.58.80; Phosphotransferase system, lactose/cellobiose-type IIA subunit; 1.
DR   InterPro; IPR022683; Calpain_III.
DR   InterPro; IPR036213; Calpain_III_sf.
DR   InterPro; IPR036181; MIT_dom_sf.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR   PANTHER; PTHR46143; CALPAIN-7; 1.
DR   PANTHER; PTHR46143:SF1; CALPAIN-7; 1.
DR   Pfam; PF00648; Peptidase_C2; 1.
DR   SMART; SM00720; calpain_III; 2.
DR   SMART; SM00230; CysPc; 1.
DR   SUPFAM; SSF49758; Calpain large subunit, middle domain (domain III); 3.
DR   SUPFAM; SSF54001; Cysteine proteinases; 1.
DR   SUPFAM; SSF116846; MIT domain; 1.
DR   PROSITE; PS50203; CALPAIN_CAT; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU00239}; Protease {ECO:0000256|PROSITE-ProRule:PRU00239};
KW   Reference proteome {ECO:0000313|Proteomes:UP000014071};
KW   Thiol protease {ECO:0000256|PROSITE-ProRule:PRU00239}.
FT   DOMAIN          152..508
FT                   /note="Calpain catalytic"
FT                   /evidence="ECO:0000259|PROSITE:PS50203"
FT   REGION          275..296
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          602..624
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        209
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00239"
FT   ACT_SITE        413
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00239"
FT   ACT_SITE        433
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00239"
SQ   SEQUENCE   990 AA;  108429 MW;  F2685D9DF2B9BC07 CRC64;
     MSALITRAEW DSKLKTAQEQ AQRATKAELA RNYSEAFELY VRSGQLFVWL LGNLPNPNDQ
     HASENRASPF ASSSAAVDGC SEPAAAQTRE RLKQMATKVM ARAEKIKAIR KDLRPIDRDA
     LSQEEQSTIL VSSSLINGCR YPVWHASPSK APASKAATFT QEKQPPLSSI QLRKGAVYKR
     ASQLSSEEAQ GWSTKLAGSD IVQDIVSDCS FVAALEVAVQ HDRIFGGQLA TSALHPQDSS
     GRIQPSPDGQ YHVKLHINGA PRMVVIDDHL PCYPSTSTTS QASSSAPAPL PDQPNTRQHQ
     LMCVTSRDGQ LIWPALLEKA FLQVMGGYDF AGSNGSIDLY ALTGWLPEHI FLRHAGFQRE
     KTWIRFHDAW NAGRCMATAG TAKAPSRNQP VGSAQPGEAP ETIVLESGLV SSHNYAILDV
     RTFGSRRYVK LMNPWRTATG IRPAIAIPAE EERRVYAETA LNTISIEHKL SEMRLANEAE
     DDKATSTYTV SWDDFCSHFD SLFLNWDPEL FENSVTVHSS WRGTALLSTA HGSKQPDPHF
     KLSLTISPSM PRGSTDPTDE IWLLLTRHFT STHNAAEDAL PRSSEQGASD YIAIHAFEDD
     QRSAEGAQLA STPAPPSRTT KHKGAYVDGT HCLVRLKPSF LRKVDSNETS PRADSQQEPQ
     QCTFTVVVSR RSEGDALPSS AASPSEEAGK DVNYTLAVFS RYPMELSELR TRLPFCESVS
     GSWTARTAGG NATLASFMTN PQYTLVVPAG AGEVQLQVML ESSDRRIPVQ VLIAYPGAGT
     GRVTHLTEGD VVLSSGMYHH GLALCSTSRP GTSGTVVRPG SYTLIASTFA AGMEDEFHLR
     VESSRPVQVK PIPQEGAGMF HRRLTSSWRR GVTAHGSRAN QAYFDNPSWR FTIDRPGPTD
     FAARIRVEPM RGDDHVRPYV HLALFAEREG GWQQVANSGS YTDLVCGAAV EKVKLSPGTY
     RIVASTYKAE VEAQFGVDVY TERKVDLLQV
//