ID RECG_AQUAE Reviewed; 792 AA.
AC O67837;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 27-MAR-2024, entry version 129.
DE RecName: Full=ATP-dependent DNA helicase RecG;
DE EC=3.6.4.12;
GN Name=recG; OrderedLocusNames=aq_2053;
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificota; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
CC -!- FUNCTION: Critical role in recombination and DNA repair. Helps process
CC Holliday junction intermediates to mature products by catalyzing branch
CC migration. Has a DNA unwinding activity characteristic of a DNA
CC helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-
CC DNA) (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE000657; AAC07804.1; -; Genomic_DNA.
DR PIR; A70476; A70476.
DR RefSeq; NP_214406.1; NC_000918.1.
DR RefSeq; WP_010881342.1; NC_000918.1.
DR AlphaFoldDB; O67837; -.
DR SMR; O67837; -.
DR STRING; 224324.aq_2053; -.
DR EnsemblBacteria; AAC07804; AAC07804; aq_2053.
DR KEGG; aae:aq_2053; -.
DR PATRIC; fig|224324.8.peg.1583; -.
DR eggNOG; COG1200; Bacteria.
DR HOGENOM; CLU_005122_7_1_0; -.
DR InParanoid; O67837; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR CDD; cd17992; DEXHc_RecG; 1.
DR CDD; cd04488; RecG_wedge_OBF; 1.
DR CDD; cd18811; SF2_C_RecG; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR004609; ATP-dep_DNA_helicase_RecG.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR045562; RecG_dom3_C.
DR InterPro; IPR033454; RecG_wedge.
DR NCBIfam; TIGR00643; recG; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF19833; RecG_dom3_C; 1.
DR Pfam; PF17191; RecG_wedge; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT CHAIN 1..792
FT /note="ATP-dependent DNA helicase RecG"
FT /id="PRO_0000102137"
FT DOMAIN 375..538
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 557..719
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 489..492
FT /note="DEQH box"
FT BINDING 388..395
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 792 AA; 91180 MW; EFE1DC8BBAF38045 CRC64;
MDTAFVKDFI DNLLENDGLK LKRAIGVGIF LFNKLKEDAP DYILESLKEV DKLPFEKKKA
VLREVRKFLS EYEKRKKEGN FLEKTKRKPI DVFFNPIEKV KILTKTQIST LKALGIETVY
DALFYFPEKY EDKRLNTSIK TAKVGEKVAL KVKVKEVKIK ENERYTLEVV CTDGTGYITL
KYRYKNPHFA LKAFRKGMEI VVYGKLKSFK GEKYMVHPEV KSPSSEELGK IIPVYYVRKR
GELQEISSKT KQKRVRTALT ALSESLYRYF PEYMPDYLIE KYNFPDIALC IKELHNPKDI
SVNALNSFTD LYHKRVIYDE LFLFQLALLL KKQEIKKEKA PKVNVDEKFL RKAIEKLPFK
LTRAQERAIK EILEDLSRDV PMNRLLQGDV GSGKTIVAIL TSLAVVKSGY QVAVMVPTEI
LAHQHYKKFS EMLKDYGVNV ALLTGSLTPS QKKSVYKHVK EGNIHVLVGT HALIQDKVEF
KNLGYVIIDE QHRFGVMQRK LLLEKGKGLY PHCLVMSATP IPRTLALSIY GDLDISIIDE
LPPGRKEVIT KLYFESQKEE VYKKVEEELK KGNKVYVIYP LIEESEKLNL KAATEEYERW
KKLFPDRKVL LLHGKMPDKE KLAVMEEFKR EGDILVSTTV IEVGIDVPEA TVMVIEDAHR
FGLSQLHQLR GRVGRSDKEA YCLLVVPDEI KNEKNESLKR LRVFVKTTDG FKIAEEDLKL
RGPGEIIGVS QSGYFGFRVA NLARSQDRAL LGVARKDAEE LLKNNPNLEN LQDLKKLLIK
KYGDKMELSF VA
//
